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Literature for peptidase M50.004: RseP peptidase

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: K Knockout, P Specificity, I Inhibitor, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Asahi,K., Hirose,M., Aruga,R., Shimizu,Y., Tajiri,M., Tanaka,T., Adachi,Y., Tanaka,Y., Kaneko,M.K., Kato,Y., Akashi,S., Akiyama,Y., Hizukuri,Y., Kato,T. and Nogi,T.
    Cryo-EM structure of the bacterial intramembrane metalloprotease RseP in the substrate-bound state
    Sci Adv (2025) 11, eadu0925-eadu0925. PubMed  Europe PubMed DOI  PMC  EPMC
  2. Fremlen,H. and Burmann,B.M.
    Maintaining the Integral Membrane Proteome: Revisiting the Functional Repertoire of Integral Membrane Proteases
    Chembiochem (2025) , e202500048-e202500048. PubMed  Europe PubMed DOI  V
  3. Yokoyama,T., Miyazaki,R., Suzuki,T., Dohmae,N., Nagai,H., Tsukazaki,T., Kubori,T. and Akiyama,Y.
    Cleavage cascade of the sigma regulator FecR orchestrates TonB-dependent signal transduction
    Proc Natl Acad Sci U S A (2025) 122, e2500366122-e2500366122. PubMed  Europe PubMed DOI
    4. 2024
  4. Lou,X., Li,S., Wang,Y., Wang,R., Li,W., Yan,J., Zhang,Q., Liu,R. and Bartlam,M.
    Structural insights into regulated intramembrane proteolysis by the positive alginate regulator MucP from Pseudomonas aeruginosa
    Biochem Biophys Res Commun (2024) 740, 150999-150999. PubMed  Europe PubMed DOI
    5. 2023
  5. Yokoyama,T., Yamagata,Y., Honna,S., Mizuno,S., Katagiri,S., Oi,R., Nogi,T., Hizukuri,Y. and Akiyama,Y.
    S2P intramembrane protease RseP degrades small membrane proteins and suppresses the cytotoxicity of intrinsic toxin HokB
    MBio (2023) 14, e0108623-e0108623. PubMed  Europe PubMed DOI  PMC  EPMC
    6. 2022
  6. Kristensen,S.S., Oftedal,T.F., Rohr,A.K., Eijsink,V.G.H., Mathiesen,G. and Diep,D.B.
    The extracellular domain of site-2-metalloprotease RseP is important for sensitivity to bacteriocin EntK1
    J Biol Chem (2022) 298, 102593-102593. PubMed  Europe PubMed DOI  PMC  EPMC
    7. 2020
  7. Cheng,D., Lv,H., Yao,Y., Cheng,S., Huang,Q., Wang,H., Liu,X., Bae,T., Li,M. and Liu,Q.
    The roles of the site-2 protease Eep in Staphylococcus aureus
    J Bacteriol (2020) PubMed  Europe PubMed DOI
  8. Miyake,T., Hizukuri,Y. and Akiyama,Y.
    Involvement of a Membrane-Bound Amphiphilic Helix in Substrate Discrimination and Binding by an Escherichia coli S2P Peptidase RseP
    Front Microbiol (2020) 11, 607381-607381. PubMed  Europe PubMed DOI  PMC  EPMC
    9. 2018
  9. Delgado,C., Florez,L., Lollett,I., Lopez,C., Kangeyan,S., Kumari,H., Stylianou,M., Smiddy,R.J., Schneper,L., Sautter,R.T., Szatmari,G. and Mathee,K.
    Pseudomonas aeruginosa regulated intramembrane proteolysis (RIP): protease MucP can overcome mutations in the AlgO periplasmic protease to restore alginate production in nonmucoid revertants
    J Bacteriol (2018) 200, e00215-18-e00215-18. PubMed  Europe PubMed DOI
  10. Konovalova,A., Grabowicz,M., Balibar,C.J., Malinverni,J.C., Painter,R.E., Riley,D., Mann,P.A., Wang,H., Garlisi,C.G., Sherborne,B., Rigel,N.W., Ricci,D.P., Black,T.A., Roemer,T., Silhavy,T.J. and Walker,S.S.
    Inhibitor of intramembrane protease RseP blocks the sigma(E) response causing lethal accumulation of unfolded outer membrane proteins
    Proc Natl Acad Sci U S A (2018) 115, E6614-E6614. PubMed  Europe PubMed DOI  I
    11. 2017
  11. Akiyama,K., Hizukuri,Y. and Akiyama,Y.
    Involvement of a conserved GFG motif region in substrate binding by RseP, an Escherichia coli S2P protease
    Mol Microbiol (2017) 104, 737-751. PubMed  Europe PubMed DOI  P
  12. Bastiaansen,K.C., Civantos,C., Bitter,W. and Llamas,M.A.
    New insights into the regulation of cell-surface signaling activity acquired from a mutagenesis screen of the Pseudomonas putida IutY sigma/anti-sigma factor
    Front Microbiol (2017) 8, 747-747. PubMed  Europe PubMed DOI  PMC  EPMC
  13. Hizukuri,Y., Akiyama,K. and Akiyama,Y.
    Biochemical characterization of function and structure of RseP, an Escherichia coli S2P protease
    Methods Enzymol (2017) 584, 1-33. PubMed  Europe PubMed DOI
  14. Ovchinnikov,K.V., Kristiansen,P.E., Straume,D., Jensen,M.S., Aleksandrzak-Piekarczyk,T., Nes,I.F. and Diep,D.B.
    The leaderless bacteriocin enterocin K1 is highly potent against Enterococcus faecium: a study on structure, target spectrum and receptor
    Front Microbiol (2017) 8, 774-774. PubMed  Europe PubMed DOI  PMC  EPMC
    15. 2015
  15. Akiyama,K., Mizuno,S., Hizukuri,Y., Mori,H., Nogi,T. and Akiyama,Y.
    Roles of the membrane-reentrant beta-hairpin-like loop of RseP protease in selective substrate cleavage
    elife (2015) 4, e08928- PubMed  Europe PubMed DOI  PMC  EPMC
  16. Bastiaansen,K.C., Otero-Asman,J.R., Luirink,J., Bitter,W. and Llamas,M.A.
    Processing of cell-surface signalling anti-sigma factors prior to signal recognition is a conserved autoproteolytic mechanism that produces two functional domains
    Environ Microbiol (2015) 17, 3263-3277. PubMed  Europe PubMed DOI
  17. Teoh,W.P., Matson,J.S. and DiRita,V.J.
    Regulated intramembrane proteolysis of the virulence activator TcpP in Vibrio cholerae is initiated by the tail-specific protease (Tsp)
    Mol Microbiol (2015) 97, 822-831. PubMed  Europe PubMed DOI
    18. 2014
  18. Bastiaansen,K.C., Ibanez,A., Ramos,J.L., Bitter,W. and Llamas,M.A.
    The Prc and RseP proteases control bacterial cell-surface signalling activity
    Environ Microbiol (2014) 16, 2433-2443. PubMed  Europe PubMed DOI
  19. Hizukuri,Y., Oda,T., Tabata,S., Tamura-Kawakami,K., Oi,R., Sato,M., Takagi,J., Akiyama,Y. and Nogi,T.
    A structure-based model of substrate discrimination by a noncanonical PDZ tandem in the intramembrane-cleaving protease RseP
    Structure (2014) 22, 326-336. PubMed  Europe PubMed DOI
    20. 2013
  20. Barchinger,S.E. and Ades,S.E.
    Regulated proteolysis: control of the Escherichia coli sigma(E)-dependent cell envelope stress response
    Subcell Biochem (2013) 66, 129-160. PubMed  Europe PubMed DOI  V
  21. Frank,K.L., Guiton,P.S., Barnes,A.M., Manias,D.A., Chuang-Smith,O.N., Kohler,P.L., Spaulding,A.R., Hultgren,S.J., Schlievert,P.M. and Dunny,G.M.
    AhrC and Eep are biofilm infection-associated virulence factors in Enterococcus faecalis
    Infect Immun (2013) 81, 1696-1708. PubMed  Europe PubMed DOI
  22. Kroos,L. and Akiyama,Y.
    Biochemical and structural insights into intramembrane metalloprotease mechanisms
    Biochim Biophys Acta (2013) 1828, 2873-2885. PubMed  Europe PubMed DOI  PMC  EPMC  V
  23. Nuss,A.M., Adnan,F., Weber,L., Berghoff,B.A., Glaeser,J. and Klug,G.
    DegS and RseP homologous proteases are involved in singlet oxygen dependent activation of RpoE in Rhodobacter sphaeroides
    PLoS ONE (2013) 8, e79520-e79520. PubMed  Europe PubMed DOI
  24. Uzelac,G., Kojic,M., Lozo,J., Aleksandrzak-Piekarczyk,T., Gabrielsen,C., Kristensen,T., Nes,I.F., Diep,D.B. and Topisirovic,L.
    A Zn-dependent metallopeptidase is responsible for sensitivity to LsbB, a class II leaderless bacteriocin of Lactococcus lactis subsp. lactis BGMN1-5
    J Bacteriol (2013) 195, 5614-5621. PubMed  Europe PubMed DOI
  25. Varahan,S., Iyer,V.S., Moore,W.T. and Hancock,L.E.
    Eep confers lysozyme resistance to Enterococcus faecalis via the activation of the extracytoplasmic function sigma factor SigV
    J Bacteriol (2013) 195, 3125-3134. PubMed  Europe PubMed DOI
    26. 2012
  26. Frank,K.L., Barnes,A.M., Grindle,S.M., Manias,D.A., Schlievert,P.M. and Dunny,G.M.
    Use of recombinase-based in vivo expression technology to characterize Enterococcus faecalis gene expression during infection identifies in vivo-expressed antisense RNAs and implicates the protease Eep in pathogenesis
    Infect Immun (2012) 80, 539-549. PubMed  Europe PubMed DOI  PMC  EPMC  K
  27. Hizukuri,Y., Ito,K. and Akiyama,Y.
    RseP peptidase
    [ISSN:978-0-12-407743-0] (2012) 3, 1545-1550. DOI
  28. Hizukuri,Y. and Akiyama,Y.
    PDZ domains of RseP are not essential for sequential cleavage of RseA or stress-induced sigma(E) activation in vivo
    Mol Microbiol (2012) 86, 1232-1245. PubMed  Europe PubMed DOI
  29. Khan,R., Rukke,H.V., Ricomini Filho,A.P., Fimland,G., Arntzen,M.O., Thiede,B. and Petersen,F.C.
    Extracellular identification of a processed type II ComR/ComS pheromone of Streptococcus mutans
    J Bacteriol (2012) 194, 3781-3788. PubMed  Europe PubMed DOI
    30. 2011
  30. Damron,F.H. and Yu,H.D.
    Pseudomonas aeruginosa MucD regulates the alginate pathway through activation of MucA degradation via MucP proteolytic activity
    J Bacteriol (2011) 193, 286-291. PubMed  Europe PubMed DOI
  31. Saito,A., Hizukuri,Y., Matsuo,E., Chiba,S., Mori,H., Nishimura,O., Ito,K. and Akiyama,Y.
    Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria
    Proc Natl Acad Sci U S A (2011) 108, 13740-13745. PubMed  Europe PubMed DOI
    32. 2010
  32. Vickerman,M.M., Flannagan,S.E., Jesionowski,A.M., Brossard,K.A., Clewell,D.B. and Sedgley,C.M.
    A genetic determinant in Streptococcus gordonii Challis encodes a peptide with activity similar to that of enterococcal sex pheromone cAM373, which facilitates intergeneric DNA transfer
    J Bacteriol (2010) 192, 2535-2545. PubMed  Europe PubMed DOI
    33. 2009
  33. Heinrich,J. and Wiegert,T.
    Regulated intramembrane proteolysis in the control of extracytoplasmic function sigma factors
    Res Microbiol (2009) 160, 696-703. PubMed  Europe PubMed DOI
  34. Li,X., Wang,B., Feng,L., Kang,H., Qi,Y., Wang,J. and Shi,Y.
    Cleavage of RseA by RseP requires a carboxyl-terminal hydrophobic amino acid following DegS cleavage
    Proc Natl Acad Sci U S A (2009) 106, 14837-14842. PubMed  Europe PubMed DOI  PMC  EPMC
  35. Muller,C., Bang,I.S., Velayudhan,J., Karlinsey,J., Papenfort,K., Vogel,J. and Fang,F.C.
    Acid stress activation of the sigma(E) stress response in Salmonella enterica serovar Typhimurium
    Mol Microbiol (2009) 71, 1228-1238. PubMed  Europe PubMed DOI
  36. Wood,L.F. and Ohman,D.E.
    Use of cell wall stress to characterize sigma22 (AlgT/U) activation by regulated proteolysis and its regulon in Pseudomonas aeruginosa
    Mol Microbiol (2009) 72, 183-201. PubMed  Europe PubMed DOI
    37. 2008
  37. Ades,S.E.
    Regulation by destruction: design of the sigmaE envelope stress response
    Curr Opin Microbiol (2008) 11, 535-540. PubMed  Europe PubMed DOI
  38. Chandler,J.R. and Dunny,G.M.
    Characterization of the sequence specificity determinants required for processing and control of sex pheromone by the intramembrane protease Eep and the plasmid-encoded protein PrgY
    J Bacteriol (2008) 190, 1172-1183. PubMed  Europe PubMed DOI  PMC  EPMC
  39. Denham,E.L., Ward,P.N. and Leigh,J.A.
    Lipoprotein signal peptides are processed by Lsp and Eep of Streptococcus uberis
    J Bacteriol (2008) 190, 4641-4647. PubMed  Europe PubMed DOI  PMC  EPMC
  40. Inaba,K., Suzuki,M., Maegawa,K., Akiyama,S., Ito,K. and Akiyama,Y.
    A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of Escherichia coli
    J Biol Chem (2008) 283, 35042-35052. PubMed  Europe PubMed DOI
  41. Koide,K., Ito,K. and Akiyama,Y.
    Substrate recognition and binding by RseP, an Escherichia coli intramembrane protease
    J Biol Chem (2008) 283, 9562-9570. PubMed  Europe PubMed DOI
    42. 2007
  42. Cezairliyan,B.O. and Sauer,R.T.
    Inhibition of regulated proteolysis by RseB
    Proc Natl Acad Sci U S A (2007) 104, 3771-3776. PubMed  Europe PubMed DOI  PMC  EPMC
  43. Chaba,R., Grigorova,I.L., Flynn,J.M., Baker,T.A. and Gross,C.A.
    Design principles of the proteolytic cascade governing the sigmaE-mediated envelope stress response in Escherichia coli: keys to graded, buffered, and rapid signal transduction
    Genes Dev (2007) 21, 124-136. PubMed  Europe PubMed DOI  PMC  EPMC
  44. [YEAR:1-1-2007]Hasenbein,S., Merdanovic,M. and Ehrmann,M.
    Determinants of regulated proteolysis in signal transduction
    Genes Dev (1-1-2007) 21, 6-10. PubMed  Europe PubMed DOI
  45. King-Lyons,N.D., Smith,K.F. and Connell,T.D.
    Expression of hurP, a gene encoding a prospective site 2 protease, is essential for heme-dependent induction of bhuR in Bordetella bronchiseptica
    J Bacteriol (2007) 189, 6266-6275. PubMed  Europe PubMed DOI  PMC  EPMC
  46. Koide,K., Maegawa,S., Ito,K. and Akiyama,Y.
    Environment of the active site region of RseP, an Escherichia coli regulated intramembrane proteolysis protease, assessed by site-directed cysteine alkylation
    J Biol Chem (2007) 282, 4553-4560. PubMed  Europe PubMed DOI
  47. Qiu,D., Eisinger,V.M., Rowen,D.W. and Yu,H.D.
    Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa
    Proc Natl Acad Sci U S A (2007) 104, 8107-8112. PubMed  Europe PubMed DOI  PMC  EPMC
    48. 2006
  48. [YEAR:2-3-2006]Douchin,V., Bohn,C. and Bouloc,P.
    Down-regulation of porins by a small RNA bypasses the essentiality of the regulated intramembrane proteolysis protease RseP in Escherichia coli
    J Biol Chem (2-3-2006) 281, 12253-12259. PubMed  Europe PubMed DOI
    49. 2005
  49. Chen,J.C., Viollier,P.H. and Shapiro,L.
    A membrane metalloprotease participates in the sequential degradation of a Caulobacter polarity determinant
    Mol Microbiol (2005) 55, 1085-1103. PubMed  Europe PubMed DOI
  50. [YEAR:27-10-2005]Matson,J.S. and DiRita,V.J.
    Degradation of the membrane-localized virulence activator TcpP by the YaeL protease in Vibrio cholerae
    Proc Natl Acad Sci U S A (27-10-2005) 102, 16403-16408. PubMed  Europe PubMed DOI  PMC  EPMC
    51. 2004
  51. [YEAR:10-12-2004]Akiyama,Y., Kanehara,K. and Ito,K.
    RseP (YaeL), an Escherichia coli RIP protease, cleaves transmembrane sequences
    EMBO J (10-12-2004) 23, 4434-4442. PubMed  Europe PubMed DOI  PMC  EPMC
  52. Bohn,C., Collier,J. and Bouloc,P.
    Dispensable PDZ domain of Escherichia coli YaeL essential protease
    Mol Microbiol (2004) 52, 427-435. PubMed  Europe PubMed DOI
  53. Flynn,J.M., Levchenko,I., Sauer,R.T. and Baker,T.A.
    Modulating substrate choice: the SspB adaptor delivers a regulator of the extracytoplasmic-stress response to the AAA+ protease ClpXP for degradation
    Genes Dev (2004) 18, 2292-2301. PubMed  Europe PubMed DOI  PMC  EPMC
  54. [YEAR:1-11-2004]Grigorova,I.L., Chaba,R., Zhong,H.J., Alba,B.M., Rhodius,V., Herman,C. and Gross,C.A.
    Fine-tuning of the Escherichia coli sigmaE envelope stress response relies on multiple mechanisms to inhibit signal-independent proteolysis of the transmembrane anti-sigma factor, RseA
    Genes Dev (1-11-2004) 18, 2686-2697. PubMed  Europe PubMed DOI  PMC  EPMC
  55. Rawson,R.B.
    S2P protease
    [ISSN:0-12-079610-4] (2004) 2, 985-988.  V
    56. 2003
  56. Kanehara,K., Ito,K. and Akiyama,Y.
    YaeL proteolysis of RseA is controlled by the PDZ domain of YaeL and a Gln-rich region of RseA
    EMBO J (2003) 22, 6389-6398. PubMed  Europe PubMed DOI  PMC  EPMC
    57. 2002
  57. [YEAR:15-8-2002]Alba,B.M., Leeds,J.A., Onufryk,C., Lu,C.Z. and Gross,C.A.
    DegS and YaeL participate sequentially in the cleavage of RseA to activate the sigma(E)-dependent extracytoplasmic stress response
    Genes Dev (15-8-2002) 16, 2156-2168. PubMed  Europe PubMed DOI  PMC  EPMC
  58. Drew,D., Sjostrand,D., Nilsson,J., Urbig,T., Chin,C.N., de Gier,J.W. and von Heijne,G.
    Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis
    Proc Natl Acad Sci U S A (2002) 99, 2690-2695. PubMed  Europe PubMed DOI  PMC  EPMC
  59. [YEAR:15-8-2002]Kanehara,K., Ito,K. and Akiyama,Y.
    YaeL (EcfE) activates the sigma(E) pathway of stress response through a site-2 cleavage of anti-sigma(E), RseA
    Genes Dev (15-8-2002) 16, 2147-2155. PubMed  Europe PubMed DOI  PMC  EPMC
    60. 2001
  60. [YEAR:1-11-2001]Dartigalongue,C., Loferer,H. and Raina,S.
    EcfE, a new essential inner membrane protease: its role in the regulation of heat shock response in Escherichia coli
    EMBO J (1-11-2001) 20, 5908-5918. PubMed  Europe PubMed DOI  PMC  EPMC
  61. [YEAR:27-12-2001]Kanehara,K., Akiyama,Y. and Ito,K.
    Characterization of the yaeL gene product and its S2P-protease motifs in Escherichia coli
    Gene (27-12-2001) 281, 71-79. PubMed  Europe PubMed DOI
    62. 1999
  62. An,F.Y., Sulavik,M.C. and Clewell,D.B.
    Identification and characterization of a determinant (eep) on the Enterococcus faecalis chromosome that is involved in production of the peptide sex pheromone cAD1
    J Bacteriol (1999) 181, 5915-5921. PubMed  Europe PubMed  PMC  EPMC

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