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Literature for peptidase M50.004: RseP peptidase

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: K Knockout, P Specificity, I Inhibitor, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Asahi,K., Hirose,M., Aruga,R., Shimizu,Y., Tajiri,M., Tanaka,T., Adachi,Y., Tanaka,Y., Kaneko,M.K., Kato,Y., Akashi,S., Akiyama,Y., Hizukuri,Y., Kato,T. and Nogi,T.<br>Cryo-EM structure of the bacterial intramembrane metalloprotease RseP in the substrate-bound state<br>Sci Adv (2025) 11, eadu0925-eadu0925. PubMed  Europe PubMed DOI  PMC  EPMC
  2. Fremlen,H. and Burmann,B.M.<br>Maintaining the Integral Membrane Proteome: Revisiting the Functional Repertoire of Integral Membrane Proteases<br>Chembiochem (2025) , e202500048-e202500048. PubMed  Europe PubMed DOI  V
  3. Yokoyama,T., Miyazaki,R., Suzuki,T., Dohmae,N., Nagai,H., Tsukazaki,T., Kubori,T. and Akiyama,Y.<br>Cleavage cascade of the sigma regulator FecR orchestrates TonB-dependent signal transduction<br>Proc Natl Acad Sci U S A (2025) 122, e2500366122-e2500366122. PubMed  Europe PubMed DOI
    4. 2024
  4. Lou,X., Li,S., Wang,Y., Wang,R., Li,W., Yan,J., Zhang,Q., Liu,R. and Bartlam,M.<br>Structural insights into regulated intramembrane proteolysis by the positive alginate regulator MucP from Pseudomonas aeruginosa<br>Biochem Biophys Res Commun (2024) 740, 150999-150999. PubMed  Europe PubMed DOI
    5. 2023
  5. Yokoyama,T., Yamagata,Y., Honna,S., Mizuno,S., Katagiri,S., Oi,R., Nogi,T., Hizukuri,Y. and Akiyama,Y.<br>S2P intramembrane protease RseP degrades small membrane proteins and suppresses the cytotoxicity of intrinsic toxin HokB<br>MBio (2023) 14, e0108623-e0108623. PubMed  Europe PubMed DOI  PMC  EPMC
    6. 2022
  6. Kristensen,S.S., Oftedal,T.F., Rohr,A.K., Eijsink,V.G.H., Mathiesen,G. and Diep,D.B.<br>The extracellular domain of site-2-metalloprotease RseP is important for sensitivity to bacteriocin EntK1<br>J Biol Chem (2022) 298, 102593-102593. PubMed  Europe PubMed DOI  PMC  EPMC
    7. 2020
  7. Cheng,D., Lv,H., Yao,Y., Cheng,S., Huang,Q., Wang,H., Liu,X., Bae,T., Li,M. and Liu,Q.<br>The roles of the site-2 protease Eep in Staphylococcus aureus<br>J Bacteriol (2020) PubMed  Europe PubMed DOI
  8. Miyake,T., Hizukuri,Y. and Akiyama,Y.<br>Involvement of a Membrane-Bound Amphiphilic Helix in Substrate Discrimination and Binding by an Escherichia coli S2P Peptidase RseP<br>Front Microbiol (2020) 11, 607381-607381. PubMed  Europe PubMed DOI  PMC  EPMC
    9. 2018
  9. Delgado,C., Florez,L., Lollett,I., Lopez,C., Kangeyan,S., Kumari,H., Stylianou,M., Smiddy,R.J., Schneper,L., Sautter,R.T., Szatmari,G. and Mathee,K.<br>Pseudomonas aeruginosa regulated intramembrane proteolysis (RIP): protease MucP can overcome mutations in the AlgO periplasmic protease to restore alginate production in nonmucoid revertants<br>J Bacteriol (2018) 200, e00215-18-e00215-18. PubMed  Europe PubMed DOI
  10. Konovalova,A., Grabowicz,M., Balibar,C.J., Malinverni,J.C., Painter,R.E., Riley,D., Mann,P.A., Wang,H., Garlisi,C.G., Sherborne,B., Rigel,N.W., Ricci,D.P., Black,T.A., Roemer,T., Silhavy,T.J. and Walker,S.S.<br>Inhibitor of intramembrane protease RseP blocks the sigma(E) response causing lethal accumulation of unfolded outer membrane proteins<br>Proc Natl Acad Sci U S A (2018) 115, E6614-E6614. PubMed  Europe PubMed DOI  I
    11. 2017
  11. Akiyama,K., Hizukuri,Y. and Akiyama,Y.<br>Involvement of a conserved GFG motif region in substrate binding by RseP, an Escherichia coli S2P protease<br>Mol Microbiol (2017) 104, 737-751. PubMed  Europe PubMed DOI  P
  12. Bastiaansen,K.C., Civantos,C., Bitter,W. and Llamas,M.A.<br>New insights into the regulation of cell-surface signaling activity acquired from a mutagenesis screen of the Pseudomonas putida IutY sigma/anti-sigma factor<br>Front Microbiol (2017) 8, 747-747. PubMed  Europe PubMed DOI  PMC  EPMC
  13. Hizukuri,Y., Akiyama,K. and Akiyama,Y.<br>Biochemical characterization of function and structure of RseP, an Escherichia coli S2P protease<br>Methods Enzymol (2017) 584, 1-33. PubMed  Europe PubMed DOI
  14. Ovchinnikov,K.V., Kristiansen,P.E., Straume,D., Jensen,M.S., Aleksandrzak-Piekarczyk,T., Nes,I.F. and Diep,D.B.<br>The leaderless bacteriocin enterocin K1 is highly potent against Enterococcus faecium: a study on structure, target spectrum and receptor<br>Front Microbiol (2017) 8, 774-774. PubMed  Europe PubMed DOI  PMC  EPMC
    15. 2015
  15. Akiyama,K., Mizuno,S., Hizukuri,Y., Mori,H., Nogi,T. and Akiyama,Y.<br>Roles of the membrane-reentrant beta-hairpin-like loop of RseP protease in selective substrate cleavage<br>elife (2015) 4, e08928- PubMed  Europe PubMed DOI  PMC  EPMC
  16. Bastiaansen,K.C., Otero-Asman,J.R., Luirink,J., Bitter,W. and Llamas,M.A.<br>Processing of cell-surface signalling anti-sigma factors prior to signal recognition is a conserved autoproteolytic mechanism that produces two functional domains<br>Environ Microbiol (2015) 17, 3263-3277. PubMed  Europe PubMed DOI
  17. Teoh,W.P., Matson,J.S. and DiRita,V.J.<br>Regulated intramembrane proteolysis of the virulence activator TcpP in Vibrio cholerae is initiated by the tail-specific protease (Tsp)<br>Mol Microbiol (2015) 97, 822-831. PubMed  Europe PubMed DOI
    18. 2014
  18. Bastiaansen,K.C., Ibanez,A., Ramos,J.L., Bitter,W. and Llamas,M.A.<br>The Prc and RseP proteases control bacterial cell-surface signalling activity<br>Environ Microbiol (2014) 16, 2433-2443. PubMed  Europe PubMed DOI
  19. Hizukuri,Y., Oda,T., Tabata,S., Tamura-Kawakami,K., Oi,R., Sato,M., Takagi,J., Akiyama,Y. and Nogi,T.<br>A structure-based model of substrate discrimination by a noncanonical PDZ tandem in the intramembrane-cleaving protease RseP<br>Structure (2014) 22, 326-336. PubMed  Europe PubMed DOI
    20. 2013
  20. Barchinger,S.E. and Ades,S.E.<br>Regulated proteolysis: control of the Escherichia coli sigma(E)-dependent cell envelope stress response<br>Subcell Biochem (2013) 66, 129-160. PubMed  Europe PubMed DOI  V
  21. Frank,K.L., Guiton,P.S., Barnes,A.M., Manias,D.A., Chuang-Smith,O.N., Kohler,P.L., Spaulding,A.R., Hultgren,S.J., Schlievert,P.M. and Dunny,G.M.<br>AhrC and Eep are biofilm infection-associated virulence factors in Enterococcus faecalis<br>Infect Immun (2013) 81, 1696-1708. PubMed  Europe PubMed DOI
  22. Kroos,L. and Akiyama,Y.<br>Biochemical and structural insights into intramembrane metalloprotease mechanisms<br>Biochim Biophys Acta (2013) 1828, 2873-2885. PubMed  Europe PubMed DOI  PMC  EPMC  V
  23. Nuss,A.M., Adnan,F., Weber,L., Berghoff,B.A., Glaeser,J. and Klug,G.<br>DegS and RseP homologous proteases are involved in singlet oxygen dependent activation of RpoE in Rhodobacter sphaeroides<br>PLoS ONE (2013) 8, e79520-e79520. PubMed  Europe PubMed DOI
  24. Uzelac,G., Kojic,M., Lozo,J., Aleksandrzak-Piekarczyk,T., Gabrielsen,C., Kristensen,T., Nes,I.F., Diep,D.B. and Topisirovic,L.<br>A Zn-dependent metallopeptidase is responsible for sensitivity to LsbB, a class II leaderless bacteriocin of Lactococcus lactis subsp. lactis BGMN1-5<br>J Bacteriol (2013) 195, 5614-5621. PubMed  Europe PubMed DOI
  25. Varahan,S., Iyer,V.S., Moore,W.T. and Hancock,L.E.<br>Eep confers lysozyme resistance to Enterococcus faecalis via the activation of the extracytoplasmic function sigma factor SigV<br>J Bacteriol (2013) 195, 3125-3134. PubMed  Europe PubMed DOI
    26. 2012
  26. Frank,K.L., Barnes,A.M., Grindle,S.M., Manias,D.A., Schlievert,P.M. and Dunny,G.M.<br>Use of recombinase-based in vivo expression technology to characterize Enterococcus faecalis gene expression during infection identifies in vivo-expressed antisense RNAs and implicates the protease Eep in pathogenesis<br>Infect Immun (2012) 80, 539-549. PubMed  Europe PubMed DOI  PMC  EPMC  K
  27. Hizukuri,Y., Ito,K. and Akiyama,Y.<br>RseP peptidase<br>[ISSN:978-0-12-407743-0] (2012) 3, 1545-1550. DOI
  28. Hizukuri,Y. and Akiyama,Y.<br>PDZ domains of RseP are not essential for sequential cleavage of RseA or stress-induced sigma(E) activation in vivo<br>Mol Microbiol (2012) 86, 1232-1245. PubMed  Europe PubMed DOI
  29. Khan,R., Rukke,H.V., Ricomini Filho,A.P., Fimland,G., Arntzen,M.O., Thiede,B. and Petersen,F.C.<br>Extracellular identification of a processed type II ComR/ComS pheromone of Streptococcus mutans<br>J Bacteriol (2012) 194, 3781-3788. PubMed  Europe PubMed DOI
    30. 2011
  30. Damron,F.H. and Yu,H.D.<br>Pseudomonas aeruginosa MucD regulates the alginate pathway through activation of MucA degradation via MucP proteolytic activity<br>J Bacteriol (2011) 193, 286-291. PubMed  Europe PubMed DOI
  31. Saito,A., Hizukuri,Y., Matsuo,E., Chiba,S., Mori,H., Nishimura,O., Ito,K. and Akiyama,Y.<br>Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria<br>Proc Natl Acad Sci U S A (2011) 108, 13740-13745. PubMed  Europe PubMed DOI
    32. 2010
  32. Vickerman,M.M., Flannagan,S.E., Jesionowski,A.M., Brossard,K.A., Clewell,D.B. and Sedgley,C.M.<br>A genetic determinant in Streptococcus gordonii Challis encodes a peptide with activity similar to that of enterococcal sex pheromone cAM373, which facilitates intergeneric DNA transfer<br>J Bacteriol (2010) 192, 2535-2545. PubMed  Europe PubMed DOI
    33. 2009
  33. Heinrich,J. and Wiegert,T.<br>Regulated intramembrane proteolysis in the control of extracytoplasmic function sigma factors<br>Res Microbiol (2009) 160, 696-703. PubMed  Europe PubMed DOI
  34. Li,X., Wang,B., Feng,L., Kang,H., Qi,Y., Wang,J. and Shi,Y.<br>Cleavage of RseA by RseP requires a carboxyl-terminal hydrophobic amino acid following DegS cleavage<br>Proc Natl Acad Sci U S A (2009) 106, 14837-14842. PubMed  Europe PubMed DOI  PMC  EPMC
  35. Muller,C., Bang,I.S., Velayudhan,J., Karlinsey,J., Papenfort,K., Vogel,J. and Fang,F.C.<br>Acid stress activation of the sigma(E) stress response in Salmonella enterica serovar Typhimurium<br>Mol Microbiol (2009) 71, 1228-1238. PubMed  Europe PubMed DOI
  36. Wood,L.F. and Ohman,D.E.<br>Use of cell wall stress to characterize sigma22 (AlgT/U) activation by regulated proteolysis and its regulon in Pseudomonas aeruginosa<br>Mol Microbiol (2009) 72, 183-201. PubMed  Europe PubMed DOI
    37. 2008
  37. Ades,S.E.<br>Regulation by destruction: design of the sigmaE envelope stress response<br>Curr Opin Microbiol (2008) 11, 535-540. PubMed  Europe PubMed DOI
  38. Chandler,J.R. and Dunny,G.M.<br>Characterization of the sequence specificity determinants required for processing and control of sex pheromone by the intramembrane protease Eep and the plasmid-encoded protein PrgY<br>J Bacteriol (2008) 190, 1172-1183. PubMed  Europe PubMed DOI  PMC  EPMC
  39. Denham,E.L., Ward,P.N. and Leigh,J.A.<br>Lipoprotein signal peptides are processed by Lsp and Eep of Streptococcus uberis<br>J Bacteriol (2008) 190, 4641-4647. PubMed  Europe PubMed DOI  PMC  EPMC
  40. Inaba,K., Suzuki,M., Maegawa,K., Akiyama,S., Ito,K. and Akiyama,Y.<br>A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of Escherichia coli<br>J Biol Chem (2008) 283, 35042-35052. PubMed  Europe PubMed DOI
  41. Koide,K., Ito,K. and Akiyama,Y.<br>Substrate recognition and binding by RseP, an Escherichia coli intramembrane protease<br>J Biol Chem (2008) 283, 9562-9570. PubMed  Europe PubMed DOI
    42. 2007
  42. Cezairliyan,B.O. and Sauer,R.T.<br>Inhibition of regulated proteolysis by RseB<br>Proc Natl Acad Sci U S A (2007) 104, 3771-3776. PubMed  Europe PubMed DOI  PMC  EPMC
  43. Chaba,R., Grigorova,I.L., Flynn,J.M., Baker,T.A. and Gross,C.A.<br>Design principles of the proteolytic cascade governing the sigmaE-mediated envelope stress response in Escherichia coli: keys to graded, buffered, and rapid signal transduction<br>Genes Dev (2007) 21, 124-136. PubMed  Europe PubMed DOI  PMC  EPMC
  44. [YEAR:1-1-2007]Hasenbein,S., Merdanovic,M. and Ehrmann,M.<br>Determinants of regulated proteolysis in signal transduction<br>Genes Dev (1-1-2007) 21, 6-10. PubMed  Europe PubMed DOI
  45. King-Lyons,N.D., Smith,K.F. and Connell,T.D.<br>Expression of hurP, a gene encoding a prospective site 2 protease, is essential for heme-dependent induction of bhuR in Bordetella bronchiseptica<br>J Bacteriol (2007) 189, 6266-6275. PubMed  Europe PubMed DOI  PMC  EPMC
  46. Koide,K., Maegawa,S., Ito,K. and Akiyama,Y.<br>Environment of the active site region of RseP, an Escherichia coli regulated intramembrane proteolysis protease, assessed by site-directed cysteine alkylation<br>J Biol Chem (2007) 282, 4553-4560. PubMed  Europe PubMed DOI
  47. Qiu,D., Eisinger,V.M., Rowen,D.W. and Yu,H.D.<br>Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa<br>Proc Natl Acad Sci U S A (2007) 104, 8107-8112. PubMed  Europe PubMed DOI  PMC  EPMC
    48. 2006
  48. [YEAR:2-3-2006]Douchin,V., Bohn,C. and Bouloc,P.<br>Down-regulation of porins by a small RNA bypasses the essentiality of the regulated intramembrane proteolysis protease RseP in Escherichia coli<br>J Biol Chem (2-3-2006) 281, 12253-12259. PubMed  Europe PubMed DOI
    49. 2005
  49. Chen,J.C., Viollier,P.H. and Shapiro,L.<br>A membrane metalloprotease participates in the sequential degradation of a Caulobacter polarity determinant<br>Mol Microbiol (2005) 55, 1085-1103. PubMed  Europe PubMed DOI
  50. [YEAR:27-10-2005]Matson,J.S. and DiRita,V.J.<br>Degradation of the membrane-localized virulence activator TcpP by the YaeL protease in Vibrio cholerae<br>Proc Natl Acad Sci U S A (27-10-2005) 102, 16403-16408. PubMed  Europe PubMed DOI  PMC  EPMC
    51. 2004
  51. [YEAR:10-12-2004]Akiyama,Y., Kanehara,K. and Ito,K.<br>RseP (YaeL), an Escherichia coli RIP protease, cleaves transmembrane sequences<br>EMBO J (10-12-2004) 23, 4434-4442. PubMed  Europe PubMed DOI  PMC  EPMC
  52. Bohn,C., Collier,J. and Bouloc,P.<br>Dispensable PDZ domain of Escherichia coli YaeL essential protease<br>Mol Microbiol (2004) 52, 427-435. PubMed  Europe PubMed DOI
  53. Flynn,J.M., Levchenko,I., Sauer,R.T. and Baker,T.A.<br>Modulating substrate choice: the SspB adaptor delivers a regulator of the extracytoplasmic-stress response to the AAA+ protease ClpXP for degradation<br>Genes Dev (2004) 18, 2292-2301. PubMed  Europe PubMed DOI  PMC  EPMC
  54. [YEAR:1-11-2004]Grigorova,I.L., Chaba,R., Zhong,H.J., Alba,B.M., Rhodius,V., Herman,C. and Gross,C.A.<br>Fine-tuning of the Escherichia coli sigmaE envelope stress response relies on multiple mechanisms to inhibit signal-independent proteolysis of the transmembrane anti-sigma factor, RseA<br>Genes Dev (1-11-2004) 18, 2686-2697. PubMed  Europe PubMed DOI  PMC  EPMC
  55. Rawson,R.B.<br>S2P protease<br>[ISSN:0-12-079610-4] (2004) 2, 985-988.  V
    56. 2003
  56. Kanehara,K., Ito,K. and Akiyama,Y.<br>YaeL proteolysis of RseA is controlled by the PDZ domain of YaeL and a Gln-rich region of RseA<br>EMBO J (2003) 22, 6389-6398. PubMed  Europe PubMed DOI  PMC  EPMC
    57. 2002
  57. [YEAR:15-8-2002]Alba,B.M., Leeds,J.A., Onufryk,C., Lu,C.Z. and Gross,C.A.<br>DegS and YaeL participate sequentially in the cleavage of RseA to activate the sigma(E)-dependent extracytoplasmic stress response<br>Genes Dev (15-8-2002) 16, 2156-2168. PubMed  Europe PubMed DOI  PMC  EPMC
  58. Drew,D., Sjostrand,D., Nilsson,J., Urbig,T., Chin,C.N., de Gier,J.W. and von Heijne,G.<br>Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis<br>Proc Natl Acad Sci U S A (2002) 99, 2690-2695. PubMed  Europe PubMed DOI  PMC  EPMC
  59. [YEAR:15-8-2002]Kanehara,K., Ito,K. and Akiyama,Y.<br>YaeL (EcfE) activates the sigma(E) pathway of stress response through a site-2 cleavage of anti-sigma(E), RseA<br>Genes Dev (15-8-2002) 16, 2147-2155. PubMed  Europe PubMed DOI  PMC  EPMC
    60. 2001
  60. [YEAR:1-11-2001]Dartigalongue,C., Loferer,H. and Raina,S.<br>EcfE, a new essential inner membrane protease: its role in the regulation of heat shock response in Escherichia coli<br>EMBO J (1-11-2001) 20, 5908-5918. PubMed  Europe PubMed DOI  PMC  EPMC
  61. [YEAR:27-12-2001]Kanehara,K., Akiyama,Y. and Ito,K.<br>Characterization of the yaeL gene product and its S2P-protease motifs in Escherichia coli<br>Gene (27-12-2001) 281, 71-79. PubMed  Europe PubMed DOI
    62. 1999
  62. An,F.Y., Sulavik,M.C. and Clewell,D.B.<br>Identification and characterization of a determinant (eep) on the Enterococcus faecalis chromosome that is involved in production of the peptide sex pheromone cAD1<br>J Bacteriol (1999) 181, 5915-5921. PubMed  Europe PubMed  PMC  EPMC

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