Merops Switch to
Inhibitors

Peptidase

Family Clan SEARCHES BLAST
MEROPS SUBMISSIONS

Other
information

Literature for peptidase M23.002: staphylolysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: A Assay, S Structure, P Specificity, E Expression, U Therapeutic, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2024
  1. Keim,K., Bhattacharya,M., Crosby,H.A., Jenul,C., Mills,K., Schurr,M. and Horswill,A.<br>Polymicrobial interactions between Staphylococcus aureus and Pseudomonas aeruginosa promote biofilm formation and persistence in chronic wound infections<br>bioRxiv (2024) PubMed  Europe PubMed DOI  PMC  EPMC
    2. 2021
  2. Rahmani,B., Astani,A., Zarei Jaliani,H., Kheirandish,M.H. and Mosaddegh,A.<br>Evaluation of kinetic stability and anti-staphylococcal activity of recombinant LasA protein produced in Escherichia coli<br>Iran J Basic Med Sci (2021) 24, 851-855. PubMed  Europe PubMed DOI  PMC  EPMC
  3. Vermilyea,D.M., Crocker,A.W., Gifford,A.H. and Hogan,D.A.<br>Calprotectin-mediated zinc chelation inhibits Pseudomonas aeruginosa protease activity in cystic fibrosis sputum<br>J Bacteriol (2021) PubMed  Europe PubMed DOI
    4. 2016
  4. Yang,J., Yu,Y., Tang,B.L., Zhong,S., Shi,M., Xie,B.B., Zhang,X.Y., Zhou,B.C., Zhang,Y.Z. and Chen,X.L.<br>Pilot-scale production and thermostability improvement of the M23 protease pseudoalterin from the deep sea bacterium Pseudoalteromonas sp. CF6-2<br>Molecules (2016) 21 PubMed  Europe PubMed DOI
    5. 2015
  5. Yu,Z.C., Tang,B.L., Zhao,D.L., Pang,X., Qin,Q.L., Zhou,B.C., Zhang,X.Y., Chen,X.L. and Zhang,Y.Z.<br>Development of a cold-adapted Pseudoalteromonas expression system for the Pseudoalteromonas proteins intractable for the Escherichia coli system<br>PLoS ONE (2015) 10, e0137384-e0137384. PubMed  Europe PubMed DOI  PMC  EPMC  E
    6. 2014
  6. Kessler,E. and Safrin,M.<br>Elastinolytic and proteolytic enzymes<br>Methods Mol Biol (2014) 1149, 135-169. PubMed  Europe PubMed DOI  V
  7. Zhao,H.L., Yang,J., Chen,X.L., Su,H.N., Zhang,X.Y., Huang,F., Zhou,B.C. and Xie,B.B.<br>Optimization of fermentation conditions for the production of the M23 protease pseudoalterin by deep-sea Pseudoalteromonas sp. CF6-2 with artery powder as an inducer<br>Molecules (2014) 19, 4779-4790. PubMed  Europe PubMed DOI
    8. 2012
  8. Barequet,I.S., Bourla,N., Pessach,Y.N., Safrin,M., Yankovich,D., Ohman,D.E., Rosner,M. and Kessler,E.<br>Staphylolysin is an effective therapeutic agent for Staphylococcus aureus experimental keratitis<br>Graefes Arch Clin Exp Ophthalmol (2012) 250, 223-229. PubMed  Europe PubMed DOI  U
  9. Kessler,E. and Ohman,D.E.<br>Staphylolysin<br>[ISSN:978-0-12-407743-0] (2012) 3, 1553-1558. DOI
  10. Zhao,H.L., Chen,X.L., Xie,B.B., Zhou,M.Y., Gao,X., Zhang,X.Y., Zhou,B.C., Weiss,A.S. and Zhang,Y.Z.<br>Elastolytic mechanism of a novel M23 metalloprotease pseudoalterin from deep-sea Pseudoalteromonas sp. CF6-2: cleaving not only glycyl bonds in the hydrophobic regions but also peptide bonds in the hydrophilic regions involved in cross-linking<br>J Biol Chem (2012) 287, 39710-39720. PubMed  Europe PubMed DOI  P
    11. 2010
  11. Spencer,J., Murphy,L.M., Conners,R., Sessions,R.B. and Gamblin,S.J.<br>Crystal structure of the LasA virulence factor from Pseudomonas aeruginosa: substrate specificity and mechanism of M23 metallopeptidases<br>J Mol Biol (2010) 396, 908-923. PubMed  Europe PubMed DOI  S
    12. 2009
  12. Barequet,I.S., Habot-Wilner,Z., Mann,O., Safrin,M., Ohman,D.E., Kessler,E. and Rosner,M.<br>Evaluation of Pseudomonas aeruginosa staphylolysin (LasA protease) in the treatment of methicillin-resistant Staphylococcus aureus endophthalmitis in a rat model<br>Graefes Arch Clin Exp Ophthalmol (2009) 247, 913-917. PubMed  Europe PubMed DOI  U
    13. 2007
  13. Elston,C., Wallach,J. and Saulnier,J.<br>New continuous and specific fluorometric assays for Pseudomonas aeruginosa elastase and LasA protease<br>Anal Biochem (2007) 368, 87-94. PubMed  Europe PubMed DOI  A
  14. Grande,K.K., Gustin,J.K., Kessler,E. and Ohman,D.E.<br>Identification of critical residues in the propeptide of LasA protease of Pseudomonas aeruginosa involved in the formation of a stable mature protease<br>J Bacteriol (2007) 189, 3960-3968. PubMed  Europe PubMed DOI  PMC  EPMC
    15. 2004
  15. Barequet,I.S., Ben Simon,G.J., Safrin,M., Ohman,D.E. and Kessler,E.<br>Pseudomonas aeruginosa LasA protease in treatment of experimental staphylococcal keratitis<br>Antimicrobial Agents Chemother (2004) 48, 1681-1687. PubMed  Europe PubMed DOI  PMC  EPMC
  16. Kessler,E. and Ohman,D.E.<br>Staphylolysin<br>[ISSN:0-12-079610-4] (2004) 2, 1001-1003.  V
  17. [YEAR:15-5-2004]Kessler,E., Safrin,M., Blumberg,S. and Ohman,D.E.<br>A continuous spectrophotometric assay for Pseudomonas aeruginosa LasA protease (staphylolysin) using a two-stage enzymatic reaction<br>Anal Biochem (15-5-2004) 328, 225-232. PubMed  Europe PubMed DOI  A
    18. 2002
  18. Hobden,J.A.<br>Pseudomonas aeruginosa proteases and corneal virulence<br>DNA Cell Biol (2002) 21, 391-396. PubMed  Europe PubMed DOI
    19. 2001
  19. Alionte,L.G., Cannon,B.M., White,C.D., Caballero,A.R., O'Callaghan,R.J. and Hobden,J.A.<br>Pseudomonas aeruginosa LasA protease and corneal infections<br>Curr Eye Res (2001) 22, 266-271. PubMed  Europe PubMed DOI
  20. Vessillier,S., Delolme,F., Bernillon,J., Saulnier,J. and Wallach,J.<br>Hydrolysis of glycine-containing elastin pentapeptides by LasA, a metalloelastase from Pseudomonas aeruginosa<br>Eur J Biochem (2001) 268, 1049-1057. PubMed  Europe PubMed DOI
  21. White,C.D., Alionte,L.G., Cannon,B.M., Caballero,A.R., O'Callaghan,R.J. and Hobden,J.A.<br>Corneal virulence of LasA protease-deficient Pseudomonas aeruginosa PAO1<br>Cornea (2001) 20, 643-646. PubMed  Europe PubMed DOI
    22. 2000
  22. Estrellas,P.S., Jr., Alionte,L.G. and Hobden,J.A.<br>A Pseudomonas aeruginosa strain isolated from a contact lens-induced acute red eye (CLARE) is protease-deficient<br>Curr Eye Res (2000) 20, 157-165. PubMed  Europe PubMed
  23. [YEAR:4-2-2000]Park,P.W., Pier,G.B., Preston,M.J., Goldberger,O., Fitzgerald,M.L. and Bernfield,M.<br>Syndecan-1 shedding is enhanced by LasA, a secreted virulence factor of Pseudomonas aeruginosa<br>J Biol Chem (4-2-2000) 275, 3057-3064. PubMed  Europe PubMed DOI
    24. 1998
  24. Braun,P., de Groot,A., Bitter,W. and Tommassen,J.<br>Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa<br>J Bacteriol (1998) 180, 3467-3469. PubMed  Europe PubMed  PMC  EPMC
  25. [YEAR:13-11-1998]Kessler,E., Safrin,M., Gustin,J.K. and Ohman,D.E.<br>Elastase and the LasA protease of Pseudomonas aeruginosa are secreted with their propeptides<br>J Biol Chem (13-11-1998) 273, 30225-30231. PubMed  Europe PubMed DOI
  26. [YEAR:1-9-1998]Park,S. and Galloway,D.R.<br>Pseudomonas aeruginosa LasD processes the inactive LasA precursor to the active protease form<br>Arch Biochem Biophys (1-9-1998) 357, 8-12. PubMed  Europe PubMed DOI
    27. 1997
  27. Kessler,E., Safrin,M., Abrams,W.R., Rosenbloom,J. and Ohman,D.E.<br>Inhibitors and specificity of Pseudomonas aeruginosa lasA<br>J Biol Chem (1997) 272, 9884-9889. PubMed  Europe PubMed DOI
  28. Preston,M.J., Seed,P.C., Toder,D.S., Iglewski,B.H., Ohman,D.E., Gustin,J.K., Goldberg,J.B. and Pier,G.B.<br>Contribution of proteases and LasR to the virulence of Pseudomonas aeruginosa during corneal infections<br>Infect Immun (1997) 65, 3086-3090. PubMed  Europe PubMed  PMC  EPMC
    29. 1996
  29. Gustin,J.K., Kessler,E. and Ohman,D.E.<br>A substitution at His-120 in the lasA protease of Pseudomonas aeruginosa blocks enzymatic activity without affecting propeptide processing or extracellular secretion<br>J Bacteriol (1996) 178, 6608-6617. PubMed  Europe PubMed  PMC  EPMC
    30. 1994
  30. Toder,D.S., Ferrell,S.J., Nezezon,J.L., Rust,L. and Iglewski,B.H.<br>lasA and lasB genes of Pseudomonas aeruginosa: analysis of transcription and gene product activity<br>Infect Immun (1994) 62, 1320-1327. PubMed  Europe PubMed  PMC  EPMC
    31. 1993
  31. Freck-O'Donnell,L.C. and Darzins,A.<br>Pseudomonas aeruginosa lasA gene: Determination of the transcription start point and analysis of the promoter/regulatory region<br>Gene (1993) 129, 113-117. PubMed  Europe PubMed DOI
  32. [YEAR:5-4-1993]Kessler,E., Safrin,M., Olson,J.C. and Ohman,D.E.<br>Secreted LasA of Pseudomonas aeruginosa is a staphylolytic protease<br>J Biol Chem (5-4-1993) 268, 7503-7508. PubMed  Europe PubMed
    33. 1992
  33. Olson,J.C. and Ohman,D.E.<br>Efficient production and processing of elastase and LasA by Pseudomonas aeruginosa require zinc and calcium ions<br>J Bacteriol (1992) 174, 4140-4147. PubMed  Europe PubMed  PMC  EPMC
  34. Peters,J.E., Park,S.J., Darzins,A., Freck,L.C., Saulnier,J.M., Wallach,J.M. and Galloway,D.R.<br>Further studies on Pseudomonas aeruginosa LasA: analysis of specificity<br>Mol Microbiol (1992) 6, 1155-1162. PubMed  Europe PubMed DOI
    35. 1991
  35. Toder,D.S., Gambello,M.J. and Iglewski,B.H.<br>Pseudomonas aeruginosa LasA: a second elastase under the transcriptional control of lasR<br>Mol Microbiol (1991) 5, 2003-2010. PubMed  Europe PubMed
    36. 1990
  36. [YEAR:11-11-1990]Darzins,A., Peters,J.E. and Galloway,D.R.<br>Revised nucleotide sequence of the lasA gene from Pseudomonas aeruginosa PAO1<br>Nucleic Acids Res (11-11-1990) 18, 6444-6444. PubMed  Europe PubMed  PMC  EPMC
  37. Peters,J.E. and Galloway,D.R.<br>Purification and characterization of an active fragment of the LasA protein from Pseudomonas aeruginosa: enhancement of elastase activity<br>J Bacteriol (1990) 172, 2236-2240. PubMed  Europe PubMed  PMC  EPMC
    38. 1989
  38. Brito,N., Falcon,M.A., Carnicero,A., Gutierrez-Navarro,A.M. and Mansito,T.B.<br>Purification and peptidase activity of a bacteriolytic extracellular enzyme from Pseudomonas aeruginosa<br>Res Microbiol (1989) 140, 125-137. PubMed  Europe PubMed
    39. 1988
  39. Schad,P.A. and Iglewski,B.H.<br>Nucleotide sequence and expression in Escherichia coli of the Pseudomonas aeruginosa lasA gene<br>J Bacteriol (1988) 170, 2784-2789. PubMed  Europe PubMed  PMC  EPMC
    40. 1987
  40. Goldberg,J.B. and Ohman,D.E.<br>Activation of an elastase precursor by the lasA gene product of Pseudomonas aeruginosa<br>J Bacteriol (1987) 169, 4532-4539. PubMed  Europe PubMed  PMC  EPMC
  41. Goldberg,J.B. and Ohman,D.E.<br>Cloning and transcriptional regulation of the elastase lasA gene in mucoid and nonmucoid Pseudomonas aeruginosa<br>J Bacteriol (1987) 169, 1349-1351. PubMed  Europe PubMed  PMC  EPMC
    42. 1985
  42. Perestelo,F.R., Blanco,M.T., Gutierrez-Navarro,A.M. and Falcon,M.A.<br>Growth inhibition of Staphylococcus aureus by a staphylolytic enzyme from Pseudomonas aeruginosa<br>Microbios Lett (1985) 30, 85-94.
    43. 1969
  43. Lache,M., Hearn,W.R., Zyskind,J.W., Tipper,D.J. and Strominger,J.L.<br>Specificity of a bacteriolytic enzyme from Pseudomonas aeruginosa<br>J Bacteriol (1969) 100, 254-259. PubMed  Europe PubMed  PMC  EPMC

feedback
Page created 8-September-2023

EMBL-EBI logo

© 2025 EBI
Terms of use