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Literature for peptidase C09.001: sindbis virus-type nsP2 peptidase

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, A Assay, P Specificity, I Inhibitor, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Dansana,J., Purohit,P., Panda,M. and Meher,B.R.
    Recent advances in phytocompounds as potential Chikungunya virus non-structural protein 2 protease antagonists: A systematic review
    Phytomedicine136, 156359-156359. PubMed  Europe PubMed DOI
  2. Ghoshal,A., Tse,E.G., Hossain,M.A., Asressu,K.H., Merten,E.M., Sears,J.D., Howell,S., Perveen,S., Burdick,J., Morales,N.L., Martinez,S.A., Law,I., Davenport,B.J., Morrison,T.E., Streblow,Z.J., Streblow,D.N., Mordant,A.L., Webb,T.S., Cabrera,A., Herring,L.E., Arrowsmith,C.H., Pearce,K.H., Moorman,N.J., Heise,M.T., Counago,R.M., Brown,P.J. and Willson,T.M.
    A covalent chemical probe for Chikungunya nsP2 cysteine protease with antialphaviral activity and proteome-wide selectivity
    Sci Rep15, 7264-7264. PubMed  Europe PubMed DOI
  3. He,J., Illingworth,H., Ullrich,S., Ghosh,P., Ton,J., Jackson,C.J. and Nitsche,C.
    Synthesis, screening and validation of cysteine-reactive fragments as chikungunya virus protease inhibitors
    Bioorg Med Chem Lett122, 130176-130176. PubMed  Europe PubMed DOI
  4. Makhaik,S., Rut,W., Choudhary,S., Upadhyay,T., Hao,C., Westberg,M., Bobst,C., Yoo,E., Fejzo,J., Lin,M.Z., Bogyo,M., Thompson,P., Drag,M. and Hardy,J.A.
    A robust fluorogenic substrate for chikungunya virus protease (nsP2) activity
    Protein Sci34, e70069-e70069. PubMed  Europe PubMed DOI
  5. Mastalipour,M., Gering,I., Coronado,M.A., Gonzalez,J.E.H., Willbold,D. and Eberle,R.J.
    Novel peptide inhibitor for the Chikungunya virus nsP2 protease: Identification and characterization
    Curr Res Microb Sci8, 100376-100376. PubMed  Europe PubMed DOI  I
    6. 2024
  6. Ghoshal,A., Asressu,K.H., Hossain,M.A., Brown,P.J., Nandakumar,M., Vala,A., Merten,E.M., Sears,J.D., Law,I., Burdick,J.E., Morales,N.L., Perveen,S., Pearce,K.H., Popov,K.I., Moorman,N.J., Heise,M.T. and Willson,T.M.
    Structure Activity of beta-Amidomethyl Vinyl Sulfones as Covalent Inhibitors of Chikungunya nsP2 Cysteine Protease with Antialphavirus Activity
    J Med Chem PubMed  Europe PubMed DOI  I
  7. Ghoshal,A., Tse,E.G., Hossain,M.A., Asressu,K.H., Merten,E.M., Sears,J.D., Howell,S., Perveen,S., Burdick,J., Morales,N.L., Martinez,S.A., Law,I., Davenport,B.J., Morrison,T.E., Streblow,Z.J., Streblow,D.N., Mordant,A.L., Webb,T.S., Cabrera,A., Herring,L.E., Arrowsmith,C.H., Pearce,K.H., Moorman,N.J., Heise,M.T., Counago,R.M., Brown,P.J. and Willson,T.M.
    A Covalent Chemical Probe for Chikungunya nsP2 Cysteine Protease with Antialphaviral Activity and Proteome-wide Selectivity
    Res Sq PubMed  Europe PubMed DOI
  8. Merten,E.M., Sears,J.D., Leisner,T.M., Hardy,P.B., Ghoshal,A., Hossain,M.A., Asressu,K.H., Brown,P.J., Tse,E.G., Stashko,M.A., Li,K., Norris-Drouin,J.L., Herring,L.E., Mordant,A.L., Webb,T.S., Mills,C.A., Barker,N.K., Streblow,Z.J., Perveen,S., Arrowsmith,C.H., Counago,R.M., Arnold,J.J., Cameron,C.E., Streblow,D.N., Moorman,N.J., Heise,M.T., Willson,T.M., Popov,K.I. and Pearce,K.H.
    Identification of a cell-active chikungunya virus nsP2 protease inhibitor using a covalent fragment-based screening approach
    Proc Natl Acad Sci U S A121, e2409166121-e2409166121. PubMed  Europe PubMed DOI  I
  9. Merten,E.M., Sears,J.D., Leisner,T.M., Hardy,P.B., Ghoshal,A., Hossain,M.A., Asressu,K.H., Brown,P.J., Stashko,M.A., Herring,L.E., Mordant,A.L., Webb,T.S., Mills,C.A., Barker,N.K., Arnold,J.J., Cameron,C.E., Streblow,D.N., Moorman,N.J., Heise,M., Willson,T.M., Popov,K.I. and Pearce,K.H.
    Discovery of a cell-active chikungunya virus nsP2 protease inhibitor using a covalent fragment-based screening approach
    bioRxiv PubMed  Europe PubMed DOI  I
  10. Metibemu,D.S., Adeyinka,O.S., Falode,J., Hampton,T., Crown,O., Ojobor,J.C., Narayanan,A., Julander,J. and Ogungbe,I.V.
    Inhibitor of the non-structural protein 2 protease shows promising efficacy in mouse models of chikungunya
    Eur J Med Chem278, 116808-116808. PubMed  Europe PubMed DOI  I
  11. Rabelo,V.W., Sanchez-Nunez,M.L., Correa-Amorim,L.S., Kuhn,R.J., Abreu,P.A. and Paixao,I.C.N.P.
    In Silico Drug Repurposing Uncovered the Antiviral Potential of the Antiparasitic Drug Oxibendazole Against the Chikungunya Virus
    ACS Omega9, 27632-27642. PubMed  Europe PubMed DOI
  12. Sharma,K.B., Subramani,C., Ganesh,K., Sharma,A., Basu,B., Balyan,S., Sharma,G., Ka,S., Deb,A., Srivastava,M., Chugh,S., Sehrawat,S., Bharadwaj,K., Rout,A., Sahoo,P.K., Saurav,S., Motiani,R.K., Singh,R., Jain,D., Asthana,S., Wadhwa,R. and Vrati,S.
    Withaferin A inhibits Chikungunya virus nsP2 protease and shows antiviral activity in the cell culture and mouse model of virus infection
    PLoS Pathog20, e1012816-e1012816. PubMed  Europe PubMed DOI  I
    13. 2023
  13. Souza,B.G., Choudhary,S., Vilela,G.G., Passos,G.F.S., Costa,C.A.C.B., Freitas,J.D., Coelho,G.L., Brandao,J.A., Anderson,L., Bassi,E.J., Araujo-Junior,J.X., Tomar,S. and Silva-Junior,E.F.D.
    Design, synthesis, antiviral evaluation, and In silico studies of acrylamides targeting nsP2 from Chikungunya virus
    Eur J Med Chem258, 115572-115572. PubMed  Europe PubMed DOI  I
    14. 2022
  14. De,S., Ghosh,S., Keshry,S.S., Mahish,C., Mohapatra,C., Guru,A., Mamidi,P., Datey,A., Pani,S.S., Vasudevan,D., Beuria,T.K., Chattopadhyay,S., Subudhi,B.B. and Chattopadhyay,S.
    MBZM-N-IBT, a Novel Small Molecule, Restricts Chikungunya Virus Infection by Targeting nsP2 Protease Activity In Vitro, In Vivo, and Ex Vivo
    Antimicrob Agents Chemother66, e0046322-e0046322. PubMed  Europe PubMed DOI
  15. Laurent,T., Kumar,P., Liese,S., Zare,F., Jonasson,M., Carlson,A. and Carlson,L.A.
    Architecture of the chikungunya virus replication organelle
    elife11, PubMed  Europe PubMed DOI
  16. Rabelo,V.W., de Palmer Paixao,I.C.N. and Abreu,P.A.
    Structural insights into the inhibition of the nsP2 protease from Chikungunya virus by molecular modeling approaches
    J Mol Model28, 311-311. PubMed  Europe PubMed DOI
    17. 2021
  17. Ivanova,L., Rausalu,K., Zusinaite,E., Tammiku-Taul,J., Merits,A. and Karelson,M.
    1,3-Thiazolbenzamide Derivatives as Chikungunya Virus nsP2 Protease Inhibitors
    ACS Omega6, 5786-5794. PubMed  Europe PubMed DOI  I
  18. Ivanova,L., Rausalu,K., Oseka,M., Kananovich,D.G., Zusinaite,E., Tammiku-Taul,J., Lopp,M., Merits,A. and Karelson,M.
    Novel Analogues of the Chikungunya Virus Protease Inhibitor: Molecular Design, Synthesis, and Biological Evaluation
    ACS Omega6, 10884-10896. PubMed  Europe PubMed DOI
    19. 2020
  19. Fazal,F., Anwar,T., Waheed,Y. and Parvaiz,F.
    Prediction of promiscuous epitopes in NSP2 of Chikungunya virus: An in-silico approach
    Trop Biomed37, 566-577. PubMed  Europe PubMed DOI
  20. Law,Y.S., Wang,S., Tan,Y.B., Shih,O., Utt,A., Goh,W.Y., Lian,B.J., Chen,M.W., Jeng,U.S., Merits,A. and Luo,D.
    Inter-domain Flexibility of Chikungunya Virus nsP2 Helicase-Protease Differentially Influences Viral RNA Replication and Infectivity
    J Virol PubMed  Europe PubMed DOI
    21. 2019
  21. Kumar,P., Kumar,D. and Giri,R.
    Targeting the nsp2 cysteine protease of Chikungunya virus using FDA approved library and selected cysteine protease inhibitors
    Pathogens8, PubMed  Europe PubMed DOI
    22. 2018
  22. Lulla,V., Karo-Astover,L., Rausalu,K., Saul,S., Merits,A. and Lulla,A.
    Timeliness of proteolytic events is prerequisite for efficient functioning of the alphaviral replicase
    J Virol92, e00151-18-e00151-18. PubMed  Europe PubMed DOI
  23. Narwal,M., Singh,H., Pratap,S., Malik,A., Kuhn,R.J., Kumar,P. and Tomar,S.
    Crystal structure of chikungunya virus nsP2 cysteine protease reveals a putative flexible loop blocking its active site
    Int J Biol Macromol116, 451-462. PubMed  Europe PubMed DOI  A  S
  24. Ramphan,S., Khongwichit,S., Saisawang,C., Kovanich,D., Ketterman,A.J., Ubol,S., Auewarakul,P., Roytrakul,S., Smith,D.R. and Kuadkitkan,A.
    Ubiquitin-conjugating enzyme E2 L3 is downregulated by the Chikungunya virus nsP2 protease
    Proteomics Clin Appl12, e1700020-e1700020. PubMed  Europe PubMed DOI
  25. Saha,A., Acharya,B.N., Priya,R., Tripathi,N.K., Shrivastava,A., Rao,M.K., Kesari,P., Narwal,M., Tomar,S., Bhagyawant,S.S., Parida,M. and Dash,P.K.
    Development of nsP2 protease based cell free high throughput screening assay for evaluation of inhibitors against emerging Chikungunya virus
    Sci Rep8, 10831-10831. PubMed  Europe PubMed DOI  A  I
  26. Singh,H., Mudgal,R., Narwal,M., Kaur,R., Singh,V.A., Malik,A., Chaudhary,M. and Tomar,S.
    Chikungunya virus inhibition by peptidomimetic inhibitors targeting virus-specific cysteine protease
    Biochimie149, 51-61. PubMed  Europe PubMed DOI  A
    27. 2017
  27. Dhindwal,S., Kesari,P., Singh,H., Kumar,P. and Tomar,S.
    Conformer and pharmacophore based identification of peptidomimetic inhibitors of Chikungunya virus nsP2 protease
    J Biomol Struct Dyn35, 3522-3539. PubMed  Europe PubMed DOI  I
  28. Ramakrishnan,C., Kutumbarao,N.H.V., Suhitha,S. and Velmurugan,D.
    Structure-function relationship of Chikungunya nsP2 protease: a comparative study with papain
    Chem Biol Drug Des89, 772-782. PubMed  Europe PubMed DOI  S
  29. Saisawang,C., Kuadkitkan,A., Smith,D.R., Ubol,S. and Ketterman,A.J.
    Glutathionylation of chikungunya nsP2 protein affects protease activity
    Biochim Biophys Acta1861, 106-111. PubMed  Europe PubMed DOI
    30. 2016
  30. Byler,K.G., Collins,J.T., Ogungbe,I.V. and Setzer,W.N.
    Alphavirus protease inhibitors from natural sources: a homology modeling and molecular docking investigation
    Comput Biol Chem64, 163-184. PubMed  Europe PubMed DOI  I
  31. Das,P.K., Puusepp,L., Varghese,F.S., Utt,A., Ahola,T., Kananovich,D.G., Lopp,M., Merits,A. and Karelson,M.
    Design and validation of novel chikungunya virus protease inhibitors
    Antimicrob Agents Chemother60, 7382-7395. PubMed  Europe PubMed DOI  I
  32. Rausalu,K., Utt,A., Quirin,T., Varghese,F.S., Zusinaite,E., Das,P.K., Ahola,T. and Merits,A.
    Chikungunya virus infectivity, RNA replication and non-structural polyprotein processing depend on the nsP2 protease's active site cysteine residue
    Sci Rep6, 37124-37124. PubMed  Europe PubMed DOI  P
    33. 2015
  33. Agarwal,T., Asthana,S. and Bissoyi,A.
    Molecular modeling and docking study to elucidate novel Chikungunya virus nsP2 protease inhibitors
    Indian J Pharm Sci77, 453-460. PubMed  Europe PubMed  I
  34. El-Labbad,E.M., Ismail,M.A., Abou Ei Ella,D.A., Ahmed,M., Wang,F., Barakat,K.H. and Abouzid,K.A.
    Discovery of novel peptidomimetics as irreversible CHIKV nsP2 protease inhibitors using quantum mechanical-based ligand descriptors
    Chem Biol Drug Des86, 1518-1527. PubMed  Europe PubMed DOI  I
  35. Jadav,S.S., Sinha,B.N., Hilgenfeld,R., Pastorino,B., de Lamballerie,X. and Jayaprakash,V.
    Thiazolidone derivatives as inhibitors of chikungunya virus
    Eur J Med Chem89, 172-178. PubMed  Europe PubMed DOI  I
  36. Nguyen,P.T., Yu,H. and Keller,P.A.
    Identification of chikungunya virus nsP2 protease inhibitors using structure-base approaches
    J Mol Graph Model57C, 1-8. PubMed  Europe PubMed DOI  I
  37. Saisawang,C., Saitornuang,S., Sillapee,P., Ubol,S., Smith,D.R. and Ketterman,A.J.
    Chikungunya nsP2 protease is not a papain-like cysteine protease and the catalytic dyad cysteine is interchangeable with a proximal serine
    Sci Rep5, 17125-17125. PubMed  Europe PubMed DOI
  38. Saisawang,C., Sillapee,P., Sinsirimongkol,K., Ubol,S., Smith,D.R. and Ketterman,A.J.
    Full length and protease domain activity of chikungunya virus nsP2 differ from other alphavirus nsP2 proteases in recognition of small peptide substrates
    Biosci Rep35, PubMed  Europe PubMed DOI  P
    39. 2013
  39. Bassetto,M., De Burghgraeve,T., Delang,L., Massarotti,A., Coluccia,A., Zonta,N., Gatti,V., Colombano,G., Sorba,G., Silvestri,R., Tron,G.C., Neyts,J., Leyssen,P. and Brancale,A.
    Computer-aided identification, design and synthesis of a novel series of compounds with selective antiviral activity against chikungunya virus
    Antiviral Res98, 12-18. PubMed  Europe PubMed DOI  I
  40. Lulla,V., Karo-Astover,L., Rausalu,K., Merits,A. and Lulla,A.
    Presentation overrides specificity: probing the plasticity of alphaviral proteolytic activity through mutational analysis
    J Virol87, 10207-10220. PubMed  Europe PubMed DOI  P
    41. 2012
  41. Bora,L.
    Homology modeling and docking to potential novel inhibitor for Chikungunya (37997) protein nsP2 protease
    J Proteomics Bioinformatics5, 54-59. DOI
  42. Lulla,A., Lulla,V. and Merits,A.
    Macromolecular assembly-driven processing of the 2/3 cleavage site in the alphavirus replicase polyprotein
    J Virol86, 553-565. PubMed  Europe PubMed DOI
  43. Shin,G., Yost,S.A., Miller,M.T., Elrod,E.J., Grakoui,A. and Marcotrigiano,J.
    Structural and functional insights into alphavirus polyprotein processing and pathogenesis
    Proc Natl Acad Sci U S A109, 16534-16539. PubMed  Europe PubMed DOI
  44. Singh,K., Kirubakaran,P., Nagarajan,S., Sakkiah,S., Muthusamy,K., Velmurgan,D. and Jeyakanthan,J.
    Homology modeling, molecular dynamics, e-pharmacophore mapping and docking study of Chikungunya virus nsP2 protease
    J Mol Model18, 39-51. PubMed  Europe PubMed DOI  S
  45. Strauss,E.G. and Strauss,J.H.
    Sindbis virus nsP2 endopeptidase
    [ISSN:978-0-12-407743-0]3, 2234-2236. DOI
    46. 2009
  46. Zhang,D., Tozser,J. and Waugh,D.S.
    Molecular cloning, overproduction, purification and biochemical characterization of the p39 nsp2 protease domains encoded by three alphaviruses
    Protein Expr Purif64, 89-97. PubMed  Europe PubMed DOI  E
    47. 2008
  47. Pastorino,B.A., Peyrefitte,C.N., Almeras,L., Grandadam,M., Rolland,D., Tolou,H.J. and Bessaud,M.
    Expression and biochemical characterization of nsP2 cysteine protease of Chikungunya virus
    Virus Res131, 293-298. PubMed  Europe PubMed DOI
    48. 2006
  48. [YEAR:20-2-2006]Golubtsov,A., Kaariainen,L. and Caldentey,J.
    Characterization of the cysteine protease domain of Semliki Forest virus replicase protein nsP2 by in vitro mutagenesis
    FEBS Lett580, 1502-1508. PubMed  Europe PubMed DOI  I
  49. Lulla,A., Lulla,V., Tints,K., Ahola,T. and Merits,A.
    Molecular determinants of substrate specificity for Semliki Forest virus nonstructural protease
    J Virol80, 5413-5422. PubMed  Europe PubMed DOI  P
    50. 2004
  50. Strauss,E.G. and Strauss,J.H.
    Sindbis virus nsP2 endopeptidase
    [ISSN:0-12-079610-4]2, 1269-1271.  V
    51. 2003
  51. [YEAR:24-10-2003]Vasiljeva,L., Merits,A., Golubtsov,A., Sizemskaja,V., Kaariainen,L. and Ahola,T.
    Regulation of the sequential processing of Semliki Forest virus replicase polyprotein
    J Biol Chem278, 41636-41645. PubMed  Europe PubMed DOI
    52. 2001
  52. Merits,A., Vasiljeva,L., Ahola,T., Kaariainen,L. and Auvinen,P.
    Proteolytic processing of Semliki Forest virus-specific non-structural polyprotein by nsP2 protease
    J Gen Virol82, 765-773. PubMed  Europe PubMed
  53. [YEAR:17-8-2001]Vasiljeva,L., Valmu,L., Kaariainen,L. and Merits,A.
    Site-specific protease activity of the carboxyl-terminal domain of Semliki Forest virus replicase protein nsP2
    J Biol Chem276, 30786-30793. PubMed  Europe PubMed DOI
    54. 1999
  54. Ten Dam,E., Flint,M. and Ryan,M.D.
    Virus-encoded proteinases of the Togaviridae
    J Gen Virol80, 1879-1888. PubMed  Europe PubMed
    55. 1995
  55. Rumenapf,T., Strauss,E.G. and Strauss,J.H.
    Aura virus is a New World representative of Sindbis-like viruses
    Virology208, 621-633. PubMed  Europe PubMed DOI
    56. 1994
  56. Lemm,J.A., Rumenapf,T., Strauss,E.G., Strauss,J.H. and Rice,C.M.
    Polypeptide requirements for assembly of functional Sindbis virus replication complexes: a model for the temporal regulation of minus- and plus-strand RNA synthesis
    EMBO J13, 2925-2934. PubMed  Europe PubMed
    57. 1992
  57. Strauss,E.G., de Groot,R.J., Levinson,R. and Strauss,J.H.
    Identification of the active site residues in the nsP2 proteinase of sindbis virus
    Virology191, 932-940. PubMed  Europe PubMed DOI
    58. 1991
  58. Gorbalenya,A.E., Koonin,E.V. and Lai,M.M.C.
    Putative papain-related thiol proteases of positive-strand RNA viruses. Identification of rubi- and aphthovirus proteases and delineation of a novel conserved domain associated with proteases of rubi-, alpha- and coronaviruses
    FEBS Lett288, 201-205. PubMed  Europe PubMed DOI
    59. 1990
  59. de Groot,R.J., Hardy,W.R., Shirako,Y. and Strauss,J.H.
    Cleavage-site preferences of sindbis virus polyproteins containing the non-structural proteinase. Evidence for temporal regulation of polyprotein processing in vivo
    EMBO J9, 2631-2638. PubMed  Europe PubMed  P
  60. Hardy,W.R., Hahn,Y.S., de Groot,R.J., Strauss,E.G. and Strauss,J.H.
    Synthesis and processing of the nonstructural polyproteins of several temperature-sensitive mutants of Sindbis virus
    Virology177, 199-208. PubMed  Europe PubMed DOI
    61. 1989
  61. Ding,M.X. and Schlesinger,M.J.
    Evidence that sindbis virus NSP2 is an autoprotease which processes the virus nonstructural polyprotein
    Virology171, 280-284. PubMed  Europe PubMed
  62. Hardy,W.R. and Strauss,J.H.
    Processing the nonstructural polyproteins of sindbis virus: nonstructural proteinase is in the C-terminal half of nsP2 and functions both in cis and in trans
    J Virol63, 4653-4664. PubMed  Europe PubMed
    63. 1988
  63. Hardy,W.R. and Strauss,J.H.
    Processing the nonstructural polyproteins of Sindbis virus: study of the kinetics in vivo by using monospecific antibodies
    J Virol62, 998-1007. PubMed  Europe PubMed
    64. 1981
  64. Rice,C.M. and Strauss,J.H.
    Nucleotide sequence of the 26S mRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins
    Proc Natl Acad Sci U S A78, 2062-2066. PubMed  Europe PubMed

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