Literature for peptidase A01.020: phytepsin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: A Assay, S Structure, I Inhibitor, P Specificity, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

2021

Ibanez,I.L., Munoz,F.F., Zoppi,J., Abaurrea,R.A., Scandogliero,E.A., Duran,H. and Guevara,M.G. In vivo tumor growth inhibition by Solanum tuberosum aspartic protease 3 (StAP3) treatment Bioorg Med Chem Lett (2021) 41, 127959-127959. PubMed Europe PubMed DOI

2020

Folgado,A., Pires,A.S., Figueiredo,A.C., Pimentel,C. and Abranches,R. Toward alternative sources of milk coagulants for cheese manufacturing: establishment of hairy roots culture and protease characterization from Cynara cardunculus L Plant Cell Rep (2020) 39, 89-100. PubMed Europe PubMed DOI
Folgado,A. and Abranches,R. Plant aspartic proteases for industrial applications: thistle get better Plants (Basel) (2020) 9 PubMed Europe PubMed DOI
Sebastian,D., Fernando,F.D., Raul,D.G. and Gabriela,G.M. Overexpression of Arabidopsis aspartic protease APA1 gene confers drought tolerance Plant Sci (2020) 292, 110406-110406. PubMed Europe PubMed DOI

2019

Bueno-Gavila,E., Abellan,A., Giron-Rodriguez,F., Cayuela,J.M., Salazar,E., Gomez,R. and Tejada,L. Bioactivity of hydrolysates obtained from bovine casein using artichoke (Cynara scolymus L.) proteases J Dairy Sci (2019) 102, 10711-10723. PubMed Europe PubMed DOI
Wu,H., Castanheira,P., Faro,C. and Tang,J. Cardosin A endocytosis mediated by integrin leads to lysosome leakage and apoptosis of epithelial cells Proteins (2019) 87, 502-511. PubMed Europe PubMed DOI

2018

Almeida,C.M. and Simoes,I. Cardoon-based rennets for cheese production Appl Microbiol Biotechnol (2018) 102, 4675-4686. PubMed Europe PubMed DOI
Chen,Y., Chen,Y., Zhao,L., Kong,X., Yang,Z. and Hua,Y. A two-chain aspartic protease present in seeds with high affinity for peanut oil bodies Food Chem (2018) 241, 443-451. PubMed Europe PubMed DOI
Diaz,M.E., Rocha,G.F., Kise,F., Rosso,A.M., Guevara,M.G. and Parisi,M.G. Antimicrobial activity of an aspartic protease from Salpichroa origanifolia fruits Lett Appl Microbiol (2018) 67, 168-174. PubMed Europe PubMed DOI

2017

Almeida,C.M., Manso,J.A., Figueiredo,A.C., Antunes,L., Cruz,R., Manadas,B., Bur,D., Pereira,P.J.B., Faro,C. and Simoes,I. Functional and structural characterization of synthetic cardosin B-derived rennet Appl Microbiol Biotechnol (2017) 101, 6951-6968. PubMed Europe PubMed DOI E
Ben Amira,A., Bauwens,J., de Pauw,E., Besbes,S., Attia,H., Francis,F. and Blecker,C. Identification of proteins from wild cardoon flowers (Cynara cardunculus L.) by a proteomic approach J Chem Biol (2017) 10, 25-33. PubMed Europe PubMed DOI PMC EPMC
Bryksa,B.C. and Yada,R.Y. Protein structure insights into the bilayer interactions of the saposin-like domain of Solanum tuberosum aspartic protease Sci Rep (2017) 7, 16911-16911. PubMed Europe PubMed DOI PMC EPMC
Sampaio,P.N., Sales,K.C., Rosa,F.O., Lopes,M.B. and Calado,C.R. High-throughput FTIR-based bioprocess analysis of recombinant cyprosin production J Ind Microbiol Biotechnol (2017) 44, 49-61. PubMed Europe PubMed DOI E

2016

Esposito,M., DiPierro,P., Dejonghe,W., Mariniello,L. and Porta,R. Enzymatic milk clotting activity in artichoke (Cynara scolymus) leaves and alpine thistle (Carduus defloratus) flowers. Immobilization of alpine thistle aspartic protease Food Chem (2016) 204, 115-121. PubMed Europe PubMed DOI

2015

Almeida,C.M., Gomes,D., Faro,C. and Simoes,I. Engineering a cardosin B-derived rennet for sheep and goat cheese manufacture Appl Microbiol Biotechnol (2015) 99, 269-281. PubMed Europe PubMed DOI
Cimino,C.V., Colombo,M.L., Liggieri,C., Bruno,M. and Vairo-Cavalli,S. Partial molecular characterization of Arctium minus aspartylendopeptidase and preparation of bioactive peptides by whey protein hydrolysis J Med Food (2015) 18, 856-864. PubMed Europe PubMed DOI
Darabi,M. and Seddigh,S. Bioinformatic characterization of aspartic protease (AP) enzyme in seed plants Plant Syst Evol (2015) 301, 2399-2417. DOI
Kumar,S., Briguglio,J.S. and Turkewitz,A.P. Secretion of polypeptide crystals from Tetrahymena thermophila secretory organelles (mucocysts) depends on processing by a cysteine cathepsin, Cth4p Eukaryot Cell (2015) 14, 817-833. PubMed Europe PubMed DOI
Rocha,G.F., Obregon,W.D., Munoz,F., Guevara,M.G., Fernandez,G., Rosso,A.M. and Parisi,M.G. Isolation and characterization of an aspartic protease from Salpichroa origanifolia fruits Protein Pept Lett (2015) 22, 379-390. PubMed Europe PubMed P

2014

Curto,P., Lufrano,D., Pinto,C., Custodio,V., Gomes,A.C., Trejo,S.A., Bakas,L., Vairo-Cavalli,S., Faro,C. and Simoes,I. Establishing the yeast Kluyveromyces lactis as an expression host for production of the saposin-like domain of the aspartic protease cirsin Appl Environ Microbiol (2014) 80, 86-96. PubMed Europe PubMed DOI E
De Moura,D.C., Bryksa,B.C. and Yada,R.Y. In silico insights into protein-protein interactions and folding dynamics of the saposin-like domain of Solanum tuberosum aspartic protease PLoS ONE (2014) 9, e104315-e104315. PubMed Europe PubMed DOI PMC EPMC
Llorente,B.E., Obregon,W.D., Aviles,F.X., Caffini,N.O. and Vairo-Cavalli,S. Use of artichoke (Cynara scolymus) flower extract as a substitute for bovine rennet in the manufacture of Gouda-type cheese: characterization of aspartic proteases Food Chem (2014) 159, 55-63. PubMed Europe PubMed DOI
Sampaio,P.N., Sales,K.C., Rosa,F.O., Lopes,M.B. and Calado,C.R. In situ near infrared spectroscopy monitoring of cyprosin production by recombinant Saccharomyces cerevisiae strains J Biotechnol (2014) 188C, 148-157. PubMed Europe PubMed DOI E
Sampaio,P.N., Pais,M.S. and Fonseca,L.P. A novel fed-batch based strategy for enhancing cell-density and recombinant cyprosin B production in bioreactors Bioprocess Biosyst Eng (2014) 37, 2515-2527. PubMed Europe PubMed DOI
Wang,C., Rao,H., Zhou,Z. and Zhang,Y. Mutagenic breeding of Quambalaria cyanescens strain for the production of milk-clotting enzymes Pak J Pharm Sci (2014) 27, 649-657. PubMed Europe PubMed

2013

Ordiales,E., Benito,M.J., Martin,A., Fernandez,M., Hernandez,A. and de Guia Cordoba,M. Proteolytic effect of Cynara cardunculus rennet for use in the elaboration of 'Torta del Casar' cheese J Dairy Res (2013) 80, 429-438. PubMed Europe PubMed DOI
Raimbault,A.K., Zuily-Fodil,Y., Soler,A. and Cruz de Carvalho,M.H. A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): Cloning, characterization and relation to postharvest chilling stress resistance J Plant Physiol (2013) 170, 1536-1540. PubMed Europe PubMed DOI
Vairo Cavalli,S., Lufrano,D., Colombo,M.L. and Priolo,N. Properties and applications of phytepsins from thistle flowers Phytochemistry (2013) 92, 16-32. PubMed Europe PubMed DOI

2012

Fraga,A.S., Esteves,A.C., Micaelo,N., Cruz,P.F., Brito,R.M., Nutley,M., Cooper,A., Barros,M.M. and Pires,E.M. Functional and conformational changes in the aspartic protease cardosin A induced by TFE Int J Biol Macromol (2012) 50, 323-330. PubMed Europe PubMed DOI
Garcia,V., Rovira,S., Teruel,R., Boutoial,K., Rodriguez,J., Roa,I. and Lopez,M.B. Effect of vegetable coagulant, microbial coagulant and calf rennet on physicochemical, proteolysis, sensory and texture profiles of fresh goats cheese Dairy Sci Technol (2012) 92, 691-707. DOI
Kervinen,J. and Wlodawer,A. Phytepsin [ISSN:978-0-12-407744-7] (2012) 3, 118-124. DOI
Lufrano,D., Faro,R., Castanheira,P., Parisi,G., Verissimo,P., Vairo-Cavalli,S., Simoes,I. and Faro,C. Molecular cloning and characterization of procirsin, an active aspartic protease precursor from Cirsium vulgare (Asteraceae) Phytochemistry (2012) 81, 7-18. PubMed Europe PubMed DOI

2011

Bryksa,B.C., Bhaumik,P., Magracheva,E., De Moura,D.C., Kurylowicz,M., Zdanov,A., Dutcher,J.R., Wlodawer,A. and Yada,R.Y. Structure and mechanism of the saposin-like domain of a plant aspartic protease J Biol Chem (2011) 286, 28265-28275. PubMed Europe PubMed DOI S
da Costa,D.S., Pereira,S. and Pissarra,J. The heterologous systems in the study of cardosin B trafficking pathways Plant Signal Behav (2011) 6, 895-897. PubMed Europe PubMed DOI
Munoz,F., Palomares-Jerez,M.F., Daleo,G., Villalain,J. and Guevara,M.G. Cholesterol and membrane phospholipid compositions modulate the leakage capacity of the swaposin domain from a potato aspartic protease (StAsp-PSI) Biochim Biophys Acta (2011) 1811, 1038-1044. PubMed Europe PubMed DOI
Sampaio,P.N., Sousa,L., Calado,C.R., Pais,M.S. and Fonseca,L.P. Use of chemometrics in the selection of a Saccharomyces cerevisiae expression system for recombinant cyprosin B production Biotechnol Lett (2011) 33, 2111-2119. PubMed Europe PubMed DOI E

2010

Contour-Ansel,D., Torres-Franklin,M.L., Zuily-Fodil,Y. and de Carvalho,M.H. An aspartic acid protease from common bean is expressed 'on call' during water stress and early recovery J Plant Physiol (2010) 167, 1606-1612. PubMed Europe PubMed DOI
da Costa,D.S., Pereira,S., Moore,I. and Pissarra,J. Dissecting cardosin B trafficking pathways in heterologous systems Planta (2010) 232, 1517-1530. PubMed Europe PubMed DOI
Ibrahim,A.R., Kawamoto,S., Aki,T., Shimada,Y., Rikimaru,S., Onishi,N., Babiker,E.E., Oiso,I., Hashimoto,K., Hayashi,T. and Ono,K. Molecular cloning and immunochemical characterization of a novel major Japanese cedar pollen allergen belonging to the aspartic protease family Int Arch Allergy Immunol (2010) 152, 207-218. PubMed Europe PubMed DOI
Mazorra-Manzano,M.A., Tanaka,T., Dee,D.R. and Yada,R.Y. Structure-function characterization of the recombinant aspartic proteinase A1 from Arabidopsis thaliana Phytochemistry (2010) 71, 515-523. PubMed Europe PubMed DOI
Munoz,F.F., Mendieta,J.R., Pagano,M.R., Paggi,R.A., Daleo,G.R. and Guevara,M.G. The swaposin-like domain of potato aspartic protease (StAsp-PSI) exerts antimicrobial activity on plant and human pathogens Peptides (2010) 31, 777-785. PubMed Europe PubMed DOI
Oliveira,A., Pereira,C., Costa,D.S., Teixeira,J., Fidalgo,F., Pereira,S. and Pissarra,J. Characterization of aspartic proteinases in C. cardunculus L. callus tissue for its prospective transformation Plant Sci (2010) 178, 140-146. DOI
Timotijevic,G.S., Milisavljevic,M.D., Radovic,S.R., Konstantinovic,M.M. and Maksimovic,V.R. Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat J Plant Physiol (2010) 167, 61-68. PubMed Europe PubMed DOI
Timotijevic,G.S., Milisavljevic,M.D., Radovic,S.R., Konstantinovic,M.M. and Maksimovic,V.R. Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench) Arch Biol Sci (2010) 62, 143-152. DOI

2009

Huang,G.J., Huang,S.S., Chen,H., Chang,Y.S., Chang,S.J., Chang,H.Y., Hsieh,P.C., Chang,M.J., Lin,Y.C. and Lin,Y.H. Cloning and expression of aspartic proteinase cDNA from sweet potato storage roots Bot Stud (2009) 50, 149-158.
Sarmento,A.C., Lopes,H., Oliveira,C.S., Vitorino,R., Samyn,B., Sergeant,K., Debyser,G., Van Beeumen,J., Domingues,P., Amado,F., Pires,E., Domingues,M.R. and Barros,M.T. Multiplicity of aspartic proteinases from Cynara cardunculus L Planta (2009) 230, 429-439. PubMed Europe PubMed DOI

2008

Duarte,P., Pissarra,J. and Moore,I. Processing and trafficking of a single isoform of the aspartic proteinase cardosin A on the vacuolar pathway Planta (2008) 227, 1255-1268. PubMed Europe PubMed DOI
Galan,E., Prados,F., Pino,A., Tejada,L. and Fernandez-Salguero,J. Influence of different amounts of vegetable coagulant from cardoon Cynara cardunculus and calf rennet on the proteolysis and sensory characteristics of cheeses made with sheep milk Int Dairy J (2008) 18, 93-98. DOI
Kulkarni,A., Gaikwad,S. and Rao,M. pH induced structural alterations in an aspartic protease from Vigna radiata indicating an alkali induced molten globule state Int J Biol Macromol (2008) 43, 373-376. PubMed Europe PubMed DOI
Mazorra-Manzano,M.A. and Yada,R.Y. Expression and characterization of the recombinant aspartic proteinase A1 from Arabidopsis thaliana Phytochemistry (2008) 69, 2439-2448. PubMed Europe PubMed DOI
Milisavljevic,M.D., Timotijevic,G.S., Radovic,S.R., Konstantinovic,M.M. and Maksimovic,V.R. Two types of aspartic proteinases from buckwheat seed - gene structure and expression analysis J Plant Physiol (2008) 165, 983-990. PubMed Europe PubMed DOI
Pereira,C.S., da Costa,D.S., Pereira,S., Nogueira,F., Albuquerque,P.M., Teixeira,J., Faro,C. and Pissarra,J. Cardosins in postembryonic development of cardoon: towards an elucidation of the biological function of plant aspartic proteinases Protoplasma (2008) 232, 203-213. PubMed Europe PubMed DOI
Raposo,S. and Domingos,A. Purification and characterization milk-clotting aspartic proteinases from Centaurea calcitrapa cell suspension cultures Process Biochem (2008) 43, 139-144. DOI
Sampaio,P.N., Fortes,A.M., Cabral,J.M., Pais,M.S. and Fonseca,L.P. Production and characterization of recombinant cyprosin B in Saccharomyces cerevisiae (W303-1A) strain J Biosci Bioeng (2008) 105, 305-312. PubMed Europe PubMed DOI E
Takahashi,K., Niwa,H., Yokota,N., Kubota,K. and Inoue,H. Widespread tissue expression of nepenthesin-like aspartic protease genes in Arabidopsis thaliana Plant Physiol Biochem (2008) 46, 724-729. PubMed Europe PubMed DOI
Van Der Hoorn,R.A. Plant proteases: from phenotypes to molecular mechanisms Annu Rev Plant Biol (2008) 59, 191-223. PubMed Europe PubMed DOI V

2007

Kulkarni,A. and Rao,M. Biochemical characterization of an aspartic protease from Vigna radiata: kinetic interactions with the classical inhibitor pepstatin implicating a tight binding mechanism Biochim Biophys Acta (2007) 1774, 619-627. PubMed Europe PubMed DOI I
Pagano,M.R., Mendieta,J.R., Munoz,F.F., Daleo,G.R. and Guevara,M.G. Roles of glycosylation on the antifungal activity and apoplast accumulation of StAPs (Solanum tuberosum aspartic proteases) Int J Biol Macromol (2007) 41, 512-520. PubMed Europe PubMed DOI
Pimentel,C., Van Der Straeten,D., Pires,E., Faro,C. and Rodrigues-Pousada,C. Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L FEBS J (2007) 274, 2523-2539. PubMed Europe PubMed DOI
Tamura,T., Terauchi,K., Kiyosaki,T., Asakura,T., Funaki,J., Matsumoto,I., Misaka,T. and Abe,K. Differential expression of wheat aspartic proteinases, WAP1 and WAP2, in germinating and maturing seeds J Plant Physiol (2007) 164, 470-477. PubMed Europe PubMed DOI

2006

Barros,R.M. and Malcata,F.X. Molecular characterization of peptides released from beta-lactoglobulin and alpha-lactalbumin via cardosins A and B J Dairy Sci (2006) 89, 483-494. PubMed Europe PubMed DOI P
Terauchi,K., Asakura,T., Ueda,H., Tamura,T., Tamura,K., Matsumoto,I., Misaka,T., Hara-Nishimura,I. and Abe,K. Plant-specific insertions in the soybean aspartic proteinases, soyAP1 and soyAP2, perform different functions of vacuolar targeting J Plant Physiol (2006) 163, 856-862. PubMed Europe PubMed DOI

2005

[YEAR:27-1-2005]Castanheira,P., Samyn,B., Sergeant,K., Clemente,J.C., Dunn,B.M., Pires,E., Van Beeumen,J. and Faro,C. Activation, proteolytic processing, and peptide specificity of recombinant cardosin A J Biol Chem (27-1-2005) 280, 13047-13054. PubMed Europe PubMed DOI
[YEAR:27-9-2005]Guevara,M.G., Almeida,C., Mendieta,J.R., Faro,C.J., Verissimo,P., Pires,E.V. and Daleo,G.R. Molecular cloning of a potato leaf cDNA encoding an aspartic protease (StAsp) and its expression after P. infestans infection Plant Physiol Biochem (27-9-2005) 43, 882-889. PubMed Europe PubMed DOI
Sidrach,L., Garcia-Canovas,F., Tudela,J. and Rodriguez-Lopez,J.N. Purification of cynarases from artichoke (Cynara scolymus L.): enzymatic properties of cynarase A Phytochemistry (2005) 66, 41-49. PubMed Europe PubMed DOI

2004

Barros,R.M. and Malcata,F.X. A kinetic model for hydrolysis of whey proteins by cardosin A extracted from Cynara cardunculus Food Chem (2004) 88, 351-359.
de Carvalho,M.H., Pham-Thi,A.T., Gareil,M., d'Arcy-Lameta,A. and Fodil,Y.Z. Isolation and characterization of an aspartic proteinase gene from cowpea (Vigna unguiculata L. Walp.) J Plant Physiol (2004) 161, 971-976. PubMed Europe PubMed DOI
Kervinen,J., Wlodawer,A. and Zdanov,A. Phytepsin [ISSN:0-12-079610-4] (2004) 2, 77-84. V
[YEAR:29-12-2004]Llorente,B.E., Brutti,C.B. and Caffini,N.O. Purification and characterization of a milk-clotting aspartic proteinase from globe artichoke (Cynara scolymus L.) J Agric Food Chem (29-12-2004) 52, 8182-8189. PubMed Europe PubMed DOI
Schaaf,A., Reski,R. and Decker,E.L. A novel aspartic proteinase is targeted to the secretory pathway and to the vacuole in the moss Physcomitrella patens Eur J Cell Biol (2004) 83, 145-152. PubMed Europe PubMed DOI
Simoes,I. and Faro,C. Structure and function of plant aspartic proteinases Eur J Biochem (2004) 271, 2067-2075. PubMed Europe PubMed DOI
Terauchi,K., Asakura,T., Nishizawa,N.K., Matsumoto,I. and Abe,K. Characterization of the genes for two soybean aspartic proteinases and analysis of their different tissue-dependent expression Planta (2004) 218, 947-957. PubMed Europe PubMed DOI

2003

[YEAR:3-12-2003]Fernandez-Salguero,J., Prados,F., Calixto,F., Vioque,M., Sampaio,P. and Tejada,L. Use of recombinant cyprosin in the manufacture of ewe's milk cheese J Agric Food Chem (3-12-2003) 51, 7426-7430. PubMed Europe PubMed DOI E
[YEAR:26-3-2003]Timotijevic,G.S., Radovic,S.R. and Maksimovic,V.R. Characterization of an aspartic proteinase activity in buckwheat (Fagopyrum esculentum moench) seeds J Agric Food Chem (26-3-2003) 51, 2100-2104. PubMed Europe PubMed DOI

2002

An,C.I., Fukusaki,E. and Kobayashi,A. Aspartic proteinases are expressed in pitchers of the carnivorous plant Nepenthes alata Blanco Planta (2002) 214, 661-667. PubMed Europe PubMed DOI
Chen,X., Pfeil,J.E. and Gal,S. The three typical aspartic proteinase genes of Arabidopsis thaliana are differentially expressed Eur J Biochem (2002) 269, 4675-4684. PubMed Europe PubMed DOI

2001

[YEAR:5-11-2001]Barros,R.M., Ferreira,C.A., Silva,S.V. and Malcata,F.X. Quantitative studies on the enzymatic hydrolysis of milk proteins brought about by cardosins precipitated by ammonium sulfate Enzyme Microb Technol (5-11-2001) 29, 541-547.
[YEAR:16-3-2001]Cruz de Carvalho,M.H., Arcy-Lameta,A., Roy-Macauley,H., Gareil,M., El Maarouf,H., Pham-Thi,A.T. and Zuily-Fodil,Y. Aspartic protease in leaves of common bean (Phaseolus vulgaris L.) and cowpea (Vigna unguiculata L. Walp): enzymatic activity, gene expression and relation to drought susceptibility FEBS Lett (16-3-2001) 492, 242-246. PubMed Europe PubMed DOI
Guevara,M.G., Daleo,G.R. and Oliva,C.R. Purification and characterization of an aspartic protease from potato leaves Physiol Plant (2001) 112, 321-326. PubMed Europe PubMed
Park,H., Kusakabe,I., Sakakibara,Y. and Kobayashi,H. Autoproteolytic processing of aspartic proteinase from sunflower seeds Biosci Biotechnol Biochem (2001) 65, 702-705. PubMed Europe PubMed DOI
Tormakangas,K., Hadlington,J.L., Pimpl,P., Hillmer,S., Brandizzi,F., Teeri,T.H. and Denecke,J. A vacuolar sorting domain may also influence the way in which proteins leave the endoplasmic reticulum Plant Cell (2001) 13, 2021-2032. PubMed Europe PubMed DOI PMC EPMC
Vieira,M., Pissarr,J., Verissimo,P., Castanheira,P., Costa,Y., Pires,E. and Faro,C. Molecular cloning and characterization of cDNA encoding cardosin B, an aspartic proteinase accumulating extracellularly in the transmitting tissue of Cynara cardunculus L Plant Mol Biol (2001) 45, 529-539. PubMed Europe PubMed DOI

2000

Asakura,T., Matsumoto,I., Funaki,J., Arai,S. and Abe,K. The plant aspartic proteinase-specific polypeptide insert is not directly related to the activity of oryzasin 1 Eur J Biochem (2000) 267, 5115-5122. PubMed Europe PubMed DOI
[YEAR:8-12-2000]Egas,C., Lavoura,N., Resende,R., Brito,R.M., Pires,E., de Lima,M.C. and Faro,C. The saposin-like domain of the plant aspartic proteinase precursor is a potent inducer of vesicle leakage J Biol Chem (8-12-2000) 275, 38190-38196. PubMed Europe PubMed DOI
Lindholm,P., Kuittinen,T., Sorri,O., Guo,D., Merits,A., Tormakangas,K. and Runeberg-Roos,P. Glycosylation of phytepsin and expression of dad1, dad2 and ost1 during onset of cell death in germinating barley scutella Mech Dev (2000) 93, 169-173. PubMed Europe PubMed DOI
Park,H., Yamanaka,N., Mikkonen,A., Kusakabe,I. and Kobayashi,H. Purification and characterization of aspartic proteinase from sunflower seeds Biosci Biotechnol Biochem (2000) 64, 931-939. PubMed Europe PubMed
Payie,K.G., Weadge,J.T., Tanaka,T. and Yada,R.Y. Purification, N-terminal sequencing and partial characterization of a novel aspartic proteinase from the leaves of Medicago sativa L. (alfalfa) Biotechnol Lett (2000) 22, 1515-1520.
[YEAR:1-11-2000]Silva,S.V. and Malcata,F.X. Comparative catalytic activity of two plant proteinases upon caprine caseins in solution Food Chem (1-11-2000) 71, 207-214. DOI P
Silva,S.V. and Malcata,F.X. Action of cardosin A from Cynara humilis on ovine and caprine caseinates J Dairy Res (2000) 67, 449-454. PubMed Europe PubMed DOI P
Vioque,M., Gomez,R., Sanchez,E., Mata,C., Tejada,L. and Fernandez-Salguero,J. Chemical and microbiological characteristics of ewes' milk cheese manufactured with extracts from flowers of Cynara cardunculus and Cynara humilis as coagulants J Agric Food Chem (2000) 48, 451-456. PubMed Europe PubMed DOI

1999

Amidon,W.J., Pfeil,J.E. and Gal,S. Modification of luciferase to be a substrate for plant aspartic proteinase Biochem J (1999) 343, 425-433. PubMed Europe PubMed DOI PMC EPMC A
Bernstein,N.K. and James,M.N. Novel ways to prevent proteolysis - prophytepsin and proplasmepsin II Curr Opin Struct Biol (1999) 9, 684-689. PubMed Europe PubMed S
Brijs,K., Bleukx,W. and Delcour,J.A. Proteolytic activities in dormant rye (Secale cereale L.) grain J Agric Food Chem (1999) 47, 3572-3578. PubMed Europe PubMed DOI
[YEAR:1-10-1999]Faro,C., Ramalho-Santos,M., Vieira,M., Mendes,A., Simoes,I., Andrade,R., Verissimo,P., Lin,X., Tang,J. and Pires,E. Cloning and characterization of cDNA encoding cardosin A, an RGD-containing plant aspartic proteinase J Biol Chem (1-10-1999) 274, 28724-28729. PubMed Europe PubMed DOI
[YEAR:24-9-1999]Frazao,C., Bento,I., Costa,J., Soares,C.M., Verissimo,P., Faro,C., Pires,E., Cooper,J. and Carrondo,M.A. Crystal structure of cardosin A, a glycosylated and Arg-Gly-Asp-containing aspartic proteinase from the flowers of Cynara cardunculus L J Biol Chem (24-9-1999) 274, 27694-27701. PubMed Europe PubMed DOI S
Guevara,M.G., Oliva,C.R., Machinandiarena,M. and Daleo,G.R. Purification and properties of an aspartic protease from potato tuber that is inhibited by a basic chitinase Physiol Plant (1999) 106, 164-169.
[YEAR:15-7-1999]Kervinen,J., Tobin,G.J., Costa,J., Waugh,D.S., Wlodawer,A. and Zdanov,A. Crystal structure of plant aspartic proteinase prophytepsin: inactivation and vacuolar targeting EMBO J (15-7-1999) 18, 3947-3955. PubMed Europe PubMed DOI PMC EPMC S
Mutlu,A., Chen,X., Reddy,S.M. and Gal,S. The aspartic proteinase is expressed in Arabidopsis thaliana seeds and localized in the protein bodies Seed Sci Res. (1999) 9, 75-84.
Mutlu,A. and Gal,S. Plant aspartic proteinases: enzymes on the way to a function Physiol Plant (1999) 105, 569-576.
[YEAR:11-6-1999]White,P.C., Cordeiro,M.C., Arnold,D., Brodelius,P.E. and Kay,J. Processing, activity, and inhibition of recombinant cyprosin, an aspartic proteinase from cardoon (Cynara cardunculus) J Biol Chem (11-6-1999) 274, 16685-16693. PubMed Europe PubMed DOI E
Zhang,N. and Jones,B.L. Polymorphism of aspartic proteinases in resting and germinating barley seeds Cereal Chem (1999) 76, 134-138.

1998

[YEAR:8-9-1998]Bleukx,W., Brijs,K., Torrekens,S., Van Leuven,F. and Delcour,J.A. Specificity of a wheat gluten aspartic proteinase Biochim Biophys Acta (8-9-1998) 1387, 317-324. PubMed Europe PubMed DOI
Brodelius,P.E., Cordeiro,M., Mercke,P., Domingos,A., Clemente,A. and Pais,M.S. Molecular cloning of aspartic proteinases from flowers of Cynara cardunculus subsp. flavescens cv. cardoon and Centaurea calcitrapa Adv Exp Med Biol (1998) 436, 435-439. PubMed Europe PubMed
Cordeiro,M., Lowther,T., Dunn,B.M., Guruprasad,K., Blundell,T., Pais,M.S. and Brodelius,P.E. Substrate specificity and molecular modelling of aspartic proteinases (cyprosins) from flowers of Cynara cardunculus subsp. flavescens cv. cardoon Adv Exp Med Biol (1998) 436, 473-479. PubMed Europe PubMed
Domingos,A., Xue,Z.T., Guruprasad,K., Clemente,A., Blundell,T., Pais,M.S. and Brodelius,P.E. An aspartic proteinase from flowers of Centaurea calcitrapa. Purification, characterization, molecular cloning, and modelling of its three-dimensional structure Adv Exp Med Biol (1998) 436, 465-472. PubMed Europe PubMed
Faro,C., Ramalho-Santos,M., Verissimo,P., Pissarra,J., Frazao,C., Costa,J., Lin,X.L., Tang,J. and Pires,E. Structural and functional aspects of cardosins Adv Exp Med Biol (1998) 436, 423-433. PubMed Europe PubMed
[YEAR:20-11-1998]Glathe,S., Kervinen,J., Nimtz,M., Li,G.H., Tobin,G.J., Copeland,T.D., Ashford,D.A., Wlodawer,A. and Costa,J. Transport and activation of the vacuolar aspartic proteinase phytepsin in barley (Hordeum vulgare L.) J Biol Chem (20-11-1998) 273, 31230-31236. PubMed Europe PubMed DOI
Mutlu,A., Pfeil,J.E. and Gal,S. A probarley lectin processing enzyme purified from Arabidopsis thaliana seeds Phytochemistry (1998) 47, 1453-1459. PubMed Europe PubMed DOI
[YEAR:1-7-1998]Ramalho-Santos,M., Verissimo,P., Cortes,L., Samyn,B., Van Beeumen,J., Pires,E. and Faro,C. Identification and proteolytic processing of procardosin A Eur J Biochem (1-7-1998) 255, 133-138. PubMed Europe PubMed DOI
Runeberg-Roos,P. and Saarma,M. Phytepsin, a barley vacuolar aspartic proteinase, is highly expressed during autolysis of developing tracheary elements and sieve cells Plant J (1998) 15, 139-145. PubMed Europe PubMed DOI

1997

Asakura,T., Watanabe,H., Abe,K. and Arai,S. Oryzasin as an aspartic proteinase occurring in rice seeds: purification, characterization, and application to milk clotting J Agric Food Chem (1997) 45, 1070-1075.
D'Hondt,K., Stack,S., Gutteridge,S., Vandekerckhove,J., Krebbers,E. and Gal,S. Aspartic proteinase genes in the Brassicaceae Arabidopsis thaliana and Brassica napus Plant Mol Biol (1997) 33, 187-192. PubMed Europe PubMed DOI

1996

Ramalho-Santos,M., Verissimo,P., Faro,C. and Pires,E. Action on bovine alphas1-casein of cardosins A and B, aspartic proteinases from the flowers of the cardoon Cynara cardunculus L Biochim Biophys Acta (1996) 1297, 83-89. PubMed Europe PubMed DOI
Schaller,A. and Ryan,C.A. Molecular cloning of a tomato leaf cDNA encoding an aspartic protease, a systemic wound response protein Plant Mol Biol (1996) 31, 1073-1077. PubMed Europe PubMed
Verissimo,P., Faro,C., Moir,A.J., Lin,Y., Tang,J. and Pires,E. Purification, characterization and partial amino acid sequencing of two new aspartic proteinases from fresh flowers of Cynara cardunculus L Eur J Biochem (1996) 235, 762-768. PubMed Europe PubMed

1995

Asakura,T., Abe,K. and Arai,S. Evidence for the occurrence of multiple aspartic proteinases in rice seeds Biosci Biotechnol Biochem (1995) 59, 1793-1794. PubMed Europe PubMed DOI
Asakura,T., Watanabe,H., Abe,K. and Arai,S. Rice aspartic proteinase, oryzasin, expressed during seed ripening and germination, has a gene organization distinct from those of animal and microbial aspartic proteinases Eur J Biochem (1995) 232, 77-83. PubMed Europe PubMed DOI
Cordeiro,M.C., Xue,Z.T., Pietrzak,M., Pais,M.S. and Brodelius,P.E. Plant aspartic proteinases from Cynara cardunculus spp. flavescens cv. cardoon; nucleotide sequence of a cDNA encoding cyprosin and its organ-specific expression Adv Exp Med Biol (1995) 362, 367-372. PubMed Europe PubMed
Marttila,S., Jones,B.L. and Mikkonen,A. Differential localization of two acid proteinases in germinating barley (Hordeum vulgare) seed Physiol Plant (1995) 93, 317-327.

1994

Cordeiro,M.C., Xue,Z.T., Pietrzak,M., Pais,M.S. and Brodelius,P.E. Isolation and characterization of a cDNA from flowers of Cynara cardunculus encoding cyprosin (an aspartic proteinase) and its use to study the organ-specific expression of cyprosin Plant Mol Biol (1994) 24, 733-741. PubMed Europe PubMed
Cordeiro,M.C., Pais,M.S. and Brodelius,P.E. Tissue-specific expression of multiple forms of cyprosin (aspartic proteinase) in flowers of Cynara cardunculus Physiol Plant (1994) 92, 645-653.
Galleschi,L. and Felicioli,F. Purification, characterization and activation by anions of an aspartic proteinase isolated from bran of soft wheat Plant Sci (1994) 98, 15-24.
Runeberg-Roos,P., Kervinen,J., Kovaleva,V., Raikhel,N.V. and Gal,S. The aspartic proteinase of barley is a vacuolar enzyme that processes probarley lectin in vitro Plant Physiol (1994) 105, 321-329. PubMed Europe PubMed PMC EPMC

1993

Kervinen,J., Sarkkinen,P., Kalkkinen,N., Mikola,L. and Saarma,M. Hydrolytic specificity of the barley grain aspartic proteinase Phytochemistry (1993) 32, 799-803. PubMed Europe PubMed

1992

Faro,C.J., Moir,A.J.G. and Pires,E.V. Specificity of a milk clotting enzyme extracted from the thistle Cynara cardunculus L.: action on oxidized insulin and kappa-casein Biotechnol Lett (1992) 14, 841-846. DOI
Sarkkinen,P., Kalkkinen,N., Tilgmann,C., Siuro,J., Kervinen,J. and Mikola,L. Aspartic proteinase from barley grains is related to mammalian lysosomal cathepsin D Planta (1992) 186, 317-323. PubMed Europe PubMed DOI

1991

Runeberg-Roos,P., Tormakangas,K. and Ostman,A. Primary structure of a barley-grain aspartic proteinase. A plant aspartic proteinase resembling mammalian cathepsin D Eur J Biochem (1991) 202, 1021-1027. PubMed Europe PubMed

1980

Dol,E., Shibata,D., Matoba,T. and Yonezawa,D. Characterization of pepstatin-sensitive acid protease in resting rice seeds Agric Biol Chem (1980) 44, 741-747. DOI

1978

Frith,G.J.T., Peoples,M.B. and Dalling,M.J. Proteolytic enzymes in green wheat-leaves III. Inactivation of acid proteinase II by diazoacetyl-DL-norleucine methyl ester and 1,2-epoxy-3-(p-nitrophenoxy)-propane Plant Cell Physiol (1978) 19, 819-824. I

1971

Shinano,S. and Fukushima,K. Studies on lotus seed protease. 3. Some physicochemical and enzymic properties Agric Biol Chem (1971) 35, 1488-1494.

1970

Garg,G.K. and Virupaksha,T.K. Acid protease from germinated sorghum. 2. Substrate specificity with synthetic peptides and ribonuclease A Eur J Biochem (1970) 17, 13-18. PubMed Europe PubMed