$VAR1 = undef;

Summary for peptidase C01.002: chymopapain

Names
MEROPS Name	chymopapain
Other names	chymopapain A, chymopapain B

Domain architecture

MEROPS Classification
Classification	Clan CA >> Subclan (none) >> Family C1 >> Subfamily A >> C01.002
Holotype	chymopapain (Carica papaya), Uniprot accession P14080 (peptidase unit: 135-350), MERNUM MER0000706
History	Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.

Activity
Catalytic type	Cysteine
Peplist	Included in the Peplist with identifier PL00047
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.6
Enzymology	BRENDA database
Biotechnology	Contact-lens cleaning fluid. Stem cell isolation.
Pharmaceutical relevance	Treatment of herniated intervertebral disk (patent).
Other databases	WIKIPEDIA	http://en.wikipedia.org/wiki/Chymopapain
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	-/-/vf/rg/-/-/- (based on 12 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	1	2	0	0	3	0	0	1
Pro	0	2	0	0	0	1	1	0
Ala	2	0	0	0	2	0	0	1
Val	0	0	5	0	0	0	0	0
Leu	0	2	1	0	0	1	1	2
Ile	0	0	0	0	0	0	0	0
Met	0	0	0	0	0	0	0	0
Phe	0	2	3	1	0	1	1	0
Tyr	1	0	0	2	1	0	1	1
Trp	0	0	0	0	0	0	0	0
Ser	0	0	0	0	0	1	0	0
Thr	0	0	0	0	1	1	0	0
Cys	0	0	0	0	0	0	0	0
Asn	0	0	0	1	0	0	0	0
Gln	1	0	0	0	1	0	0	0
Asp	0	0	0	0	0	0	0	0
Glu	0	0	1	1	0	1	0	0
Lys	0	0	0	2	0	0	1	1
Arg	1	0	0	3	0	0	1	0
His	1	1	0	0	0	1	1	0

Specificity from combinatorial peptides

Organism	comment	P4	P3	P2	P1	P1'	P2'	P3'	P4'	optimal substrate	fluorophore or acceptor-donor pair	Reference
Carica papaya	wild type	-	E/I/L	M/F/P	R/C	-	-	-	-	xEMRxxxx	ACC-Dabcyl	Sun et al., 2007
Carica papaya	wild type	A	F/P/V	F/broad	K/V/S/F	-	-	-	-	AFFKxxxx	FAM-TAMRA	Diaz-Mochon et al., 2006