$VAR1 = undef;

Summary for peptidase S01.136: granzyme B, rodent-type

Names
MEROPS Name	granzyme B, rodent-type
Other names	asp-ase, CCP II, CTLA1, CGL-1, cytotoxic cell protease 2, fragmentin, GzmBP (Mus musculus), GzmBW (Mus musculus)

Domain architecture

MEROPS Classification
Classification	Clan PA >> Subclan PA(S) >> Family S1 >> Subfamily A >> S01.136
Holotype	granzyme B, rodent-type (Mus musculus), Uniprot accession P04187 (peptidase unit: 21-247), MERNUM MER0000160
History	Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.

Activity
Catalytic type	Serine
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.79
Enzymology	BRENDA database
Proteolytic events	CutDB database (23 cleavages)
Physiology	Role in triggering of apoptosis by cytotoxic cell granules, activating caspases.
Other databases	TREEFAM	http://www.treefam.org/family/TF333630
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	v/-/-/D-/-/-/- (based on 493 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	5	61	64	0	34	76	18	26
Pro	41	1	77	1	0	3	14	17
Ala	36	40	51	5	72	52	26	33
Val	102	26	60	0	32	15	55	43
Leu	78	47	40	5	62	94	35	46
Ile	74	8	5	0	21	7	38	31
Met	19	10	12	2	7	6	6	10
Phe	21	9	17	0	14	17	11	20
Tyr	7	7	5	2	6	17	27	4
Trp	8	1	1	0	1	0	0	0
Ser	12	35	33	4	75	48	36	33
Thr	12	24	19	2	47	22	37	28
Cys	6	9	0	0	1	1	2	0
Asn	4	20	21	10	14	8	11	8
Gln	11	14	26	0	19	16	12	23
Asp	5	55	10	408	16	43	33	67
Glu	32	74	36	51	34	48	68	76
Lys	8	12	10	1	31	12	58	21
Arg	0	11	0	1	0	1	0	1
His	5	24	2	1	1	1	0	0

Specificity from combinatorial peptides

Organism	comment	P4	P3	P2	P1	P1'	P2'	P3'	P4'	optimal substrate	fluorophore or acceptor-donor pair	Reference
Rattus norvegicus	wild type	I/V	E	P/n/A	D	-	-	-	-	IEPD	AMC	Ruggles et al., 2004
Rattus norvegicus	recombinant	nd	nd	nd	D	-	-	-	-	D	ACC	Harris et al., 2000

Mouse genetics
Gene symbol	Position	Megabases	Ensembl	Entrez gene	MGI
Gzmb	14:C1		ENSMUSG00000015437	14939	MGI:109267