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VelcroVax tandem HBcAg with SUMO-Affimer inserted at MIR (T=4 VLP)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7zq8
Q-score: 0.612
Kingston NJ, Grehan K, Snowden JS, Hassall M, Alzahrani J, Paesen GC, Sherry L, Hayward C, Roe A, Stephen S, Tomlinson D, Zeltina A, Doores KJ, Ranson NA, Stacey M, Page M, Rose NJ, Bowden TA, Rowlands DJ, Stonehouse NJ
mSphere (2023) 8 pp. e0056822-e0056822 [ Pubmed: 36719225 DOI: doi:10.1128/msphere.00568-22 ]
VelcroVax tandem HBcAg with SUMO-Affimer inserted at MIR (T=3* VLP)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7zqa
Q-score: 0.513
Kingston NJ, Grehan K, Snowden JS, Hassall M, Alzahrani J, Paesen GC, Sherry L, Hayward C, Roe A, Stephen S, Tomlinson D, Zeltina A, Doores KJ, Ranson NA, Stacey M, Page M, Rose NJ, Bowden TA, Rowlands DJ, Stonehouse NJ
mSphere (2023) 8 pp. e0056822-e0056822 [ Pubmed: 36719225 DOI: doi:10.1128/msphere.00568-22 ]
Dicks MDJ, Rose LM, Russell RA, Bowman LAH, Graham C, Jimenez-Guardeno JM, Doores KJ, Malim MH, Draper SJ, Howarth M, Biswas S
Mol Ther (2022) 30 pp. 3639-3657 [ DOI: doi:10.1016/j.ymthe.2022.08.002 Pubmed: 35949171 ]
Dicks MDJ, Rose LM, Russell RA, Bowman LAH, Graham C, Jimenez-Guardeno JM, Doores KJ, Malim MH, Draper SJ, Howarth M, Biswas S
Mol Ther (2022) 30 pp. 3639-3657 [ DOI: doi:10.1016/j.ymthe.2022.08.002 Pubmed: 35949171 ]
CryoEM structure of SARS-CoV-2 spike monomer in complex with neutralising antibody P008_60
Resolution: 4.31 Å
EM Method: Single-particle
Fitted PDBs: 7zbu
Q-score: 0.209
Seow J, Khan H, Rosa A, Calvaresi V, Graham C, Pickering S, Pye VE, Cronin NB, Huettner I, Malim MH, Politis A, Cherepanov P, Doores KJ
Cell Rep (2022) 40 pp. 111276-111276 [ Pubmed: 35981534 DOI: doi:10.1016/j.celrep.2022.111276 ]
- P008_60 antibody, light chain (23 kDa, Protein from Homo sapiens)
- P008_60 antibody, heavy chain (25 kDa, Protein from Homo sapiens)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- 3-[5-[(4-ethyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-2-[[5-[(3-ethyl-4-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1h-pyrrol-2-yl]methyl]-4-methyl-1h-pyrrol-3-yl]propanoic acid (588 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Monomeric spike glycoprotein ectodomain from sars-cov-2 in complex with a neutralising antibody p008_60 (125 kDa, Complex from Homo sapiens)
Hantaan virus-like particle glycoprotein lattice.
Rissanen I, Krumm SA, Stass R, Whitaker A, Voss JE, Bruce EA, Rothenberger S, Kunz S, Burton DR, Huiskonen JT, Botten JW, Bowden TA, Doores KJ
Mbio (2021) 12 pp. e0253120-e0253120 [ Pubmed: 34225492 DOI: doi:10.1128/mBio.02531-20 ]
Hantaan virus-like particle glycoprotein spike in complex with Fab fragment HTN-Gn1.
Resolution: 19.0 Å
EM Method: Subtomogram averaging
Fitted PDBs: 7nrh
Q-score: 0.05
Rissanen I, Krumm SA, Stass R, Whitaker A, Voss JE, Bruce EA, Rothenberger S, Kunz S, Burton DR, Huiskonen JT, Botten JW, Bowden TA, Doores KJ
Mbio (2021) 12 pp. e0253120-e0253120 [ Pubmed: 34225492 DOI: doi:10.1128/mBio.02531-20 ]
- Fab fragment htn-gn1 light chain (22 kDa, Protein from Oryctolagus cuniculus)
- Hantaan orthohantavirus (Virus from Hantaan orthohantavirus)
- Envelope polyprotein (40 kDa, Protein from Hantaan orthohantavirus)
- Fab fragment htn-gn1 heavy chain (24 kDa, Protein from Oryctolagus cuniculus)
Trimeric SARS-CoV-2 spike ectodomain in complex with biliverdin (closed conformation)
Resolution: 3.36 Å
EM Method: Single-particle
Fitted PDBs: 7nt9
Q-score: 0.458
Rosa A, Pye VE, Graham C, Muir L, Seow J, Ng KW, Cook NJ, Rees-Spear C, Parker E, Dos Santos MS, Rosadas C, Susana A, Rhys H, Nans A, Masino L, Roustan C, Christodoulou E, Ulferts R, Wrobel AG, Short CE, Fertleman M, Sanders RW, Heaney J, Spyer M, Kjaer S, Riddell A, Malim MH, Beale R, MacRae JI, Taylor GP, Nastouli E, van Gils MJ, Rosenthal PB, Pizzato M, McClure MO, Tedder RS, Kassiotis G, McCoy LE, Doores KJ, Cherepanov P
Sci Adv (2021) 7 [ Pubmed: 33888467 DOI: doi:10.1126/sciadv.abg7607 ]
- Ectodomain of spike glycoprotein from sars-cov-2 expressed in hek cells and purified. data were collected in the presence of excess biliverdin. (426 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Biliverdine ix alpha (582 Da, Ligand)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Water (18 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Trimeric SARS-CoV-2 spike ectodomain in complex with biliverdin (one RBD erect)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7nta
Q-score: 0.436
Rosa A, Pye VE, Graham C, Muir L, Seow J, Ng KW, Cook NJ, Rees-Spear C, Parker E, Dos Santos MS, Rosadas C, Susana A, Rhys H, Nans A, Masino L, Roustan C, Christodoulou E, Ulferts R, Wrobel AG, Short CE, Fertleman M, Sanders RW, Heaney J, Spyer M, Kjaer S, Riddell A, Malim MH, Beale R, MacRae JI, Taylor GP, Nastouli E, van Gils MJ, Rosenthal PB, Pizzato M, McClure MO, Tedder RS, Kassiotis G, McCoy LE, Doores KJ, Cherepanov P
Sci Adv (2021) 7 [ Pubmed: 33888467 DOI: doi:10.1126/sciadv.abg7607 ]
- Biliverdine ix alpha (582 Da, Ligand)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Water (18 Da, Ligand)
- Ectodomain of s1 protein from sars-cov-2 expressed in hek cells and purified. sample in the presence of excess biliverdin. (426 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Trimeric SARS-CoV-2 spike ectodomain bound to P008_056 Fab
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7ntc
Q-score: 0.391
Rosa A, Pye VE, Graham C, Muir L, Seow J, Ng KW, Cook NJ, Rees-Spear C, Parker E, Dos Santos MS, Rosadas C, Susana A, Rhys H, Nans A, Masino L, Roustan C, Christodoulou E, Ulferts R, Wrobel AG, Short CE, Fertleman M, Sanders RW, Heaney J, Spyer M, Kjaer S, Riddell A, Malim MH, Beale R, MacRae JI, Taylor GP, Nastouli E, van Gils MJ, Rosenthal PB, Pizzato M, McClure MO, Tedder RS, Kassiotis G, McCoy LE, Doores KJ, Cherepanov P
Sci Adv (2021) 7 [ Pubmed: 33888467 DOI: doi:10.1126/sciadv.abg7607 ]
- P008_056 fab heavy chain (27 kDa, Protein from Homo sapiens)
- P008_056 fab light chain (25 kDa, Protein from Homo sapiens)
- P008_056 fab (Complex from Homo sapiens)
- Biliverdine ix alpha (582 Da, Ligand)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Stabilised sars-cov-2 spike ectodomain trimer bound to one p008_056 fab (478 kDa, Complex)
- Stabilised sars-cov-2 spike ectodomain trimer (Complex from Severe acute respiratory syndrome coronavirus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)