EMDB EMD-8827

Single particle reconstruction
3.64Å resolution

Cas1-Cas2-IHF-DNA holo-complex

Map released:
Last modified:
Overview of EMD-8827
Source organisms:
Fitted atomic model: 5wfe
Primary publication:
Structures of the CRISPR genome integration complex.
Wright AV, Liu JJ, Knott GJ, Doxzen KW, Nogales E, Doudna JA
Science 357 1113-1118 (2017)
PMID: 28729350

Function and Biology Details

Sample name: Cas1-Cas2-IHF-DNA holo complex
Nucleic acids: DNA (28-MER), DNA (45-MER), DNA (76-MER), DNA (61-MER)
Proteins: Cas1-Cas2-IHF-DNA holo complex, CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, Integration host factor subunit alpha, Integration host factor subunit beta

Experimental Information Details

Resolution: 3.64Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)