5wfe

Electron Microscopy
3.64Å resolution

Cas1-Cas2-IHF-DNA holo-complex

Released:
Related structures: EMD-8827

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
4 distinct polypeptide molecules
4 distinct DNA molecules
Macromolecules (8 distinct):
CRISPR-associated endonuclease Cas1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 305 amino acids
Theoretical weight: 33.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46896 (Residues: 1-305; Coverage: 100%)
Gene names: JW2725, b2755, cas1, ygbT
Sequence domains: CRISPR associated protein Cas1
CRISPR-associated endoribonuclease Cas2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 103 amino acids
Theoretical weight: 11.55 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P45956 (Residues: 1-94; Coverage: 100%)
Gene names: JW5438, b2754, cas2, ygbF
Sequence domains: CRISPR-associated protein (Cas_Cas2CT1978)
Integration host factor subunit alpha Chain: K
Molecule details ›
Chain: K
Length: 99 amino acids
Theoretical weight: 11.37 KDa
Source organism: Escherichia coli S88
Expression system: Escherichia coli
UniProt:
  • Canonical: B7MAS3 (Residues: 1-99; Coverage: 100%)
Gene names: ECS88_1763, himA, ihfA
Sequence domains: Bacterial DNA-binding protein
Integration host factor subunit beta Chain: L
Molecule details ›
Chain: L
Length: 94 amino acids
Theoretical weight: 10.67 KDa
Source organism: Escherichia coli S88
Expression system: Escherichia coli
UniProt:
  • Canonical: B7MHM1 (Residues: 1-94; Coverage: 100%)
Gene names: ECS88_0940, himD, ihfB
Sequence domains: Bacterial DNA-binding protein
DNA (28-MER) Chain: G
Molecule details ›
Chain: G
Length: 28 nucleotides
Theoretical weight: 8.68 KDa
DNA (45-MER) Chain: H
Molecule details ›
Chain: H
Length: 61 nucleotides
Theoretical weight: 18.75 KDa
DNA (76-MER) Chain: I
Molecule details ›
Chain: I
Length: 95 nucleotides
Theoretical weight: 29.1 KDa
DNA (61-MER) Chain: J
Molecule details ›
Chain: J
Length: 62 nucleotides
Theoretical weight: 19.29 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.64Å
Relevant EMDB volumes: EMD-8827
Expression system: Escherichia coli