EMDB EMD-8481

Single particle reconstruction
3.9Å resolution

Structure of tetrameric HIV-1 Strand Transfer Complex Intasome

Map released:
Last modified:
Overview of EMD-8481
Source organisms:
Fitted atomic model: 5u1c
Related EM entry by publication: EMD-8483
Primary publication:
Cryo-EM structures and atomic model of the HIV-1 strand transfer complex intasome.
Passos DO, Li M, Yang R, Rebensburg SV, Ghirlando R, Jeon Y, Shkriabai N, Kvaratskhelia M, Craigie R, Lyumkis D
Science 355 89-92 (2017)
PMID: 28059769

Function and Biology Details

Sample name: complex formed by a tetrameric assembly of Sso7d-fusion HIV-1 Integrase with the product of DNA strand transfer
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: DNA (11-MER), DNA (23-MER), DNA (37-MER)
Proteins: complex formed by a tetrameric assembly of Sso7d-fusion HIV-1 Integrase with the product of DNA strand transfer, HIV-1 Integrase, Sso7d chimera

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: FREALIGN
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)