5u1c

Electron Microscopy
3.9Å resolution

Structure of tetrameric HIV-1 Strand Transfer Complex Intasome

Released:

Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Integrase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 383 amino acids
Theoretical weight: 42.32 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12497 (Residues: 1132-1136, 1148-1435; Coverage: 20%)
Gene name: gag-pol
Sequence domains:
DNA (11-MER) Chains: G, H
Molecule details ›
Chains: G, H
Length: 11 nucleotides
Theoretical weight: 3.35 KDa
DNA (23-MER) Chains: E, I
Molecule details ›
Chains: E, I
Length: 23 nucleotides
Theoretical weight: 7.02 KDa
DNA (37-MER) Chains: F, J
Molecule details ›
Chains: F, J
Length: 37 nucleotides
Theoretical weight: 11.41 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-8481
Expression system: Escherichia coli