EMDB EMD-7563

Single particle reconstruction
6.8Å resolution

Structure of the cargo bound AP-1:Arf1:tetherin-Nef stable closed trimer

Map released:
Last modified:
Overview of EMD-7563
Source organisms:
Fitted atomic model: 6cri
Related EM entries by publication: EMD-7453, EMD-7454, EMD-7455, EMD-7456, EMD-7457, EMD-7458
Primary publication:
HIV-1 Nefs Are Cargo-Sensitive AP-1 Trimerization Switches in Tetherin Downregulation.
Morris KL, Buffalo CZ, Sturzel CM, Heusinger E, Kirchhoff F, Ren X, Hurley JH
Cell 174 659-671.e14 (2018)
PMID: 30053425

Function and Biology Details

Sample name: Structure of the cargo bound AP-1:Arf1:tetherin-Nef stable closed trimer
Ligands: GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION
Proteins: Structure of the cargo bound AP-1:Arf1:tetherin-Nef stable closed trimer, Bone marrow stromal antigen 2, Protein Nef chimera, AP-1 complex subunit beta-1, ADP-ribosylation factor 1, AP-1 complex subunit gamma-1, AP-1 complex subunit mu-1, AP-1 complex subunit sigma-3

Experimental Information Details

Resolution: 6.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)