6cri

Electron Microscopy
6.8Å resolution

Structure of the cargo bound AP-1:Arf1:tetherin-Nef stable closed trimer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Bone marrow stromal antigen 2; Protein Nef Chains: N, T, Y, Z, c, d
Molecule details ›
Chains: N, T, Y, Z, c, d
Length: 264 amino acids
Theoretical weight: 29.96 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q10589 (Residues: 2-21, 60-60; Coverage: 12%)
  • Canonical: P03404 (Residues: 1-32, 34-206; Coverage: 100%)
Gene names: BST2, nef
Sequence domains: Negative factor, (F-Protein) or Nef
AP-1 complex subunit beta-1 Chains: B, I, J
Molecule details ›
Chains: B, I, J
Length: 570 amino acids
Theoretical weight: 64.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q10567 (Residues: 14-583; Coverage: 60%)
Gene names: ADTB1, AP1B1, BAM22, CLAPB2
ADP-ribosylation factor 1 Chains: C, H, K, L, U, V
Molecule details ›
Chains: C, H, K, L, U, V
Length: 165 amino acids
Theoretical weight: 18.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P84077 (Residues: 17-181; Coverage: 91%)
Gene name: ARF1
AP-1 complex subunit gamma-1 Chains: G, Q, R
Molecule details ›
Chains: G, Q, R
Length: 585 amino acids
Theoretical weight: 66.4 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P22892 (Residues: 4-588; Coverage: 71%)
Gene names: Adtg, Ap1g1, Clapg1
Sequence domains: Adaptin N terminal region
AP-1 complex subunit mu-1 Chains: M, W, X
Molecule details ›
Chains: M, W, X
Length: 422 amino acids
Theoretical weight: 48.48 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P35585 (Residues: 2-423; Coverage: 100%)
Gene names: Ap1m1, Cltnm
Sequence domains: Adaptor complexes medium subunit family
AP-1 complex subunit sigma-3 Chains: S, a, b
Molecule details ›
Chains: S, a, b
Length: 142 amino acids
Theoretical weight: 17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96PC3 (Residues: 1-142; Coverage: 92%)
Gene name: AP1S3
Sequence domains: Clathrin adaptor complex small chain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.8Å
Relevant EMDB volumes: EMD-7563
Expression system: Escherichia coli