EMDB EMD-4936

Single particle reconstruction
3.4Å resolution

Structure of PTCH1 bound to a modified Hedgehog ligand ShhN-C24II

Map released:
Overview of EMD-4936
Source organisms:
Fitted atomic model: 6rmg
Related EM entry by publication: EMD-4939
Primary publication:
Structural basis of sterol recognition by human hedgehog receptor PTCH1.
Qi C, Di Minin G, Vercellino I, Wutz A, Korkhov VM
Sci Adv 5 eaaw6490-eaaw6490 (2019)
PMID: 31555730

Function and Biology Details

Sample name: Complex of PTCH1 with a modified Hedgehog ligand ShhN-C24II
Ligands: N-ACETYL-D-GLUCOSAMINE, CHOLESTEROL HEMISUCCINATE, ZINC ION
Proteins: Complex of PTCH1 with a modified Hedgehog ligand ShhN-C24II, Protein patched homolog 1 + GFP, Sonic hedgehog protein, Protein patched homolog 1,GFP-like fluorescent chromoprotein FP506, related, Sonic hedgehog protein

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)