EMDB EMD-4910

Single particle reconstruction
4.0Å resolution

E. coli DNA Gyrase - DNA binding and cleavage domain in State 1

Map released:
Last modified:
Overview of EMD-4910
Source organisms:
Fitted atomic model: 6rku
Related EM entries by publication: EMD-4909, EMD-4912, EMD-4913, EMD-4914, EMD-4915
Primary publication:
Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex.
Vanden Broeck A, Lotz C, Ortiz J, Lamour V
Nat Commun 10 4935-4935 (2019)
PMID: 31666516

Function and Biology Details

Sample name: DNA binding and cleavage domain of the E. coli DNA Gyrase in State 1
Ligand: (3~{R})-3-[[4-(3,4-dihydro-2~{H}-pyrano[2,3-c]pyridin-6-ylmethylamino)piperidin-1-yl]methyl]-1,4,7-triazatricyclo[6.3.1.0^{4,12}]dodeca-6,8(12),9-triene-5,11-dione
Nucleic acids: DNA Strand 1, DNA Strand 2
Proteins: DNA binding and cleavage domain of the E. coli DNA Gyrase in State 1, DNA Gyrase, DNA, DNA gyrase subunit A, DNA gyrase subunit B

Experimental Information Details

Resolution: 4.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)