6rku

Electron Microscopy
4Å resolution

E. coli DNA Gyrase - DNA binding and cleavage domain in State 1

Released:
Source organism: Escherichia coli K-12
Related structures: EMD-4910

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA gyrase subunit A Chains: A, C
Molecule details ›
Chains: A, C
Length: 875 amino acids
Theoretical weight: 97.09 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AES4 (Residues: 1-875; Coverage: 100%)
Gene names: JW2225, b2231, gyrA, hisW, nalA, parD
Sequence domains:
DNA gyrase subunit B Chains: B, D
Molecule details ›
Chains: B, D
Length: 804 amino acids
Theoretical weight: 90.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AES6 (Residues: 1-804; Coverage: 100%)
Gene names: JW5625, acrB, b3699, cou, gyrB, himB, hisU, nalC, parA, pcbA
Sequence domains:
DNA Strand 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 14 nucleotides
Theoretical weight: 4.29 KDa
DNA Strand 2 Chains: G, H
Molecule details ›
Chains: G, H
Length: 18 nucleotides
Theoretical weight: 5.51 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-4910
Expression system: Escherichia coli BL21(DE3)