EMDB EMD-4340

Helical reconstruction
3.93Å resolution

CryoEM structure of the MDA5-dsRNA filament with 87-degree helical twist

Map released:
Last modified:
Overview of EMD-4340
Source organisms:
Fitted atomic model: 6g1s
Related EM entries by publication: EMD-0012, EMD-0023, EMD-0024, EMD-0143, EMD-0145, EMD-4338, EMD-4341
Primary publication:
Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis.
Yu Q, Qu K, Modis Y
Mol. Cell 72 999-1012.e6 (2018)
PMID: 30449722

Function and Biology Details

Sample name: MDA5-dsRNA helical filament
Ligand: ZINC ION
Nucleic acids: RNA (5'-R(P*UP*CP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*AP*CP*G)-3'), RNA (5'-R(P*CP*GP*UP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*GP*A)-3')
Proteins: MDA5-dsRNA helical filament, MDA5, Double-stranded RNA from bacteriophage Phi6, Interferon-induced helicase C domain-containing protein 1

Experimental Information Details

Resolution: 3.93Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)