› 6g1s

Electron Microscopy
3.93Å resolution

CryoEM structure of the MDA5-dsRNA filament with 87-degree helical twist

Released:
DOI: 10.2210/pdb6g1s/pdb
PDB entry 6g1s coloured by chain, front view.
PDB entry 6g1s coloured by chain, side view.
PDB entry 6g1s coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis.
Yu Q, Qu K, Modis Y
Mol Cell 72 999-1012.e6 (2018)
PMID: 30449722
Related structures: EMD-4340

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185912 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Interferon-induced helicase C domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 693 amino acids
Theoretical weight: 79.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8R5F7 (Residues: 310-1020; Coverage: 68%)
Gene name: Ifih1
Sequence domains:
RNA (5'-R(P*UP*CP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*AP*CP*G)-3') Chain: X
Molecule details ›
Chain: X
Length: 15 nucleotides
Theoretical weight: 4.77 KDa
Source organism: Pseudomonas virus phi6
Expression system: Pseudomonas savastanoi pv. phaseolicola
RNA (5'-R(P*CP*GP*UP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*GP*A)-3') Chain: Y
Molecule details ›
Chain: Y
Length: 15 nucleotides
Theoretical weight: 4.81 KDa
Source organism: Pseudomonas virus phi6
Expression system: Pseudomonas savastanoi pv. phaseolicola

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.93Å
Relevant EMDB volumes: EMD-4340
Expression systems:
  • Escherichia coli BL21(DE3)
  • Pseudomonas savastanoi pv. phaseolicola

Quick links

Citations

15 review citations

The molecular mechanism of RIG-I activation and signaling.
Thoresen et al. (2021)
14 more

1 mention without citation

Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis.
Yu et al. (2018)