EMDB EMD-4093

Single particle reconstruction
3.9Å resolution

Entire ovine respiratory complex I, Combined Map

Map released:
Last modified:
Overview of EMD-4093
Source organism: Sheep [9940]
Fitted atomic model: 5lnk
Related EM entries by publication: EMD-4084, EMD-4090, EMD-4091
Primary publication:
Atomic structure of the entire mammalian mitochondrial complex I.
Fiedorczuk K, Letts JA, Degliesposti G, Kaszuba K, Skehel M, Sazanov LA
Nature 538 406-410 (2016)
PMID: 27595392

Function and Biology Details

Sample name: Mitochondrial complex I
Ligands: IRON/SULFUR CLUSTER, FLAVIN MONONUCLEOTIDE, FE2/S2 (INORGANIC) CLUSTER, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE, CARDIOLIPIN, ZINC ION, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate, 4'-PHOSPHOPANTETHEINE
Proteins: Mitochondrial complex I, Mitochondrial complex I, 51 kDa subunit, Mitochondrial complex I, 24 kDa subunit, Mitochondrial complex I, 75 kDa subunit, Mitochondrial complex I, 49 kDa subunit, Mitochondrial complex I, 30 kDa subunit, Mitochondrial complex I, PSST subunit, Mitochondrial complex I, TYKY subunit, Mitochondrial complex I, ND1 subunit, Mitochondrial complex I, ND2 subunit, Mitochondrial complex I, ND3 subunit, Mitochondrial complex I, ND4 subunit, Mitochondrial complex I, ND4L subunit, Mitochondrial complex I, ND5 subunit, Mitochondrial complex I, ND6 subunit, Mitochondrial complex I, 10 kDa subunit, Mitochondrial complex I, 13 kDa subunit, Mitochondrial complex I, 18 kDa subunit, Mitochondrial complex I, 39 kDa subunit, Mitochondrial complex I, B8 subunit, Mitochondrial complex I, B13 subunit, Mitochondrial complex I, B14 subunit, Mitochondrial complex I, B14.5a subunit, Mitochondrial complex I, B17.2 subunit, Acyl carrier protein, Mitochondrial complex I, 42 kDa subunit, Mitochondrial complex I, 15 kDa subunit, Mitochondrial complex I, B9 subunit, Mitochondrial complex I, B12 subunit, Mitochondrial complex I, B14.5b subunit, Mitochondrial complex I, B15 subunit, Mitochondrial complex I, B16.6 subunit, Mitochondrial complex I, B17 subunit, Mitochondrial complex I, B18 subunit, Mitochondrial complex I, B22 subunit, Mitochondrial complex I, AGGG subunit, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial, Mitochondrial complex I, ESSS subunit, Mitochondrial complex I, KFYI subunit, Mitochondrial complex I, MNLL subunit, Mitochondrial complex I, MWFE subunit, Mitochondrial complex I, PDSW subunit, Mitochondrial complex I, PGIV subunit, Mitochondrial complex I, SGDH subunit, Mitochondrial complex I, B14.7 subunit

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)