5lnk

Electron Microscopy
3.9Å resolution

Entire ovine respiratory complex I

Released:
Source organism: Ovis aries
Related structures: EMD-4093

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only 45-mer (preferred)
Entry contents:
44 distinct polypeptide molecules
Macromolecules (44 distinct):
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Chain: 1
Molecule details ›
Chain: 1
Length: 445 amino acids
Theoretical weight: 48.68 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PUX0 (Residues: 20-464; Coverage: 96%)
Gene name: NDUFV1
Sequence domains:
Structure domains:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Chain: 2
Molecule details ›
Chain: 2
Length: 217 amino acids
Theoretical weight: 23.84 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5NRY1 (Residues: 30-246; Coverage: 88%)
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Chain: 3
Molecule details ›
Chain: 3
Length: 704 amino acids
Theoretical weight: 77.03 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QB34 (Residues: 24-727; Coverage: 97%)
Gene name: NDUFS1
Sequence domains:
Complex I-49kD Chain: 4
Molecule details ›
Chain: 4
Length: 412 amino acids
Theoretical weight: 47.12 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PJ73 (Residues: 1-412; Coverage: 100%)
Gene name: NDUFS2
Sequence domains: Respiratory-chain NADH dehydrogenase, 49 Kd subunit
Structure domains: Cytochrome-c3 Hydrogenase; chain B
Complex I-30kD Chain: 5
Molecule details ›
Chain: 5
Length: 228 amino acids
Theoretical weight: 26.44 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PB27 (Residues: 39-266; Coverage: 86%)
Gene name: NDUFS3
Sequence domains: Respiratory-chain NADH dehydrogenase, 30 Kd subunit
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial Chain: 6
Molecule details ›
Chain: 6
Length: 179 amino acids
Theoretical weight: 20.1 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: P42026 (Residues: 38-216; Coverage: 83%)
Gene name: NDUFS7
Sequence domains: NADH ubiquinone oxidoreductase, 20 Kd subunit
Structure domains: Rossmann fold
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial Chain: 9
Molecule details ›
Chain: 9
Length: 176 amino acids
Theoretical weight: 20.22 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: P42028 (Residues: 37-212; Coverage: 83%)
Gene name: NDUFS8
Sequence domains:
NADH-ubiquinone oxidoreductase chain 1 Chain: H
Molecule details ›
Chain: H
Length: 318 amino acids
Theoretical weight: 35.88 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78747 (Residues: 1-318; Coverage: 100%)
Gene names: MT-ND1, MTND1, NADH1, ND1
Sequence domains: NADH dehydrogenase
NADH-ubiquinone oxidoreductase chain 2 Chain: N
Molecule details ›
Chain: N
Length: 347 amino acids
Theoretical weight: 39.15 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78748 (Residues: 1-347; Coverage: 100%)
Gene names: MT-ND2, MTND2, NADH2, ND2
Sequence domains:
NADH-ubiquinone oxidoreductase chain 3 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.11 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78753 (Residues: 1-115; Coverage: 100%)
Gene names: MT-ND3, MTND3, NADH3, ND3
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
NADH-ubiquinone oxidoreductase chain 4 Chain: M
Molecule details ›
Chain: M
Length: 459 amino acids
Theoretical weight: 52.06 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78755 (Residues: 1-459; Coverage: 100%)
Gene names: MT-ND4, MTND4, NADH4, ND4
Sequence domains:
NADH-ubiquinone oxidoreductase chain 4L Chain: K
Molecule details ›
Chain: K
Length: 98 amino acids
Theoretical weight: 10.84 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78754 (Residues: 1-98; Coverage: 100%)
Gene names: MT-ND4L, MTND4L, NADH4L, ND4L
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
NADH-ubiquinone oxidoreductase chain 5 Chain: L
Molecule details ›
Chain: L
Length: 599 amino acids
Theoretical weight: 67.55 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78756 (Residues: 1-599; Coverage: 99%)
Gene names: MT-ND5, MTND5, NADH5, ND5
Sequence domains:
NADH-ubiquinone oxidoreductase chain 6 Chain: J
Molecule details ›
Chain: J
Length: 175 amino acids
Theoretical weight: 19.13 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78757 (Residues: 1-175; Coverage: 100%)
Gene names: MT-ND6, MTND6, NADH6, ND6
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 6
NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial Chain: a
Molecule details ›
Chain: a
Length: 75 amino acids
Theoretical weight: 8.42 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: P25712 (Residues: 35-109; Coverage: 69%)
Gene name: NDUFV3
Sequence domains: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial Chain: b
Molecule details ›
Chain: b
Length: 96 amino acids
Theoretical weight: 10.65 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: P23934 (Residues: 29-124; Coverage: 77%)
Gene name: NDUFS6
Sequence domains: Zinc-finger domain
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Chain: c
Molecule details ›
Chain: c
Length: 133 amino acids
Theoretical weight: 15.38 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PE07 (Residues: 38-170; Coverage: 78%)
Gene name: NDUFS4
Sequence domains: ETC complex I subunit conserved region
Epimerase domain-containing protein Chain: d
Molecule details ›
Chain: d
Length: 338 amino acids
Theoretical weight: 38.66 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PI58 (Residues: 36-373; Coverage: 89%)
Gene name: NDUFA9
Sequence domains: NAD dependent epimerase/dehydratase family
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Chain: e
Molecule details ›
Chain: e
Length: 98 amino acids
Theoretical weight: 10.97 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QAH8 (Residues: 2-99; Coverage: 99%)
Gene name: NDUFA2
Sequence domains: Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain
Structure domains: Glutaredoxin
Complex I subunit B13 Chain: f
Molecule details ›
Chain: f
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PNX7 (Residues: 2-116; Coverage: 99%)
Gene name: NDUFA5
Sequence domains: ETC complex I subunit conserved region
Complex I-B14 Chain: g
Molecule details ›
Chain: g
Length: 127 amino acids
Theoretical weight: 14.92 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QC06 (Residues: 14-140; Coverage: 91%)
Gene name: NDUFA6
Sequence domains: Complex 1 protein (LYR family)
Complex I-B14.5a Chain: h
Molecule details ›
Chain: h
Length: 112 amino acids
Theoretical weight: 12.4 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P0I2 (Residues: 3-114; Coverage: 98%)
Gene name: NDUFA7
Sequence domains: NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a)
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Chain: i
Molecule details ›
Chain: i
Length: 145 amino acids
Theoretical weight: 17.12 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: B9VGZ9 (Residues: 1-145; Coverage: 100%)
Sequence domains: NADH ubiquinone oxidoreductase subunit NDUFA12
Acyl carrier protein Chains: X, j
Molecule details ›
Chains: X, j
Length: 88 amino acids
Theoretical weight: 10.12 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5NQT7 (Residues: 70-157; Coverage: 56%)
Gene name: NDUFAB1
Sequence domains: Phosphopantetheine attachment site
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Chain: k
Molecule details ›
Chain: k
Length: 320 amino acids
Theoretical weight: 36.8 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QBF5 (Residues: 36-355; Coverage: 90%)
Gene name: NDUFA10
Sequence domains: Deoxynucleoside kinase
Complex I-15 kDa Chain: l
Molecule details ›
Chain: l
Length: 105 amino acids
Theoretical weight: 12.47 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QFF9 (Residues: 2-106; Coverage: 99%)
Gene name: NDUFS5
Complex I-B9 Chain: m
Molecule details ›
Chain: m
Length: 83 amino acids
Theoretical weight: 9.19 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5NYM7 (Residues: 2-84; Coverage: 99%)
Gene name: NDUFA3
Sequence domains: NADH dehydrogenase 1 alpha subcomplex subunit 3
Complex I-B12 Chain: n
Molecule details ›
Chain: n
Length: 97 amino acids
Theoretical weight: 11.02 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q5T4 (Residues: 2-98; Coverage: 99%)
Gene name: NDUFB3
Sequence domains: NADH-ubiquinone oxidoreductase B12 subunit family
NADH dehydrogenase [ubiquinone] 1 subunit C2 Chain: o
Molecule details ›
Chain: o
Length: 120 amino acids
Theoretical weight: 14.23 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P9Q8 (Residues: 3-122; Coverage: 98%)
Gene name: NDUFC2
Sequence domains: NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2)
Complex I-B15 Chain: p
Molecule details ›
Chain: p
Length: 128 amino acids
Theoretical weight: 14.98 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QF73 (Residues: 3-130; Coverage: 99%)
Gene name: NDUFB4
Sequence domains: NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4)
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Chain: q
Molecule details ›
Chain: q
Length: 143 amino acids
Theoretical weight: 16.64 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: Q95KV7 (Residues: 2-144; Coverage: 99%)
Gene names: GRIM19, NDUFA13
Sequence domains: GRIM-19 protein
Complex I-B17 Chain: r
Molecule details ›
Chain: r
Length: 127 amino acids
Theoretical weight: 15.43 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PZE3 (Residues: 2-128; Coverage: 99%)
Gene name: NDUFB6
Complex I-B18 Chain: s
Molecule details ›
Chain: s
Length: 136 amino acids
Theoretical weight: 16.28 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P5V3 (Residues: 2-137; Coverage: 99%)
Gene name: NDUFB7
Sequence domains: NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7)
Complex I-B22 Chain: t
Molecule details ›
Chain: t
Length: 178 amino acids
Theoretical weight: 21.65 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PGA3 (Residues: 2-179; Coverage: 99%)
Gene name: NDUFB9
Sequence domains: Complex 1 protein (LYR family)
Complex I-AGGG Chain: u
Molecule details ›
Chain: u
Length: 72 amino acids
Theoretical weight: 8.5 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PVD7 (Residues: 37-108; Coverage: 67%)
Gene name: NDUFB2
Sequence domains: NADH dehydrogenase 1 beta subcomplex subunit 2
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Chain: v
Molecule details ›
Chain: v
Length: 158 amino acids
Theoretical weight: 18.82 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q1B0 (Residues: 29-186; Coverage: 85%)
Gene name: NDUFB8
Complex I-ESSS Chain: w
Molecule details ›
Chain: w
Length: 125 amino acids
Theoretical weight: 14.46 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PWF1 (Residues: 30-154; Coverage: 91%)
Gene name: NDUFB11
NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial Chain: x
Molecule details ›
Chain: x
Length: 49 amino acids
Theoretical weight: 5.81 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: Q02376 (Residues: 28-76; Coverage: 65%)
Gene name: NDUFC1
Sequence domains: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
Complex I-MNLL Chain: y
Molecule details ›
Chain: y
Length: 57 amino acids
Theoretical weight: 6.93 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QG39 (Residues: 2-58; Coverage: 98%)
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 Chain: z
Molecule details ›
Chain: z
Length: 70 amino acids
Theoretical weight: 8.21 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: Q02377 (Residues: 1-70; Coverage: 100%)
Gene name: NDUFA1
Sequence domains: NADH-ubiquinone oxidoreductase MWFE subunit
Complex I-PDSW Chain: Z
Molecule details ›
Chain: Z
Length: 175 amino acids
Theoretical weight: 20.88 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q5Y9 (Residues: 9-11, 12-170; Coverage: 95%)
Gene name: NDUFB10
Sequence domains: NADH-ubiquinone oxidoreductase subunit 10
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Chain: Y
Molecule details ›
Chain: Y
Length: 171 amino acids
Theoretical weight: 20.01 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PYA5 (Residues: 2-172; Coverage: 99%)
Gene name: NDUFA8
Sequence domains: CHCH domain
Complex I-SGDH Chain: W
Molecule details ›
Chain: W
Length: 143 amino acids
Theoretical weight: 16.71 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QHN8 (Residues: 47-189; Coverage: 76%)
Gene name: NDUFB5
Mitochondrial complex I, B14.7 subunit Chain: V
Molecule details ›
Chain: V
Length: 119 amino acids
Theoretical weight: 10.15 KDa
Source organism: Ovis aries

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN

Cofactor: Ligand NDP 1 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-4093