EMDB EMD-4038

Single particle reconstruction
3.83Å resolution

Cryo-EM structure of RecBCD+DNA complex revealing activated nuclease domain

Map released:
Last modified:
Overview of EMD-4038
Source organisms:
Fitted atomic model: 5ld2
Primary publication:
Mechanism for nuclease regulation in RecBCD.
Wilkinson M, Chaban Y, Wigley DB
Elife 5 - (2016)
PMID: 27644322

Function and Biology Details

Sample name: Ternary complex of RecBCD with forked DNA substrate and ADPNP
Ligands: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, MAGNESIUM ION
Nucleic acid: Fork-Hairpin DNA (70-MER)
Proteins: Ternary complex of RecBCD with forked DNA substrate and ADPNP, RecBCD enzyme subunit RecB,RecBCD enzyme subunit RecB,RecBCD enzyme subunit RecB, RecBCD enzyme subunit RecC, RecBCD enzyme subunit RecD

Experimental Information Details

Resolution: 3.83Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)