5ld2

Electron Microscopy
3.83Å resolution

Cryo-EM structure of RecBCD+DNA complex revealing activated nuclease domain

Released:
Source organism: Escherichia coli K-12
Primary publication:
Mechanism for nuclease regulation in RecBCD.
OpenAccess logo Elife 5 (2016)
PMID: 27644322
Related structures: EMD-4038

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (4 distinct):
RecBCD enzyme subunit RecB Chain: B
Molecule details ›
Chain: B
Length: 1181 amino acids
Theoretical weight: 133.72 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P08394 (Residues: 1-1180; Coverage: 100%)
Gene names: JW2788, b2820, ior, recB, rorA
Sequence domains:
Structure domains:
RecBCD enzyme subunit RecC Chain: C
Molecule details ›
Chain: C
Length: 1122 amino acids
Theoretical weight: 128.97 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P07648 (Residues: 1-1122; Coverage: 100%)
Gene names: JW2790, b2822, recC
Sequence domains:
Structure domains:
RecBCD enzyme subunit RecD Chain: D
Molecule details ›
Chain: D
Length: 609 amino acids
Theoretical weight: 67.05 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P04993 (Residues: 2-608; Coverage: 100%)
Gene names: JW2787, b2819, hopE, recD
Sequence domains:
Fork-Hairpin DNA (70-MER) Chain: X
Molecule details ›
Chain: X
Length: 70 nucleotides
Theoretical weight: 21.44 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.83Å
Relevant EMDB volumes: EMD-4038
Expression system: Escherichia coli