EMDB EMD-3727

Single particle reconstruction
4.4Å resolution

RNA polymerase I pre-initiation complex

Map released:
Last modified:
Overview of EMD-3727
Source organisms:
Fitted atomic model: 5oa1
Related EM entry by publication: EMD-3728, EMD-3729
Primary publication:
Structural insights into transcription initiation by yeast RNA polymerase I.
Sadian Y, Tafur L, Kosinski J, Jakobi AJ, Wetzel R, Buczak K, Hagen WJ, Beck M, Sachse C, Muller CW
EMBO J. 36 2698-2709 (2017)
PMID: 28739580

Function and Biology Details

Sample name: RNA polymerase I pre-initiation complex with Core Factor, Rrn3 and transcription scaffold
Nucleic acids: non-template DNA strand, template DNA strand
Proteins: RNA polymerase I pre-initiation complex with Core Factor, Rrn3 and transcription scaffold, RNA polymerase I pre-initiation complex with Core Factor, Rrn3 and transcription scaffold, RNA polymerase I pre-initiation complex with Core Factor, Rrn3 and transcription scaffold, RPA1_YEAST DNA-directed RNA polymerase I subunit RPA190, RPA2_YEAST DNA-directed RNA polymerase I subunit RPA135, RPA49_YEAST DNA-directed RNA polymerase I subunit, RPA43_YEAST DNA-directed RNA polymerase I subunit, RPAC1_YEAST DNA-directed RNA polymerases I and III subunit RPAC1, RPA34_YEAST DNA-directed RNA polymerase I subunit RPA34, RPAB1_YEAST DNA-directed RNA polymerases I, II, and III subunit RPABC1, RPAB2_YEAST DNA-directed RNA polymerases I, II, and III subunit RPABC2, RPAC2_YEAST DNA-directed RNA polymerases I and III subunit RPAC2, RPAB3_YEAST DNA-directed RNA polymerases I, II, and III subunit RPABC3, RPA14_YEAST DNA-directed RNA polymerase I subunit RPA14, RPA12_YEAST DNA-directed RNA polymerase I subunit RPA12, RPAB4_YEAST DNA-directed RNA polymerases I, II, and III subunit RPABC4, RPAB5_YEAST DNA-directed RNA polymerases I, II, and III subunit RPABC5, RRN3_YEAST RNA polymerase I-specific transcription initiation factor RRN3, RRN6_YEAST RNA polymerase I-specific transcription initiation factor RRN6, RRN7_YEAST RNA polymerase I-specific transcription initiation factor RRN7, RRN11_YEAST RNA polymerase I-specific transcription initiation factor RRN11

Experimental Information Details

Resolution: 4.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)