5oa1

Electron Microscopy
4.4Å resolution

RNA polymerase I pre-initiation complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 34-mer (preferred)
Entry contents:
29 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (31 distinct):
DNA-directed RNA polymerase I subunit RPA190 Chain: A
Molecule details ›
Chain: A
Length: 1664 amino acids
Theoretical weight: 186.68 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P10964 (Residues: 1-1664; Coverage: 100%)
Gene names: O6276, RPA1, RPA190, RRN1, YOR341W
Sequence domains:
DNA-directed RNA polymerase I subunit RPA135 Chain: B
Molecule details ›
Chain: B
Length: 1203 amino acids
Theoretical weight: 135.91 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P22138 (Residues: 1-1203; Coverage: 100%)
Gene names: RPA135, RPA2, RRN2, SRP3, YP9531.03C, YPR010C
Sequence domains:
DNA-directed RNA polymerases I and III subunit RPAC1 Chain: C
Molecule details ›
Chain: C
Length: 335 amino acids
Theoretical weight: 37.73 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P07703 (Residues: 1-335; Coverage: 100%)
Gene names: P8283.18, RPC40, RPC5, YPR110C
Sequence domains:
DNA-directed RNA polymerase I subunit RPA14 Chain: D
Molecule details ›
Chain: D
Length: 137 amino acids
Theoretical weight: 14.6 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P50106 (Residues: 1-137; Coverage: 100%)
Gene names: RPA14, YD8358.11, YDR156W
Sequence domains: Yeast RNA polymerase I subunit RPA14
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
Molecule details ›
Chain: E
Length: 215 amino acids
Theoretical weight: 25.12 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P20434 (Residues: 1-215; Coverage: 100%)
Gene names: RPA7, RPB5, RPC9, YBR1204, YBR154C
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
Molecule details ›
Chain: F
Length: 155 amino acids
Theoretical weight: 17.93 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P20435 (Residues: 1-155; Coverage: 100%)
Gene names: P9677.8, RPB6, RPO26, YPR187W
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerase I subunit RPA43 Chain: G
Molecule details ›
Chain: G
Length: 326 amino acids
Theoretical weight: 36.26 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P46669 (Residues: 1-326; Coverage: 100%)
Gene names: O6271, RPA43, RRN12, YOR340C
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
Molecule details ›
Chain: H
Length: 146 amino acids
Theoretical weight: 16.53 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P20436 (Residues: 1-146; Coverage: 100%)
Gene names: RPB8, YOR224C, YOR50-14
Sequence domains: RNA polymerase Rpb8
DNA-directed RNA polymerase I subunit RPA12 Chain: I
Molecule details ›
Chain: I
Length: 125 amino acids
Theoretical weight: 13.68 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32529 (Residues: 1-125; Coverage: 100%)
Gene names: J1747, RPA12, RRN4, YJR063W
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
Molecule details ›
Chain: J
Length: 70 amino acids
Theoretical weight: 8.29 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P22139 (Residues: 1-70; Coverage: 100%)
Gene names: RPB10, YOR210W
Sequence domains: RNA polymerases N / 8 kDa subunit
DNA-directed RNA polymerases I and III subunit RPAC2 Chain: K
Molecule details ›
Chain: K
Length: 142 amino acids
Theoretical weight: 16.17 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P28000 (Residues: 1-142; Coverage: 100%)
Gene names: N1937, RPC19, YNL113W
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
Molecule details ›
Chain: L
Length: 70 amino acids
Theoretical weight: 7.73 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40422 (Residues: 1-70; Coverage: 100%)
Gene names: RPB12, RPC10, YHR143BW, YHR143W-A
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
DNA-directed RNA polymerase I subunit RPA49 Chain: M
Molecule details ›
Chain: M
Length: 415 amino acids
Theoretical weight: 46.72 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q01080 (Residues: 1-415; Coverage: 100%)
Gene names: N0880, RPA49, RRN13, YNL248C
Sequence domains: A49-like RNA polymerase I associated factor
DNA-directed RNA polymerase I subunit RPA34 Chain: N
Molecule details ›
Chain: N
Length: 233 amino acids
Theoretical weight: 26.93 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P47006 (Residues: 1-233; Coverage: 100%)
Gene names: J0637, RPA34, YJL148W
Sequence domains: DNA-directed RNA polymerase I subunit RPA34.5
RNA polymerase I-specific transcription initiation factor RRN3 Chain: O
Molecule details ›
Chain: O
Length: 627 amino acids
Theoretical weight: 72.46 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P36070 (Residues: 1-627; Coverage: 100%)
Gene names: RRN3, YKL125W
Sequence domains: RNA polymerase I specific transcription initiation factor RRN3
RNA polymerase I-specific transcription initiation factor RRN7 Chain: U
Molecule details ›
Chain: U
Length: 514 amino acids
Theoretical weight: 60.44 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40992 (Residues: 1-514; Coverage: 100%)
Gene names: J1273, RRN7, YJL025W
Sequence domains: Zinc-finger of RNA-polymerase I-specific TFIIB, Rrn7
RNA polymerase I-specific transcription initiation factor RRN6 Chain: V
Molecule details ›
Chain: V
Length: 894 amino acids
Theoretical weight: 102.16 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32786 (Residues: 1-894; Coverage: 100%)
Gene names: RRN6, YBL014C, YBL0311, YBL0312
Sequence domains: RNA polymerase I-specific transcription-initiation factor
RNA polymerase I-specific transcription initiation factor RRN11 Chain: W
Molecule details ›
Chain: W
Length: 507 amino acids
Theoretical weight: 59.33 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04712 (Residues: 1-507; Coverage: 100%)
Gene names: RRN11, YM9827.09C, YML043C
Sequence domains: RNA polymerase I specific initiation factor
ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA Chain: 1
Molecule details ›
Chain: 1
Length: 13 amino acids
Theoretical weight: 942 Da
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA Chain: 2
Molecule details ›
Chain: 2
Length: 19 amino acids
Theoretical weight: 1.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA Chains: 3, 9
Molecule details ›
Chains: 3, 9
Length: 9 amino acids
Theoretical weight: 658 Da
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA Chain: 4
Molecule details ›
Chain: 4
Length: 24 amino acids
Theoretical weight: 1.72 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA Chains: 5, Q
Molecule details ›
Chains: 5, Q
Length: 16 amino acids
Theoretical weight: 1.16 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA Chains: 6, 8
Molecule details ›
Chains: 6, 8
Length: 10 amino acids
Theoretical weight: 729 Da
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA Chain: 7
Molecule details ›
Chain: 7
Length: 25 amino acids
Theoretical weight: 1.79 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA Chain: P
Molecule details ›
Chain: P
Length: 22 amino acids
Theoretical weight: 1.58 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA Chain: Z
Molecule details ›
Chain: Z
Length: 15 amino acids
Theoretical weight: 1.08 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA Chain: Y
Molecule details ›
Chain: Y
Length: 12 amino acids
Theoretical weight: 871 Da
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA Chain: R
Molecule details ›
Chain: R
Length: 27 amino acids
Theoretical weight: 1.94 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
DNA (49-MER) Chain: S
Molecule details ›
Chain: S
Length: 70 nucleotides
Theoretical weight: 22 KDa
DNA (56-MER) Chain: T
Molecule details ›
Chain: T
Length: 70 nucleotides
Theoretical weight: 21.22 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-3727
Expression system: Escherichia coli