EMDB EMD-22283

Single particle reconstruction
3.1Å resolution

cryo-EM of human GLP-1R bound to non-peptide agonist LY3502970

Map released:
Last modified:
Overview of EMD-22283
Source organisms:
Fitted atomic model: 6xox
Primary publication:
Structural basis for GLP-1 receptor activation by LY3502970, an orally active nonpeptide agonist.
Kawai T, Sun B, Yoshino H, Feng D, Suzuki Y, Fukazawa M, Nagao S, Wainscott DB, Showalter AD, Droz BA, Kobilka TS, Coghlan MP, Willard FS, Kawabe Y, Kobilka BK, Sloop KW
Proc.Natl.Acad.Sci.USA - (2020)
PMID: 33177239

Function and Biology Details

Sample name: Cryo-EM structure of the Glucagon-like peptide-1 receptor in complex with G protein and a non-peptide agonist LY3502970
Ligand: 3-[(1S,2S)-1-(5-[(4S)-2,2-dimethyloxan-4-yl]-2-{(4S)-2-(4-fluoro-3,5-dimethylphenyl)-3-[3-(4-fluoro-1-methyl-1H-indazol-5-yl)-2-oxo-2,3-dihydro-1H-imidazol-1-yl]-4-methyl-2,4,6,7-tetrahydro-5H-pyrazolo[4,3-c]pyridine-5-carbonyl}-1H-indol-1-yl)-2-methylcyclopropyl]-1,2,4-oxadiazol-5(4H)-one
Proteins: Cryo-EM structure of the Glucagon-like peptide-1 receptor in complex with G protein and a non-peptide agonist LY3502970, Glucagon-like peptide 1 receptor, Trimeric stimulatory G protein, Nanobody 35, Single-chain variable fragment 16, Alpha subunit of Gs with N-terminus swapped with equivalent residues in Gi,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, single-chain variable fragment scFv16, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Nanobody 35, Glucagon-like peptide 1 receptor

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)