6xox

Electron Microscopy
3.1Å resolution

cryo-EM of human GLP-1R bound to non-peptide agonist LY3502970

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 43.39 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 27-394; Coverage: 90%)
  • Canonical: Q5JWF2 (Residues: 669-669; Coverage: 0%)
Gene names: GNAS, GNAS1, GSP
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
single-chain variable fragment scFv16 Chain: E
Molecule details ›
Chain: E
Length: 259 amino acids
Theoretical weight: 27.78 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 160 amino acids
Theoretical weight: 17.35 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Glucagon-like peptide 1 receptor Chain: R
Molecule details ›
Chain: R
Length: 445 amino acids
Theoretical weight: 51.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43220 (Residues: 24-422; Coverage: 91%)
Gene name: GLP1R
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-22283
Expression systems:
  • Trichoplusia ni
  • Spodoptera frugiperda
  • Escherichia coli