EMDB EMD-22237

Single particle reconstruction
3.1Å resolution

Cryo-EM structure of EcmrR-DNA complex in EcmrR-RPitc-3nt

Map released:
Overview of EMD-22237
Source organism: Escherichia coli [562]
Fitted atomic model: 6xla
To be published

Function and Biology Details

Sample name: EcmrR-spacer DNA complex in EcmrR-RNAP-promoter initial transcribing complex with 3-nt RNA transcript (EcmrR-RPitc-3nt)
Ligand: TETRAPHENYLANTIMONIUM ION
Nucleic acids: synthetic non-template strand DNA (54-MER), synthetic template strand DNA (54-MER)
Proteins: EcmrR-spacer DNA complex in EcmrR-RNAP-promoter initial transcribing complex with 3-nt RNA transcript (EcmrR-RPitc-3nt), MerR family transcriptional regulator EcmrR

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)