› 6xla

Electron Microscopy
3.1Å resolution

Cryo-EM structure of EcmrR-DNA complex in EcmrR-RPitc-3nt

Released:
DOI: 10.2210/pdb6xla/pdb
PDB entry 6xla coloured by chain, front view.
PDB entry 6xla coloured by chain, side view.
PDB entry 6xla coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural visualization of transcription activated by a multidrug-sensing MerR family regulator.
Yang Y, Liu C, Zhou W, Shi W, Chen M, Zhang B, Schatz DG, Hu Y, Liu B
Nat Commun 12 2702 (2021)
PMID: 33976201
Related structures: EMD-22237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-120963 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
MerR family transcriptional regulator EcmrR Chains: G, H
Molecule details ›
Chains: G, H
Length: 268 amino acids
Theoretical weight: 31.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
synthetic non-template strand DNA (54-MER) Chain: N
Molecule details ›
Chain: N
Length: 54 nucleotides
Theoretical weight: 16.72 KDa
Source organism: Escherichia coli
Expression system: Not provided
synthetic template strand DNA (54-MER) Chain: T
Molecule details ›
Chain: T
Length: 54 nucleotides
Theoretical weight: 16.63 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand 118 2 x 118
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-22237
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 citation in other articles

Structural basis for intrinsic transcription termination.
You et al. (2023)
13 more

3 mentions without citation

Bacterial MerR family transcription regulators: activationby distortion.
Fang et al. (2022)
2 more