EMDB EMD-21308

Single particle reconstruction
3.2Å resolution

Structure of the SpCas9 DNA adenine base editor - ABE8e

Map released:
Last modified:
Overview of EMD-21308
Source organisms:
Fitted atomic model: 6vpc
Primary publication:
DNA capture by a CRISPR-Cas9-guided adenine base editor.
Lapinaite A, Knott GJ, Palumbo CM, Lin-Shiao E, Richter MF, Zhao KT, Beal PA, Liu DR, Doudna JA
Science 369 566-571 (2020)
PMID: 32732424

Function and Biology Details

Sample name: CRISPR-Cas9 ABE8e in a substrate-bound state
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: Cas9 (SpCas9) single-guide RNA (sgRNA), DNA target strand (TS), DNA non-target strand (NTS)
Proteins: CRISPR-Cas9 ABE8e in a substrate-bound state, CRISPR-associated endonuclease Cas9, t-RNA adenine deaminase A v8e (TadA-8e)

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: OTHER