6vpc

Electron Microscopy
3.2Å resolution

Structure of the SpCas9 DNA adenine base editor - ABE8e

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
CRISPR-associated endonuclease Cas9 Chain: B
Molecule details ›
Chain: B
Length: 1361 amino acids
Theoretical weight: 157.87 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A4U7H5M9 (Residues: 4-1364; Coverage: 100%)
Gene names: G3M98_08885, GTK50_03670, SAMEA3919037_00489, cas9, csn1
Sequence domains:
t-RNA adenine deaminase A v8e (TadA-8e) Chains: E, F
Molecule details ›
Chains: E, F
Length: 227 amino acids
Theoretical weight: 24.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
DNA target strand (TS) Chain: C
Molecule details ›
Chain: C
Length: 50 nucleotides
Theoretical weight: 15.39 KDa
DNA non-target strand (NTS) Chain: D
Molecule details ›
Chain: D
Length: 50 nucleotides
Theoretical weight: 15.44 KDa
Cas9 (SpCas9) single-guide RNA (sgRNA) Chain: A
Molecule details ›
Chain: A
Length: 82 nucleotides
Theoretical weight: 26.36 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-21308
Expression system: Escherichia coli