EMDB EMD-21301

Single particle reconstruction
3.7Å resolution

Cryo-EM structure of HTLV-1 instasome

Map released:
Overview of EMD-21301
Source organisms:
Fitted atomic model: 6voy
Primary publication:
Structural basis of host protein hijacking in human T-cell leukemia virus integration.
Bhatt V, Shi K, Salamango DJ, Moeller NH, Pandey KK, Bera S, Bohl TE, Kurniawan F, Orellana K, Zhang W, Grandgenett DP, Harris RS, Sundborger-Lunna AC, Aihara H
Nat Commun 11 3121-3121 (2020)
PMID: 32561747

Function and Biology Details

Sample name: HTLV1 intasome
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: DNA (5'-D(P*AP*CP*AP*CP*AP*CP*TP*TP*GP*AP*CP*TP*AP*GP*GP*GP*TP*G)-3'), DNA (25-MER), DNA (5'-D(P*AP*CP*AP*CP*AP*CP*TP*TP*GP*AP*CP*TP*AP*GP*GP*GP*TP*G)-3')
Proteins: HTLV1 intasome, DNA-binding protein 7d, Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)