EMDB EMD-21232

Single particle reconstruction
4.42Å resolution

BG505 SOSIP.v5.2 in complex with rhesus macaque Fab RM20E1 and PGT122 Fab

Map released:
Overview of EMD-21232
Source organisms:
Fitted atomic model: 6vlr
Primary publication:
Mapping the immunogenic landscape of native HIV-1 envelope trimers in non-human primates
Cottrell CA, van Schooten J, Bowman CA, Yuan M, Oyen D, Shin M, Morpurgo R, van der Woude P, van Breemen M, Torres JL, Patel R, Gross J, Sewall LM, Copps J, Ozorowski G, Sok D, Rakasz EG, Labranche C, Vigdorovich V, Christley S, Sather DN, Montefiori D, Moore JP, Burton DR, Wilson IA, Sanders RW, Ward AB, van Gils MJ
Biorxiv - (2020)

Function and Biology Details

Sample name: BG505 SOSIP.v5.2 in complex with rhesus macaque Fab RM20E1
Ligands: N-ACETYL-D-GLUCOSAMINE, BETA-D-MANNOSE, ALPHA-D-MANNOSE
Proteins: BG505 SOSIP.v5.2 in complex with rhesus macaque Fab RM20E1, Envelope glycoprotein gp120, BG505 SOSIPv5.2 gp41, PGT122 Fab Heavy Chain, PGT122 Fab Light Chain, RM20E1 Fab Heavy Chain, RM20E1 Fab Kappa Chain

Experimental Information Details

Resolution: 4.42Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC, RELION
Microscope: FEI TALOS ARCTICA
Detector: GATAN K2 SUMMIT (4k x 4k)