EMDB EMD-20487

Single particle reconstruction
3.34Å resolution

Human alpha3beta4 nicotinic acetylcholine receptor in complex with nicotine

Map released:
Last modified:
Overview of EMD-20487
Source organisms:
Fitted atomic model: 6pv7
Related EM entries by publication: EMD-20488, EMD-20489, EMD-20490
Primary publication:
Agonist Selectivity and Ion Permeation in the alpha 3 beta 4 Ganglionic Nicotinic Receptor.
Gharpure A, Teng J, Zhuang Y, Noviello CM, Walsh Jr RM, Cabuco R, Howard RJ, Zaveri NT, Lindahl E, Hibbs RE
Neuron - (2019)
PMID: 31488329

Function and Biology Details

Sample name: Human alpha3beta4 nicotinic receptor in complex with Fab fragments and bound to nicotine
Ligands: (S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE, N-ACETYL-D-GLUCOSAMINE, BETA-D-MANNOSE, ALPHA-D-MANNOSE, CHOLESTEROL HEMISUCCINATE, SODIUM ION, nonane, water
Proteins: Human alpha3beta4 nicotinic receptor in complex with Fab fragments and bound to nicotine, Human alpha3beta4 nicotinic acetylcholine receptor, Neuronal acetylcholine receptor subunit alpha-3, Neuronal acetylcholine receptor subunit beta-4, IgG2b/kappa antibody, IgG2b heavy chain, Kappa Fab light chain, Fusion protein of Neuronal acetylcholine receptor subunit alpha-3 and Soluble cytochrome b562, Fusion protein of Neuronal acetylcholine receptor subunit beta-4 and Soluble cytochrome b562, IgG2b Fab heavy chain, Kappa Fab light chain

Experimental Information Details

Resolution: 3.34Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)