6pv7

Electron Microscopy
3.34Å resolution

Human alpha3beta4 nicotinic acetylcholine receptor in complex with nicotine

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
Neuronal acetylcholine receptor subunit alpha-3 Chains: A, D
Molecule details ›
Chains: A, D
Length: 525 amino acids
Theoretical weight: 60.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P32297 (Residues: 32-378, 379-386, 387-398, 399-431, 434-505; Coverage: 100%)
Gene names: CHRNA3, NACHRA3
Sequence domains:
Neuronal acetylcholine receptor subunit beta-4 Chains: B, C, E
Molecule details ›
Chains: B, C, E
Length: 538 amino acids
Theoretical weight: 61.34 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P30926 (Residues: 22-363, 364-374, 375-379, 380-398, 399-416, 417-498; Coverage: 100%)
  • Best match: P30926-2 (Residues: 22-121)
Gene name: CHRNB4
Sequence domains:
IgG2b Fab heavy chain Chains: F, H
Molecule details ›
Chains: F, H
Length: 219 amino acids
Theoretical weight: 23.4 KDa
Source organism: Mus musculus
Kappa Fab light chain Chains: G, I
Molecule details ›
Chains: G, I
Length: 213 amino acids
Theoretical weight: 23.4 KDa
Source organism: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.34Å
Relevant EMDB volumes: EMD-20487
Expression system: Homo sapiens