EMDB EMD-20455

Single particle reconstruction
4.6Å resolution

Cryo-EM structure of HzTransib/nicked TIR substrate DNA hairpin forming complex (HFC)

Map released:
Overview of EMD-20455
Source organism: Helicoverpa zea [7113]
Fitted atomic model: 6pqx
Primary publication:
Structures of a RAG-like transposase during cut-and-paste transposition
Liu C, Yang Y, Schatz DG
Nature - (2019)

Function and Biology Details

Sample name: Hairpin forming complex of HzTransib with nicked TIR substrate DNA
Ligand: CALCIUM ION
Nucleic acids: DNA (5'-D(P*TP*CP*TP*GP*GP*CP*CP*TP*AP*GP*AP*TP*CP*T)-3'), DNA (5'-D(P*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3'), TIR substrate DNA transferred strand
Proteins: Hairpin forming complex of HzTransib with nicked TIR substrate DNA, DNA-mediated transposase

Experimental Information Details

Resolution: 4.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)