6pqx

Electron Microscopy
4.6Å resolution

Cryo-EM structure of HzTransib/nicked TIR substrate DNA hairpin forming complex (HFC)

Released:
Source organism: Helicoverpa zea
Related structures: EMD-20455

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Putative DNA-mediated transposase Chains: A, E
Molecule details ›
Chains: A, E
Length: 497 amino acids
Theoretical weight: 56.58 KDa
Source organism: Helicoverpa zea
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: B0F0C5 (Residues: 17-507; Coverage: 93%)
DNA (5'-D(P*TP*CP*TP*GP*GP*CP*CP*TP*AP*GP*AP*TP*CP*T)-3') Chains: B, F
Molecule details ›
Chains: B, F
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Helicoverpa zea
Expression system: Not provided
DNA (5'-D(P*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3') Chains: C, G
Molecule details ›
Chains: C, G
Length: 16 nucleotides
Theoretical weight: 4.96 KDa
Source organism: Helicoverpa zea
Expression system: Not provided
TIR substrate DNA transferred strand Chains: D, H
Molecule details ›
Chains: D, H
Length: 32 nucleotides
Theoretical weight: 9.79 KDa
Source organism: Helicoverpa zea
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-20455
Expression systems:
  • Spodoptera frugiperda
  • Not provided