EMDB EMD-20178

Single particle reconstruction
4.4Å resolution

Modified BG505 SOSIP-based immunogen RC1 in complex with the elicited V3-glycan patch antibody Ab275MUR

Map released:
Overview of EMD-20178
Source organisms:
Fitted atomic model: 6orq
Related EM entries by publication: EMD-20175, EMD-20176, EMD-20177
Primary publication:
Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques.
Escolano A, Gristick HB, Abernathy ME, Merkenschlager J, Gautam R, Oliveira TY, Pai J, West Jr AP, Barnes CO, Cohen AA, Wang H, Golijanin J, Yost D, Keeffe JR, Wang Z, Zhao P, Yao KH, Bauer J, Nogueira L, Gao H, Voll AV, Montefiori DC, Seaman MS, Gazumyan A, Silva M, McGuire AT, Stamatatos L, Irvine DJ, Wells L, Martin MA, Bjorkman PJ, Nussenzweig MC
Nature - (2019)
PMID: 31142836

Function and Biology Details

Sample name: Complex of RC1 variant of BG505 SOSIP.664 trimer with three Ab275MUR Fabs and three 8ANC195 Fabs
Ligands: N-ACETYL-D-GLUCOSAMINE, BETA-D-MANNOSE, ALPHA-D-MANNOSE
Proteins: Complex of RC1 variant of BG505 SOSIP.664 trimer with three Ab275MUR Fabs and three 8ANC195 Fabs, RC1 variant of BG505 SOSIP.664 trimer, Ab275MUR Fab, RC1 variant of HIV-1 Env glycoprotein gp41, RC1 variant of HIV-1 Env glycoprotein gp120, Ab275MUR antibody Fab heavy chain, Ab275MUR antibody Fab light chain

Experimental Information Details

Resolution: 4.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C3
Reconstruction software: cryoSPARC
Microscope: FEI TALOS ARCTICA
Detector: FEI FALCON III (4k x 4k)