EMDB EMD-20132

Single particle reconstruction
3.9Å resolution

CryoEM structure of the LbCas12a-crRNA-AcrVA4-DNA complex

Map released:
Last modified:
Overview of EMD-20132
Source organisms:
Fitted atomic model: 6omv
Related EM entries by publication: EMD-0445, EMD-0446, EMD-0447, EMD-0449, EMD-20182, EMD-9398
Primary publication:
Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins.
Zhang H, Li Z, Daczkowski CM, Gabel C, Mesecar AD, Chang L
Cell Host Microbe 25 815- (2019)
PMID: 31155345

Function and Biology Details

Sample name: DNA complex
Ligands: MAGNESIUM ION, water
Nucleic acids: DNA (5'-D(P*TP*AP*AP*TP*TP*TP*CP*CP*TP*AP*AP*AP*GP*GP*AP*CP*G)-3'), DNA (5'-D(*CP*GP*TP*CP*CP*TP*TP*TP*AP*GP*GP*A)-3'), RNA
Proteins: DNA complex, AcrVA4, Cpf1

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)