6omv

Electron Microscopy
3.9Å resolution

CryoEM structure of the LbCas12a-crRNA-AcrVA4-DNA complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
AcrVA4 Chains: A, C
Molecule details ›
Chains: A, C
Length: 234 amino acids
Theoretical weight: 27.37 KDa
Source organism: Moraxella bovoculi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0U2APF4 (Residues: 1-234; Coverage: 100%)
Gene name: AAX07_09545
Cpf1 Chain: B
Molecule details ›
Chain: B
Length: 1227 amino acids
Theoretical weight: 143.75 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A182DWE3 (Residues: 3-1228; Coverage: 100%)
Sequence domains:
DNA (5'-D(P*TP*AP*AP*TP*TP*TP*CP*CP*TP*AP*AP*AP*GP*GP*AP*CP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.21 KDa
DNA (5'-D(*CP*GP*TP*CP*CP*TP*TP*TP*AP*GP*GP*A)-3') Chain: F
Molecule details ›
Chain: F
Length: 12 nucleotides
Theoretical weight: 3.65 KDa
RNA Chain: G
Molecule details ›
Chain: G
Length: 40 nucleotides
Theoretical weight: 12.88 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-20132
Expression system: Escherichia coli BL21(DE3)