EMDB EMD-11821

Single particle reconstruction
3.4Å resolution

Intermediate assembly of the Large subunit from Leishmania major mitochondrial ribosome

Map released:
Overview of EMD-11821
Source organism: Leishmania tarentolae [5689]
Fitted atomic model: 7am2
Primary publication:
Structure of the mature kinetoplastids mitoribosome and insights into its large subunit biogenesis.
Soufari H, Waltz F, Parrot C, Durrieu-Gaillard S, Bochler A, Kuhn L, Sissler M, Hashem Y
Proc.Natl.Acad.Sci.USA 117 29851-29861 (2020)
PMID: 33168716

Function and Biology Details

Sample name: Leishmania mitochondrial ribosome
Ligands: GUANOSINE-5'-TRIPHOSPHATE, ADENOSINE-5'-TRIPHOSPHATE
Nucleic acids: Ribosomal RNA, R1, R2, R5
Proteins: Leishmania mitochondrial ribosome, Ribosomal protein L3-like protein, uL4m, RIBOSOMAL_L9 domain-containing protein, Putative ribosomal protein L11, 50S ribosomal protein L13-like protein, Ribosomal_L18e/L15P domain-containing protein, Putative 50S ribosomal protein L17, bL19m, bL20m, bL21m, uL22m, uL23m, uL24m, bL28m, uL29m, uL30m, bL32m, bL35m, mL41, mL94, TRUD domain-containing protein, UA, mL95, RNA uridylyltransferase, mL67, mL71, mL81, mL98, Putative ribosomal protein L14, mL86, mL87, mL53, mL63, mL68, mL80, mL74, L51_S25_CI-B8 domain-containing protein, mL42, mL52,mL52, mL93, mL75, mL85, mL100, mL49, mL89, Peptidyl-prolyl cis-trans isomerase, mL72, mL84, mL76, mL99, mL88, mL70, mL59/64, Peptidyl-prolyl cis-trans isomerase, LIM zinc-binding domain-containing protein, DNAj-like protein, SpoU_methylase domain-containing protein, Pseudouridylate synthase-like protein, Acyl carrier protein, L0R8F8, G domain-containing protein, GTPase Der, DEAD/DEAH box helicase-like protein, G domain-containing protein, mL78, U6, U1, U3, U4, U5, mL78, U2, U8

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TALOS ARCTICA
Detector: FEI FALCON III (4k x 4k)