7am2

Electron Microscopy
3.4Å resolution

Intermediate assembly of the Large subunit from Leishmania major mitochondrial ribosome

Released:
Source organism: Leishmania tarentolae
Related structures: EMD-11821

Function and Biology Details

Reactions catalysed:
UTP + RNA(n) = diphosphate + RNA(n+1)
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 78-mer (preferred)
Entry contents:
73 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (77 distinct):
Ribosomal protein L3-like protein Chain: A
Molecule details ›
Chain: A
Length: 467 amino acids
Theoretical weight: 54.03 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: E9ADK5 (Residues: 1-467; Coverage: 100%)
Gene name: LMJF_29_0030
Sequence domains: Ribosomal protein L3
uL4m Chain: B
Molecule details ›
Chain: B
Length: 436 amino acids
Theoretical weight: 50 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QGU6 (Residues: 1-436; Coverage: 100%)
Gene name: LMJF_11_1360
Sequence domains: Ribosomal protein L4/L1 family
RIBOSOMAL_L9 domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 262 amino acids
Theoretical weight: 30.51 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QIE5 (Residues: 1-262; Coverage: 100%)
Gene name: LMJF_08_0240
Sequence domains: Ribosomal protein L9, N-terminal domain
Putative ribosomal protein L11 Chain: E
Molecule details ›
Chain: E
Length: 346 amino acids
Theoretical weight: 40.27 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: E9ACT9 (Residues: 1-346; Coverage: 100%)
Gene name: LMJF_27_2220
Sequence domains:
50S ribosomal protein L13-like protein Chain: F
Molecule details ›
Chain: F
Length: 171 amino acids
Theoretical weight: 20.5 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q2H6 (Residues: 1-171; Coverage: 100%)
Gene name: LMJF_34_3680
Sequence domains: Ribosomal protein L13
Ribosomal_L18e/L15P domain-containing protein Chain: G
Molecule details ›
Chain: G
Length: 374 amino acids
Theoretical weight: 43.57 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QF19 (Residues: 1-374; Coverage: 100%)
Gene name: LMJF_16_0010
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Putative 50S ribosomal protein L17 Chain: I
Molecule details ›
Chain: I
Length: 305 amino acids
Theoretical weight: 35.83 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QF04 (Residues: 1-305; Coverage: 100%)
Gene name: LMJF_16_0160
Sequence domains: Ribosomal protein L17
bL19m Chain: J
Molecule details ›
Chain: J
Length: 144 amino acids
Theoretical weight: 16.7 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QCY7 (Residues: 1-144; Coverage: 100%)
Gene name: LMJF_20_0530
bL20m Chain: K
Molecule details ›
Chain: K
Length: 194 amino acids
Theoretical weight: 22.44 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q192 (Residues: 1-194; Coverage: 100%)
Gene name: LMJF_36_3260
bL21m Chain: L
Molecule details ›
Chain: L
Length: 186 amino acids
Theoretical weight: 21.04 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QFQ5 (Residues: 1-186; Coverage: 100%)
Gene name: LMJF_14_0780
Sequence domains: Ribosomal prokaryotic L21 protein
uL22m Chain: M
Molecule details ›
Chain: M
Length: 279 amino acids
Theoretical weight: 32.28 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QBR0 (Residues: 1-279; Coverage: 100%)
Gene name: LMJF_22_0870
Sequence domains: Ribosomal protein L22p/L17e
uL23m Chain: N
Molecule details ›
Chain: N
Length: 252 amino acids
Theoretical weight: 28.84 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QA05 (Residues: 1-252; Coverage: 100%)
Gene name: LMJF_25_0890
uL24m Chain: O
Molecule details ›
Chain: O
Length: 476 amino acids
Theoretical weight: 53.62 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q9S7 (Residues: 1-476; Coverage: 100%)
Gene name: LMJF_25_1640
bL28m Chain: Q
Molecule details ›
Chain: Q
Length: 234 amino acids
Theoretical weight: 27.22 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q719 (Residues: 1-234; Coverage: 100%)
Gene name: LMJF_30_2750
uL29m Chain: R
Molecule details ›
Chain: R
Length: 480 amino acids
Theoretical weight: 54.24 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q9XZY7 (Residues: 1-480; Coverage: 100%)
Gene name: LMJF_04_0270
Sequence domains: Mitochondrial 39-S ribosomal protein L47 (MRP-L47)
uL30m Chain: S
Molecule details ›
Chain: S
Length: 409 amino acids
Theoretical weight: 45.56 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q9U0Z7 (Residues: 1-409; Coverage: 100%)
Gene name: LMJF_04_0860
bL32m Chain: T
Molecule details ›
Chain: T
Length: 83 amino acids
Theoretical weight: 9.87 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q2W9 (Residues: 1-83; Coverage: 100%)
Gene name: LMJF_34_2310
bL35m Chain: V
Molecule details ›
Chain: V
Length: 151 amino acids
Theoretical weight: 18.39 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QCK6 (Residues: 1-151; Coverage: 100%)
Gene name: LMJF_21_0100
mL41 Chain: Z
Molecule details ›
Chain: Z
Length: 197 amino acids
Theoretical weight: 22.67 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q152 (Residues: 1-197; Coverage: 100%)
Gene name: LMJF_36_3653
mL94 Chain: BA
Molecule details ›
Chain: BA
Length: 167 amino acids
Theoretical weight: 17.89 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: E9ACP5 (Residues: 1-167; Coverage: 100%)
Gene name: LMJF_04_0045
TRUD domain-containing protein Chain: CA
Molecule details ›
Chain: CA
Length: 618 amino acids
Theoretical weight: 68.4 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QGU5 (Residues: 1-618; Coverage: 100%)
Gene name: LMJF_11_1370
UA Chain: UA
Molecule details ›
Chain: UA
Length: 203 amino acids
Theoretical weight: 14.45 KDa
Source organism: Leishmania tarentolae
mL95 Chain: BB
Molecule details ›
Chain: BB
Length: 156 amino acids
Theoretical weight: 18.69 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q4D6 (Residues: 1-156; Coverage: 100%)
Gene name: LMJF_33_0690
RNA uridylyltransferase Chain: CB
Molecule details ›
Chain: CB
Length: 202 amino acids
Theoretical weight: 23.36 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q9NE59 (Residues: 1-202; Coverage: 100%)
Gene names: L5213T.03, LMJF_30_2120
Sequence domains: Ribosomal silencing factor during starvation
mL67 Chain: BK
Molecule details ›
Chain: BK
Length: 893 amino acids
Theoretical weight: 97.7 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: E9AD80 (Residues: 1-893; Coverage: 100%)
Gene name: LMJF_27_1030
mL71 Chain: BQ
Molecule details ›
Chain: BQ
Length: 445 amino acids
Theoretical weight: 49.37 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QBK0 (Residues: 1-445; Coverage: 100%)
Gene name: LMJF_22_1470
mL81 Chain: BN
Molecule details ›
Chain: BN
Length: 344 amino acids
Theoretical weight: 37.74 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QAP7 (Residues: 1-344; Coverage: 100%)
Gene name: LMJF_24_0860
mL98 Chain: BE
Molecule details ›
Chain: BE
Length: 118 amino acids
Theoretical weight: 13.26 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QE16 (Residues: 1-118; Coverage: 100%)
Gene name: LMJF_18_0260
Putative ribosomal protein L14 Chain: BO
Molecule details ›
Chain: BO
Length: 190 amino acids
Theoretical weight: 21.63 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q2G1 (Residues: 1-190; Coverage: 100%)
Gene name: LMJF_34_3815
Sequence domains: Ribosomal protein L14p/L23e
mL86 Chain: At
Molecule details ›
Chain: At
Length: 183 amino acids
Theoretical weight: 21.07 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q4L5 (Residues: 1-183; Coverage: 100%)
Gene name: LMJF_33_0080
mL87 Chain: Au
Molecule details ›
Chain: Au
Length: 186 amino acids
Theoretical weight: 22.27 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q8J6 (Residues: 1-186; Coverage: 100%)
Gene name: LMJF_28_0730
mL53 Chain: Ae
Molecule details ›
Chain: Ae
Length: 311 amino acids
Theoretical weight: 35.48 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: E9ACG2 (Residues: 1-311; Coverage: 100%)
Gene name: LMJF_03_0150
mL63 Chain: Af
Molecule details ›
Chain: Af
Length: 155 amino acids
Theoretical weight: 18.1 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QJB6 (Residues: 1-155; Coverage: 100%)
Gene name: LMJF_05_0860
mL68 Chain: Ah
Molecule details ›
Chain: Ah
Length: 570 amino acids
Theoretical weight: 65.79 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QC45 (Residues: 1-570; Coverage: 100%)
Gene name: LMJF_21_1500
mL80 Chain: Ap
Molecule details ›
Chain: Ap
Length: 240 amino acids
Theoretical weight: 27.95 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q7V3 (Residues: 1-240; Coverage: 100%)
Gene name: LMJF_30_0060
mL74 Chain: Al
Molecule details ›
Chain: Al
Length: 346 amino acids
Theoretical weight: 39.39 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q1C8 (Residues: 1-346; Coverage: 100%)
Gene name: LMJF_36_2910
L51_S25_CI-B8 domain-containing protein Chain: Ab
Molecule details ›
Chain: Ab
Length: 262 amino acids
Theoretical weight: 31.23 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q6A7 (Residues: 1-262; Coverage: 100%)
Gene name: LMJF_31_1550
Sequence domains: Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain
mL42 Chain: Aa
Molecule details ›
Chain: Aa
Length: 195 amino acids
Theoretical weight: 22.17 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q183 (Residues: 1-195; Coverage: 100%)
Gene name: LMJF_36_3350
mL52,mL52 Chain: BP
Molecule details ›
Chain: BP
Length: 254 amino acids
Theoretical weight: 29.2 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QGE0 (Residues: 1-186; Coverage: 100%)
Gene name: LMJF_13_0140
mL93 Chain: Az
Molecule details ›
Chain: Az
Length: 152 amino acids
Theoretical weight: 18.86 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q4D9 (Residues: 1-152; Coverage: 100%)
Gene name: LMJF_33_0660
mL75 Chain: Am
Molecule details ›
Chain: Am
Length: 340 amino acids
Theoretical weight: 39.65 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QC22 (Residues: 1-340; Coverage: 100%)
Gene name: LMJF_21_1620
Sequence domains: PPR repeat family
mL85 Chain: As
Molecule details ›
Chain: As
Length: 249 amino acids
Theoretical weight: 27.53 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: E9ABZ5 (Residues: 1-249; Coverage: 100%)
Gene name: LMJF_01_0060
mL100 Chain: BG
Molecule details ›
Chain: BG
Length: 1347 amino acids
Theoretical weight: 146.81 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: A0A504WW14 (Residues: 1-1347; Coverage: 100%)
Gene name: CGC21_25710
mL49 Chain: Ad
Molecule details ›
Chain: Ad
Length: 237 amino acids
Theoretical weight: 26.21 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QIB3 (Residues: 1-237; Coverage: 100%)
Gene name: LMJF_08_0560
mL89 Chain: Aw
Molecule details ›
Chain: Aw
Length: 187 amino acids
Theoretical weight: 21.56 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: E9AD00 (Residues: 1-187; Coverage: 100%)
Gene name: LMJF_27_0230
Peptidyl-prolyl cis-trans isomerase Chain: BH
Molecule details ›
Chain: BH
Length: 229 amino acids
Theoretical weight: 25.31 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QBK2 (Residues: 1-229; Coverage: 100%)
Gene names: CYP6, LMJF_22_1450
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
mL72 Chain: Aj
Molecule details ›
Chain: Aj
Length: 503 amino acids
Theoretical weight: 56.55 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q728 (Residues: 1-503; Coverage: 100%)
Gene name: LMJF_30_2660
mL84 Chain: Ar
Molecule details ›
Chain: Ar
Length: 205 amino acids
Theoretical weight: 24.62 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q712 (Residues: 1-205; Coverage: 100%)
Gene name: LMJF_30_2810
mL76 Chain: An
Molecule details ›
Chain: An
Length: 331 amino acids
Theoretical weight: 38.66 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q0F5 (Residues: 1-331; Coverage: 100%)
Gene name: LMJF_36_6050
mL99 Chain: BF
Molecule details ›
Chain: BF
Length: 109 amino acids
Theoretical weight: 13.05 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QIQ1 (Residues: 1-109; Coverage: 100%)
Gene name: LMJF_07_0420
mL88 Chain: Av
Molecule details ›
Chain: Av
Length: 192 amino acids
Theoretical weight: 22.34 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QIT7 (Residues: 1-192; Coverage: 100%)
Gene name: LMJF_07_0070
mL70 Chain: BM
Molecule details ›
Chain: BM
Length: 457 amino acids
Theoretical weight: 50.75 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q703 (Residues: 1-457; Coverage: 100%)
Gene name: LMJF_30_2890
mL59/64 Chain: Ag
Molecule details ›
Chain: Ag
Length: 244 amino acids
Theoretical weight: 28.6 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q829 (Residues: 1-244; Coverage: 100%)
Gene name: LMJF_28_2360
Peptidyl-prolyl cis-trans isomerase Chain: Bl
Molecule details ›
Chain: Bl
Length: 266 amino acids
Theoretical weight: 28.92 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q1A6 (Residues: 1-266; Coverage: 100%)
Gene names: CYP10, LMJF_36_3130
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
LIM zinc-binding domain-containing protein Chain: Ax
Molecule details ›
Chain: Ax
Length: 216 amino acids
Theoretical weight: 25.17 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q7T1 (Residues: 1-216; Coverage: 100%)
Gene name: LMJF_30_0280
J domain-containing protein Chain: BS
Molecule details ›
Chain: BS
Length: 416 amino acids
Theoretical weight: 45.4 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q7K0 (Residues: 1-416; Coverage: 100%)
Gene name: LMJF_30_1030
Sequence domains: DnaJ domain
SpoU_methylase domain-containing protein Chains: BX, BY
Molecule details ›
Chains: BX, BY
Length: 569 amino acids
Theoretical weight: 63.87 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: E9AFB3 (Residues: 1-569; Coverage: 100%)
Gene name: LMJF_35_2890
Sequence domains: SpoU rRNA Methylase family
PseudoU_synth_2 domain-containing protein Chain: BZ
Molecule details ›
Chain: BZ
Length: 413 amino acids
Theoretical weight: 47.45 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: E9AC17 (Residues: 1-413; Coverage: 100%)
Gene name: LMJF_01_0280
Sequence domains: RNA pseudouridylate synthase
Acyl carrier protein Chain: CC
Molecule details ›
Chain: CC
Length: 150 amino acids
Theoretical weight: 16.63 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: E9AD06 (Residues: 1-150; Coverage: 100%)
Gene names: ACP, LMJF_27_0290
Sequence domains: Phosphopantetheine attachment site
L0R8F8 Chain: CD
Molecule details ›
Chain: CD
Length: 126 amino acids
Theoretical weight: 14.78 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QFS7 (Residues: 1-126; Coverage: 100%)
Gene name: LMJF_14_0580
G domain-containing protein Chain: BT
Molecule details ›
Chain: BT
Length: 464 amino acids
Theoretical weight: 51.66 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: E9AFZ4 (Residues: 1-464; Coverage: 100%)
Gene name: LMJF_35_5210
Sequence domains: 50S ribosome-binding GTPase
GTPase Der Chain: BU
Molecule details ›
Chain: BU
Length: 497 amino acids
Theoretical weight: 56.55 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q9G4 (Residues: 1-497; Coverage: 100%)
Gene name: LMJF_26_0270
Sequence domains:
DEAD/DEAH box helicase-like protein Chain: BW
Molecule details ›
Chain: BW
Length: 776 amino acids
Theoretical weight: 87.39 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4QHU1 (Residues: 1-776; Coverage: 100%)
Gene name: LMJF_09_0830
Sequence domains:
G domain-containing protein Chain: BV
Molecule details ›
Chain: BV
Length: 787 amino acids
Theoretical weight: 86.93 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: Q4Q4X0 (Residues: 1-787; Coverage: 100%)
Gene name: LMJF_32_3020
Sequence domains: 50S ribosome-binding GTPase
mL78 Chain: U7
Molecule details ›
Chain: U7
Length: 40 amino acids
Theoretical weight: 3.42 KDa
Source organism: Leishmania tarentolae
U6 Chain: U6
Molecule details ›
Chain: U6
Length: 187 amino acids
Theoretical weight: 13.31 KDa
Source organism: Leishmania tarentolae
U1 Chain: U1
Molecule details ›
Chain: U1
Length: 46 amino acids
Theoretical weight: 3.29 KDa
Source organism: Leishmania tarentolae
U3 Chain: U3
Molecule details ›
Chain: U3
Length: 75 amino acids
Theoretical weight: 5.35 KDa
Source organism: Leishmania tarentolae
U4 Chain: U4
Molecule details ›
Chain: U4
Length: 136 amino acids
Theoretical weight: 9.68 KDa
Source organism: Leishmania tarentolae
U5 Chain: U5
Molecule details ›
Chain: U5
Length: 94 amino acids
Theoretical weight: 6.7 KDa
Source organism: Leishmania tarentolae
mL78 Chain: BR
Molecule details ›
Chain: BR
Length: 301 amino acids
Theoretical weight: 33.02 KDa
Source organism: Leishmania tarentolae
UniProt:
  • Canonical: A0A504XZ90 (Residues: 1-301; Coverage: 100%)
Gene name: CGC21_38360
U2 Chain: U2
Molecule details ›
Chain: U2
Length: 37 amino acids
Theoretical weight: 2.65 KDa
Source organism: Leishmania tarentolae
U8 Chain: U8
Molecule details ›
Chain: U8
Length: 59 amino acids
Theoretical weight: 4.21 KDa
Source organism: Leishmania tarentolae
Ribosomal RNA Chain: 1
Molecule details ›
Chain: 1
Length: 19000 nucleotides
Theoretical weight: 6061.92 KDa
Sequence domains:
  • Trypanosomatid mitochondrial small subunit ribosomal RNA
  • Trypanosomatid mitochondrial large subunit ribosomal RNA
  • R1 Chain: R1
    Molecule details ›
    Chain: R1
    Length: 3 nucleotides
    Theoretical weight: 897 Da
    R2 Chain: R2
    Molecule details ›
    Chain: R2
    Length: 35 nucleotides
    Theoretical weight: 10.67 KDa
    R5 Chain: R5
    Molecule details ›
    Chain: R5
    Length: 5 nucleotides
    Theoretical weight: 1.49 KDa

    Ligands and Environments

    2 bound ligands:
    No modified residues

    Experiments and Validation Details

    Entry percentile scores
    Resolution: 3.4Å
    Relevant EMDB volumes: EMD-11821