EMDB EMD-0704

Single particle reconstruction
3.25Å resolution

Structure of LbCas12a-crRNA complex bound to AcrVA4 (form A complex)

Map released:
Overview of EMD-0704
Source organisms:
Fitted atomic model: 6kl9
Related EM entry by publication: EMD-0705
Primary publication:
Structural insight into multistage inhibition of CRISPR-Cas12a by AcrVA4.
Peng R, Li Z, Xu Y, He S, Peng Q, Wu LA, Wu Y, Qi J, Wang P, Shi Y, Gao GF
Proc.Natl.Acad.Sci.USA - (2019)
PMID: 31467167

Function and Biology Details

Sample name: LbCas12a-crRNA complex bound to AcrVA4 (form A complex)
Ligands: MAGNESIUM ION, water
Nucleic acid: RNA (42-MER)
Proteins: LbCas12a-crRNA complex bound to AcrVA4 (form A complex), LbCas12a, AcrVA4

Experimental Information Details

Resolution: 3.25Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)