6kl9

Electron Microscopy
3.25Å resolution

Structure of LbCas12a-crRNA complex bound to AcrVA4 (form A complex)

Released:
Primary publication:
Structural insight into multistage inhibition of CRISPR-Cas12a by AcrVA4.
Proc. Natl. Acad. Sci. U.S.A. (2019)
PMID: 31467167
Related structures: EMD-0704

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
LbCas12a Chain: A
Molecule details ›
Chain: A
Length: 1228 amino acids
Theoretical weight: 143.89 KDa
Source organism: Lachnospiraceae bacterium
Expression system: Escherichia coli BL21
AcrVA4 Chains: B, C
Molecule details ›
Chains: B, C
Length: 234 amino acids
Theoretical weight: 27.37 KDa
Source organism: Moraxella bovoculi
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0U2APF4 (Residues: 1-234; Coverage: 100%)
Gene name: AAX07_09545
RNA (42-MER) Chain: G
Molecule details ›
Chain: G
Length: 42 nucleotides
Theoretical weight: 13.48 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.25Å
Relevant EMDB volumes: EMD-0704
Expression system: Escherichia coli BL21