EMDB EMD-0585

Single particle reconstruction
3.3Å resolution

Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium

Map released:
Overview of EMD-0585
Source organisms:
Fitted atomic model: 6o0z
Related EM entry by publication: EMD-0583, EMD-0584
Primary publication:
Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9
Zhu X, Clarke R, Puppala AK, Chittori S, Merk A, Merrill BJ, Simonovic M, Subramaniam S
Nat.Struct.Mol.Biol. - (2019)

Function and Biology Details

Sample name: Cas9-sgRNA-DNA ternary complex
Nucleic acids: single guide RNA, target strand DNA, non-target strand DNA
Proteins: Cas9-sgRNA-DNA ternary complex, CRISPR-associated endonuclease Cas9/Csn1

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)