6o0z

Electron Microscopy
3.3Å resolution

Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1372 amino acids
Theoretical weight: 158.99 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
target strand DNA Chain: C
Molecule details ›
Chain: C
Length: 40 nucleotides
Theoretical weight: 12.18 KDa
Source organism: synthetic construct
Expression system: Not provided
non-target strand DNA Chain: D
Molecule details ›
Chain: D
Length: 40 nucleotides
Theoretical weight: 12.45 KDa
Source organism: synthetic construct
Expression system: Not provided
single guide RNA Chain: B
Molecule details ›
Chain: B
Length: 102 nucleotides
Theoretical weight: 33.06 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-0585
Expression systems:
  • Escherichia coli
  • Not provided