EMDB EMD-0258

Single particle reconstruction
4.0Å resolution

Cryo-EM structure of the KdpFABC complex in an E2 inward-facing state (state 2)

Map released:
Overview of EMD-0258
Source organism: Escherichia coli (strain K12) [83333]
Fitted atomic model: 6hrb
Related EM entry by publication: EMD-0257
Primary publication:
Cryo-EM structures of KdpFABC suggest a K+transport mechanism via two inter-subunit half-channels.
Stock C, Hielkema L, Tascon I, Wunnicke D, Oostergetel GT, Azkargorta M, Paulino C, Hanelt I
Nat Commun 9 4971-4971 (2018)
PMID: 30478378

Function and Biology Details

Sample name: KdpFABC
Ligand: POTASSIUM ION
Proteins: KdpFABC, Potassium-transporting ATPase potassium-binding subunit, Potassium-transporting ATPase ATP-binding subunit, Potassium-transporting ATPase KdpC subunit, Potassium-transporting ATPase KdpF subunit

Experimental Information Details

Resolution: 4.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TALOS ARCTICA
Detector: GATAN K2 SUMMIT (4k x 4k)