6hrb

Electron Microscopy
4Å resolution

Cryo-EM structure of the KdpFABC complex in an E2 inward-facing state (state 2)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Potassium-transporting ATPase potassium-binding subunit Chain: A
Molecule details ›
Chain: A
Length: 557 amino acids
Theoretical weight: 59.22 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03959 (Residues: 1-557; Coverage: 100%)
Gene names: JW0686, b0698, kdpA
Sequence domains: Potassium-transporting ATPase A subunit
Potassium-transporting ATPase ATP-binding subunit Chain: B
Molecule details ›
Chain: B
Length: 682 amino acids
Theoretical weight: 72.35 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03960 (Residues: 1-682; Coverage: 100%)
Gene names: JW0685, b0697, kdpB
Sequence domains:
Potassium-transporting ATPase KdpC subunit Chain: C
Molecule details ›
Chain: C
Length: 190 amino acids
Theoretical weight: 20.28 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03961 (Residues: 1-190; Coverage: 100%)
Gene names: JW0684, b0696, kdpC
Sequence domains: K+-transporting ATPase, c chain
Potassium-transporting ATPase KdpF subunit Chain: D
Molecule details ›
Chain: D
Length: 29 amino acids
Theoretical weight: 3.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P36937 (Residues: 1-29; Coverage: 100%)
Gene names: JW0687, b4513, kdpF
Sequence domains: F subunit of K+-transporting ATPase (Potass_KdpF)

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-0258
Expression system: Escherichia coli