Substrates for peptidase S08.009: subtilisin Ak1

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB MERNUM
Ala-Ala-NHPhNO2 Ala-Ala+NHPhNO2 S - - Ala Ala NAN - - - Toogood et al., 2004
Ala-Ala-Phe-NHPhNO2 Ala-Ala-Phe+NHPhNO2 S - Ala Ala Phe NAN - - - Toogood et al., 2004
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln+His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Phe Val Asn Gln His Leu Cya Gly Peek et al., 1993 24113
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu+Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N His Leu Val Glu Ala Leu Tyr Leu Peek et al., 1993 24114
insulin B-chain (oxidized) P01317 25-54 Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu+Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala N Val Glu Ala Leu Tyr Leu Val Cya Peek et al., 1993 24115
Suc-Ala-Ala-Ala-NHPhNO2 Suc-Ala-Ala-Ala+NHPhNO2 S Suc Ala Ala Ala NAN - - - Toogood et al., 2004
Suc-Ala-Ala-Pro-Phe-NHPhNO2 Suc-Ala-Ala-Pro-Phe+NHPhNO2 S Ala Ala Pro Phe NAN - - - Toogood et al., 2004
Suc-Tyr-Leu-Val-NHPhNO2 Suc-Tyr-Leu-Val+NHPhNO2 S Suc Tyr Leu Val NAN - - - Toogood et al., 2004
Z-Ala-OPhNO2 Z-Ala+OPhNO2 S - - Z Ala ONA - - - Toogood et al., 2004
Z-Gly-OPhNO2 Z-Gly+OPhNO2 S - - Z Gly ONA - - - Toogood et al., 2004
Z-Leu-OPhNO2 Z-Leu+OPhNO2 S - - Z Leu ONA - - - Toogood et al., 2004
Z-Nle-OPhNO2 Z-Nle+OPhNO2 S - - Z Nle ONA - - - Toogood et al., 2004