Substrates for peptidase C13.001: legumain (plant beta form)

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB MERNUM
Boc-Asn-OPhNO2 Boc-Asn+OPhNO2 S - - Boc Asn ONA - - - Hara-Nishimura, 2004
Dnp-Pro-Glu-Ala-Asn-NH2 Dnp-Pro-Glu-Ala-Asn+NH2 S Pro Glu Ala Asn NH2 - - - Hara-Nishimura, 2004
Equistatin P81439 1-199 Peptide-Asn13+Ser-Peptide P NT Ala Ser Ala Asn Ser Gly Leu Ile Outchkourov et al., 2003
Equistatin P81439 1-199 Peptide-Asn82+Val-Peptide P NT Ile Ile Val Asn Val Pro Gly Trp Outchkourov et al., 2003
Glu-Thr-Arg-Asn-Gly-Val-Glu-Glu Glu-Thr-Arg-Asn+Gly-Val-Glu-Glu N Glu Thr Arg Asn Gly Val Glu Glu Hara-Nishimura, 2004
multicystatin Q9MB08 1-282 peptide-Asn15+Ser-peptide P Asp Phe Ala Asn Ser Ile Val Ile Kouzuma et al., 2000
multicystatin Q9MB08 1-282 peptide-Asn205+Ser-peptide P Asp Phe Ala Asn Ser Leu Val Ile Kouzuma et al., 2000
phaseolin, alpha-type P07219 25-436 peptide-Asn250+Thr-peptide P NT Lys Gln Asp Asn Thr Ile Gly Asn Zakharov et al., 2004
Phaseolin, beta-type P02853 1-421 Peptide-Asn244+Thr-Peptide P NT Lys Gln Asp Asn Thr Ile Gly Asn Senyuk et al., 1998
Phaseolin, beta-type P02853 1-421 Peptide-Asn259+Ser-Peptide P NT Arg Thr Asp Asn Ser Leu Asn Val Senyuk et al., 1998
Phaseolin, beta-type P02853 1-421 Peptide-Asn127+Pro-Peptide P NT Thr Ser Asp Asn Pro Ile Phe Ser Senyuk et al., 1998
Ser-Glu-Ser-Glu-Asn-Gly-Leu-Glu-Glu-Thr Ser-Glu-Ser-Glu-Asn+Gly-Leu-Glu-Glu-Thr N Glu Ser Glu Asn Gly Leu Glu Glu Hara-Nishimura, 2004
Vacuolar-processing enzyme gamma-isozyme Q39119 5-494 Peptide-Asn50+Asp-Peptide P NT Pro Ala Glu Asn Asp Asp Asp Ser Kuroyanagi et al., 2002
Vignain P12412 1-131 Peptide-Asn105+His-Peptide P NT Ser Lys Val Asn His His Lys Met Okamoto et al., 1999
Z-Ala-Ala-Asn-NHMec Z-Ala-Ala-Asn+NHMec S Z Ala Ala Asn AMC - - - Hara-Nishimura, 2004