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Literature for peptidase T01.006: HslV component of HslUV peptidase

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors

(Topics flags: S Structure, K Knockout, P Specificity, I Inhibitor, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Lu,Q., Tang,Z., Guan,G., Yang,D., Liu,C., Du,C., Zhi,K., Wang,H. and Lu,F.
    Deletion of the hslV gene alleviates the virulence and lethality of Salmonella Typhimurium in hosts by modulating lon and T3SS-1
    Virulence (2025) 16, 2580174-2580174. PubMed  Europe PubMed DOI  PMC  EPMC
    2. 2023
  2. Hamid,M., Salar,U., Rashid,Y., Azim,M.K., Khan,K.M., Naz,S., Aziz,T., Alharbi,M., Alshammari,A. and Alasmari,A.F.
    Determining the 3-substituted Coumarins inhibitory potential against the HslV protease of E. coli
    Eur Rev Med Pharmacol Sci (2023) 27, 9169-9182. PubMed  Europe PubMed DOI  I
    3. 2019
  3. Chang,C.Y., Weng,Y.T., Hwang,L.Y., Hu,H.T., Shih,P.S., Kuan,J.E., Wu,K.F. and Wu,W.F.
    Specific regions of the SulA protein recognized and degraded by the ATP-dependent ClpYQ (HslUV) protease in Escherichia coli
    Microbiol Res (2019) 220, 21-31. PubMed  Europe PubMed DOI
  4. Fuchs,A.C.D. and Hartmann,M.D.
    On the origins of symmetry and modularity in the proteasome family: symmetry transitions are pivotal in the evolution and functional diversification of self-compartmentalizing proteases
    Bioessays (2019) , e1800237-e1800237. PubMed  Europe PubMed DOI
    5. 2018
  5. Yu,Y., Yan,F., He,Y., Qin,Y., Chen,Y., Chai,Y. and Guo,J.H.
    The ClpY-ClpQ protease regulates multicellular development in Bacillus subtilis
    Microbiology (Reading) (2018) 164, 848-862. PubMed  Europe PubMed DOI
    6. 2017
  6. Baytshtok,V., Chen,J., Glynn,S.E., Nager,A.R., Grant,R.A., Baker,T.A. and Sauer,R.T.
    Covalently linked HslU hexamers support a probabilistic mechanism that links ATP hydrolysis to protein unfolding and translocation
    J Biol Chem (2017) 292, 5695-5704. PubMed  Europe PubMed DOI
  7. Dong,S.L., Hu,W.L., Ge,Y.M., Ojcius,D.M., Lin,X. and Yan,J.
    A leptospiral AAA+ chaperone-Ntn peptidase complex, HslUV, contributes to the intracellular survival of Leptospira interrogans in hosts and the transmission of leptospirosis
    Emerg Microbes Infect (2017) 6, e105-e105. PubMed  Europe PubMed DOI  PMC  EPMC
  8. Tsai,C.H., Ho,Y.H., Sung,T.C., Wu,W.F. and Chen,C.S.
    Escherichia coli proteome microarrays identified the substrates of ClpYQ protease
    Mol Cell Proteomics (2017) 16, 113-120. PubMed  Europe PubMed DOI  P
    9. 2016
  9. Chang,C.Y., Hu,H.T., Tsai,C.H. and Wu,W.F.
    The degradation of RcsA by ClpYQ(HslUV) protease in Escherichia coli
    Microbiol Res (2016) 184, 42-50. PubMed  Europe PubMed DOI
    10. 2014
  10. Hwang,L.-Y., Hu,H.-T., Tsai,C.-H. and Wu,W.-F.
    The Escherichia coli ClpYQ protease recognizes the C-terminal tail of SulA
    Taiwanese J Agric Chem Food Sci (2014) 52, 48-58.  P
  11. Shi,L. and Kay,L.E.
    Tracing an allosteric pathway regulating the activity of the HslV protease
    Proc Natl Acad Sci U S A (2014) 111, 2140-2145. PubMed  Europe PubMed DOI
    12. 2013
  12. Jain,S., Rathore,S., Asad,M., Hossain,M.E., Sinha,D., Datta,G. and Mohmmed,A.
    The prokaryotic ClpQ protease plays a key role in growth and development of mitochondria in Plasmodium falciparum
    Cell Microbiol (2013) 15, 1660-1673. PubMed  Europe PubMed DOI
  13. Park,E., Lee,J.W., Yoo,H.M., Ha,B.H., An,J.Y., Jeon,Y.J., Seol,J.H., Eom,S.H. and Chung,C.H.
    Structural alteration in the pore motif of the bacterial 20S proteasome homolog HslV leads to uncontrolled protein degradation
    J Mol Biol (2013) 425, 2940-2954. PubMed  Europe PubMed DOI
    14. 2012
  14. Chrobak,M., Forster,S., Meisel,S., Pfefferkorn,R., Forster,F. and Clos,J.
    Leishmania donovani HslV does not interact stably with HslU proteins
    Int J Parasitol (2012) 42, 329-339. PubMed  Europe PubMed DOI
  15. Park,E., Yoo,S.J., Seong,I.S., Goldberg,A.L. and Chung,C.H.
    HslVU protease
    [ISSN:978-0-12-407742-3] (2012) 3, 3677-3683. DOI
  16. Rashid,Y., Kamran Azim,M., Saify,Z.S., Khan,K.M. and Khan,R.
    Small molecule activators of proteasome-related HslV peptidase
    Bioorg Med Chem Lett (2012) 22, 6089-6094. PubMed  Europe PubMed DOI  I
  17. Sundar,S., Baker,T.A. and Sauer,R.T.
    The I domain of the AAA+ HslUV protease coordinates substrate binding, ATP hydrolysis, and protein degradation
    Protein Sci (2012) 21, 188-198. PubMed  Europe PubMed DOI
    18. 2011
  18. Rathore,S., Jain,S., Sinha,D., Gupta,M., Asad,M., Srivastava,A., Narayanan,M.S., Ramasamy,G., Chauhan,V.S., Gupta,D. and Mohmmed,A.
    Disruption of a mitochondrial protease machinery in Plasmodium falciparum is an intrinsic signal for parasite cell death
    Cell Death Dis (2011) 2, e231-e231. PubMed  Europe PubMed DOI
  19. Sauer,R.T. and Baker,T.A.
    AAA+ Proteases: ATP-fueled machines of protein destruction
    Annu Rev Biochem (2011) 80, 587-612. PubMed  Europe PubMed DOI
    20. 2010
  20. Sundar,S., McGinness,K.E., Baker,T.A. and Sauer,R.T.
    Multiple sequence signals direct recognition and degradation of protein substrates by the AAA+ protease HslUV
    J Mol Biol (2010) 403, 420-429. PubMed  Europe PubMed DOI  P
    21. 2009
  21. Koodathingal,P., Jaffe,N.E., Kraut,D.A., Prakash,S., Fishbain,S., Herman,C. and Matouschek,A.
    ATP-dependent proteases differ substantially in their ability to unfold globular proteins
    J Biol Chem (2009) 284, 18674-18684. PubMed  Europe PubMed DOI  PMC  EPMC
  22. Lee,J.W., Park,E., Jeong,M.S., Jeon,Y.J., Eom,S.H., Seol,J.H. and Chung,C.H.
    HslVU ATP-dependent protease utilizes maximally six among twelve threonine active sites during proteolysis
    J Biol Chem (2009) 284, 33475-33484. PubMed  Europe PubMed DOI
    23. 2008
  23. Inobe,T. and Matouschek,A.
    Protein targeting to ATP-dependent proteases
    Curr Opin Struct Biol (2008) 18, 43-51. PubMed  Europe PubMed DOI  PMC  EPMC  V
  24. Park,E., Lee,J.W., Eom,S.H., Seol,J.H. and Chung,C.H.
    Binding of MG132 or deletion of the Thr active sites in HslV subunits increases the affinity of HslV protease for HslU ATPase and makes this interaction nucleotide-independent
    J Biol Chem (2008) 283, 33258-33266. PubMed  Europe PubMed DOI  PMC  EPMC  I
  25. Yakamavich,J.A., Baker,T.A. and Sauer,R.T.
    Asymmetric nucleotide transactions of the HslUV protease
    J Mol Biol (2008) 380, 946-957. PubMed  Europe PubMed DOI  PMC  EPMC
    26. 2007
  26. Azim,M.K. and Noor,S.
    Characterization of protomer interfaces in HslV protease; the bacterial homologue of 20S proteasome
    Protein J (2007) 26, 213-219. PubMed  Europe PubMed DOI
  27. Lee,J.W., Park,E., Bang,O., Eom,S.H., Cheong,G.W., Chung,C.H. and Seol,J.H.
    Nucleotide triphosphates inhibit the degradation of unfolded proteins by HslV peptidase
    Mol Cells (2007) 23, 252-257. PubMed  Europe PubMed
    28. 2006
  28. [YEAR:4-10-2006]Ruiz-Gonzalez,M.X. and Marin,I.
    Proteasome-related HslU and HslV genes typical of eubacteria are widespread in eukaryotes
    J Mol Evol (4-10-2006) 63, 504-512. PubMed  Europe PubMed DOI
    29. 2005
  29. [YEAR:31-3-2005]Azim,M.K., Goehring,W., Song,H.K., Ramachandran,R., Bochtler,M. and Goettig,P.
    Characterization of the HslU chaperone affinity for HslV protease
    Protein Sci (31-3-2005) 14, 1357-1362. PubMed  Europe PubMed DOI  PMC  EPMC
  30. Azim,M.K., Goehring,W., Song,H.K., Ramachandran,R., Bochtler,M. and Goettig,P.
    Characterization of the HsIU chaperone affinity for HsIV protease (vol 14, pg 1357, 2005)
    Protein Sci (2005) 14, 2484-2484. PubMed  Europe PubMed DOI  PMC  EPMC
  31. [YEAR:6-2-2005]Burton,R.E., Baker,T.A. and Sauer,R.T.
    Nucleotide-dependent substrate recognition by the AAA+ HslUV protease
    Nat Struct Mol Biol (6-2-2005) 12, 245-251. PubMed  Europe PubMed DOI
  32. [YEAR:21-4-2005]Frees,D., Thomsen,L.E. and Ingmer,H.
    Staphylococcus aureus ClpYQ plays a minor role in stress survival
    Arch Microbiol (21-4-2005) 183, 286-291. PubMed  Europe PubMed DOI  K
  33. [YEAR:22-4-2005]Park,E., Rho,Y.M., Koh,O.J., Ahn,S.W., Seong,I.S., Song,J.J., Bang,O., Seol,J.H., Wang,J., Eom,S.H. and Chung,C.H.
    Role of the GYVG pore motif of HslU ATPase in protein unfolding and translocation for degradation by HslV peptidase
    J Biol Chem (22-4-2005) 280, 22892-22898. PubMed  Europe PubMed DOI
  34. Wang,J., Rho,S.H., Park,H.H. and Eom,S.H.
    Correction of X-ray intensities from an HslV-HslU co-crystal containing lattice-translocation defects
    Acta Crystallogr D Biol Crystallogr (2005) 61, 932-941. PubMed  Europe PubMed DOI  S
    35. 2004
  35. Huang,H.C., Goldberg,A.L., Yoo,S.J., Seong,I.S. and Chung,C.H.
    HslVU protease
    [ISSN:0-12-079610-4] (2004) 2, 2064-2068.  V
  36. Kwon,A.R., Trame,C.B. and McKay,D.B.
    Kinetics of protein substrate degradation by HslUV
    J Struct Biol (2004) 146, 141-147. PubMed  Europe PubMed DOI
    37. 2003
  37. [YEAR:4-7-2003]Kwon,A.R., Kessler,B.M., Overkleeft,H.S. and McKay,D.B.
    Structure and reactivity of an asymmetric complex between HslV and I-domain deleted HslU, a prokaryotic homolog of the eukaryotic proteasome
    J Mol Biol (4-7-2003) 330, 185-195. PubMed  Europe PubMed DOI
  38. Lee,Y.Y., Chang,C.F., Kuo,C.L., Chen,M.C., Yu,C.H., Lin,P.I. and Wu,W.F.
    Subunit oligomerization and substrate recognition of the Escherichia coli ClpYQ (HslUV) protease implicated by in vivo protein-protein interactions in the yeast two-hybrid system
    J Bacteriol (2003) 185, 2393-2401. PubMed  Europe PubMed DOI  PMC  EPMC
  39. [YEAR:23-10-2003]Nishii,W. and Takahashi,K.
    Determination of the cleavage sites in SulA, a cell division inhibitor, by the ATP-dependent HslVU protease from Escherichia coli
    FEBS Lett (23-10-2003) 553, 351-354. PubMed  Europe PubMed DOI
    40. 2002
  40. [YEAR:28-5-2002]Ramachandran,R., Hartmann,C., Song,H.K., Huber,R. and Bochtler,M.
    Functional interactions of HslV (ClpQ) with the ATPase HslU (ClpY)
    Proc Natl Acad Sci U S A (28-5-2002) 99, 7396-7401. PubMed  Europe PubMed DOI  PMC  EPMC
  41. [YEAR:19-7-2002]Seong,I.S., Kang,M.S., Choi,M.K., Lee,J.W., Koh,O.J., Wang,J., Eom,S.H. and Chung,C.H.
    The C-terminal tails of HslU ATPase act as a molecular switch for activation of HslV peptidase
    J Biol Chem (19-7-2002) 277, 25976-25982. PubMed  Europe PubMed DOI
  42. [YEAR:3-5-2002]Sousa,M.C., Kessler,B.M., Overkleeft,H.S. and McKay,D.B.
    Crystal structure of HslUV complexed with a vinyl sulfone inhibitor: corroboration of a proposed mechanism of allosteric activation of HslV by HslU
    J Mol Biol (3-5-2002) 318, 779-785. PubMed  Europe PubMed DOI  S  I
    43. 2001
  43. Bochtler,M., Song,H.K., Hartmann,C., Ramachandran,R. and Huber,R.
    The quaternary arrangement of HslU and HslV in a cocrystal: a response to Wang, Yale
    J Struct Biol (2001) 135, 281-293. PubMed  Europe PubMed DOI  S
  44. Sousa,M.C. and McKay,D.B.
    Structure of Haemophilus influenzae HslV protein at 1.9 A resolution, revealing a cation-binding site near the catalytic site
    Acta Crystallogr D Biol Crystallogr (2001) 57, 1950-1954. PubMed  Europe PubMed DOI
  45. Wang,J.
    A corrected quaternary arrangement of the peptidase HslV and ATPase HslU in a cocrystal structure
    J Struct Biol (2001) 134, 15-24. PubMed  Europe PubMed DOI  S
  46. Wang,J., Song,J.J., Franklin,M.C., Kamtekar,S., Im,Y.J., Rho,S.H., Seong,I.S., Lee,C.S., Chung,C.H. and Eom,S.H.
    Crystal structures of the HslVU peptidase-ATPase complex reveal an ATP-dependent proteolysis mechanism
    Structure (2001) 9, 177-184. PubMed  Europe PubMed DOI  S
    47. 2000
  47. [YEAR:17-2-2000]Bochtler,M., Hartmann,C., Song,H.K., Bourenkov,G.P., Bartunik,H.D. and Huber,R.
    The structures of HsIU and the ATP-dependent protease HsIU-HsIV
    Nature (17-2-2000) 403, 800-805. PubMed  Europe PubMed DOI  S
  48. [YEAR:21-7-2000]Seong,I.S., Oh,J.Y., Lee,J.W., Tanaka,K. and Chung,C.H.
    The HslU ATPase acts as a molecular chaperone in prevention of aggregation of SulA, an inhibitor of cell division in Escherichia coli
    FEBS Lett (21-7-2000) 477, 224-229. PubMed  Europe PubMed DOI
  49. [YEAR:10-11-2000]Sousa,M.C., Trame,C.B., Tsuruta,H., Wilbanks,S.M., Reddy,V.S. and McKay,D.B.
    Crystal and solution structures of an HslUV protease-chaperone complex
    Cell (10-11-2000) 103, 633-643. PubMed  Europe PubMed  S
    50. 1999
  50. Kanemori,M., Yanagi,H. and Yura,T.
    The ATP-dependent HslVU/ClpQY protease participates in turnover of cell division inhibitor SulA in Escherichia coli
    J Bacteriol (1999) 181, 3674-3680. PubMed  Europe PubMed  PMC  EPMC
  51. Schmidt,M., Lupas,A.N. and Finley,D.
    Structure and mechanism of ATP-dependent proteases
    Curr Opin Chem Biol (1999) 3, 584-591. PubMed  Europe PubMed DOI
  52. [YEAR:30-7-1999]Seong,I.S., Oh,J.Y., Yoo,S.J., Seol,J.H. and Chung,C.H.
    ATP-dependent degradation of SulA, a cell division inhibitor, by the HslVU protease in Escherichia coli
    FEBS Lett (30-7-1999) 456, 211-214. PubMed  Europe PubMed DOI
  53. Wu,W.F., Zhou,Y. and Gottesman,S.
    Redundant in vivo proteolytic activities of Escherichia coli lon and the ClpYQ (HslUV) protease
    J Bacteriol (1999) 181, 3681-3687. PubMed  Europe PubMed  PMC  EPMC
    54. 1998
  54. [YEAR:2-1-1998]Hilliard,J.J., Maurizi,M.R. and Simon,L.D.
    Isolation and characterization of the phage T4 PinA protein, an inhibitor of the ATP-dependent Lon protease of Escherichia coli
    J Biol Chem (2-1-1998) 273, 518-523. PubMed  Europe PubMed DOI
  55. [YEAR:4-9-1998]Yoo,S.J., Kim,H.H., Shin,D.H., Lee,C.S., Seong,I.S., Seol,J.H., Shimbara,N., Tanaka,K. and Chung,C.H.
    Effects of the Cys mutations on structure and function of the ATP-dependent HslVU protease in Escherichia coli. The Cys287 to Val mutation in HslU uncouples the ATP-dependent proteolysis by HslVU from ATP hydrolysis
    J Biol Chem (4-9-1998) 273, 22929-22935. PubMed  Europe PubMed DOI
    56. 1997
  56. [YEAR:10-6-1997]Bochtler,M., Ditzel,L., Groll,M. and Huber,R.
    Crystal structure of heat shock locus V (HslV) from Escherichia coli
    Proc Natl Acad Sci U S A (10-6-1997) 94, 6070-6074. PubMed  Europe PubMed DOI  PMC  EPMC
  57. [YEAR:24-6-1997]Bogyo,M., McMaster,J.S., Gaczynska,M., Tortorella,D., Goldberg,A.L. and Ploegh,H.
    Covalent modification of the active site threonine of proteasomal beta subunits and the Escherichia coli homolog HslV by a new class of inhibitors
    Proc Natl Acad Sci U S A (24-6-1997) 94, 6629-6634. PubMed  Europe PubMed  PMC  EPMC  I
  58. Goldberg,A.L., Akopian,T.N., Kisselev,A.F., Lee,D.H. and Rohrwild,M.
    New insights into the mechanisms and importance of the proteasome in intracellular protein degradation
    Biol Chem (1997) 378, 131-140. PubMed  Europe PubMed
  59. [YEAR:22-8-1997]Huang,H. and Goldberg,A.L.
    Proteolytic activity of the ATP-dependent protease HslVU can be uncoupled from ATP hydrolysis
    J Biol Chem (22-8-1997) 272, 21364-21372. PubMed  Europe PubMed DOI
  60. Khattar,M.M.
    Overexpression of the hslVU operon suppresses SOS-mediated inhibition of cell division in Escherichia coli
    FEBS Lett (1997) 414, 402-404. PubMed  Europe PubMed DOI
  61. Rohrwild,M., Pfeifer,G., Santarius,U., Muller,S.A., Huang,H.C., Engel,A., Baumeister,W. and Goldberg,A.L.
    The ATP-dependent HslVU protease from Escherichia coli is a four-ring structure resembling the proteasome
    Nat Struct Biol (1997) 4, 133-139. PubMed  Europe PubMed DOI  S
  62. Yoo,S.J., Shim,Y.K., Seong,I.S., Seol,J.H., Kang,M.S. and Chung,C.H.
    Mutagenesis of two N-terminal Thr and five Ser residues in HslV, the proteolytic component of the ATP-dependent HslVU protease
    FEBS Lett (1997) 412, 57-60. PubMed  Europe PubMed DOI
    63. 1996
  63. Kessel,M., Wu,W., Gottesman,S., Kocsis,E., Steven,A.C. and Maurizi,M.R.
    Six-fold rotational symmetry of ClpQ, the E. coli homolog of the 20S proteasome, and its ATP-dependent activator, ClpY
    FEBS Lett (1996) 398, 274-278. PubMed  Europe PubMed DOI
  64. Missiakas,D., Schwager,F., Betton,J.M., Georgopoulos,C. and Raina,S.
    Identification and characterization of HsIV HsIU (ClpQ ClpY) proteins involved in overall proteolysis of misfolded proteins in Escherichia coli
    EMBO J (1996) 15, 6899-6909. PubMed  Europe PubMed  PMC  EPMC
  65. Rohrwild,M., Coux,O., Huang,H.C., Moerschell,R.P., Yoo,S.J., Seol,J.H., Chung,C.H. and Goldberg,A.L.
    HslV-HslU: A novel ATP-dependent protease complex in Escherichia coli related to the eukaryotic proteasome
    Proc Natl Acad Sci U S A (1996) 93, 5808-5813. PubMed  Europe PubMed  PMC  EPMC
  66. Shin,D.H., Yoo,S.J., Shim,Y.K., Seol,J.H., Kang,M.S. and Chung,C.H.
    Mutational analysis of the ATP-binding site in HslU, the ATPase component of HslVU protease in Escherichia coli
    FEBS Lett (1996) 398, 151-154. PubMed  Europe PubMed DOI
  67. Yoo,S.J., Seol,J.H., Kang,M.S. and Chung,C.H.
    Poly-L-lysine activates both peptide and ATP hydrolysis by the ATP-dependent HslVU protease in Escherichia coli
    Biochem Biophys Res Commun (1996) 229, 531-535. PubMed  Europe PubMed DOI
  68. Yoo,S.J., Seol,J.H., Shin,D.H., Rohrwild,M., Kang,M.S., Tanaka,K., Goldberg,A.L. and Chung,C.H.
    Purification and characterization of the heat shock proteins HslV and HslU that form a new ATP-dependent protease in Escherichia coli
    J Biol Chem (1996) 271, 14035-14040. PubMed  Europe PubMed DOI
    69. 1995
  69. Gottesman,S., Wickner,S., Jubete,Y., Singh,S.K., Kessel,M. and Maurizi,M.
    Selective, energy-dependent proteolysis in Escherichia coli
    Cold Spring Harb Symp Quant Biol (1995) 60, 533-548. PubMed  Europe PubMed
    70. 1993
  70. [YEAR:30-11-1993]Chuang,S.E., Burland,V., Plunkett,G., III, Daniels,D.L. and Blattner,F.R.
    Sequence analysis of four new heat-shock genes constituting the hslTS/ibpAB and hslVU operons in Escherichia coli
    Gene (30-11-1993) 134, 1-6. PubMed  Europe PubMed DOI
  71. [YEAR:25-10-1993]Gottesman,S., Clark,W.P., de Crecy-Lagard,V. and Maurizi,M.R.
    ClpX, an alternative subunit for the ATP-dependent Clp protease of Escherichia coli. Sequence and in vivo activities
    J Biol Chem (25-10-1993) 268, 22618-22626. PubMed  Europe PubMed

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